Fluorescent Lateral Flow Immunoassay Based on Quantum Dots NanobeadsLingzhi Fan *1, Yue Luo *2, Wannian Yan 1, Huanxing Han 3, Pengfei Zhang 1
1Department of Central Laboratory, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, 2Department of Dermatology, Shanghai Skin Disease Hospital, Institute of Dermatology, School of Medicine, Tongji University, 3Department of Pharmacy, Changzheng Hospital, Naval Medical University
Here, we describe a protocol for the preparation of quantum dot nanobeads (QDNB) and the detection of disease biomarkers using QDNB-based lateral flow immunoassay strips. The test results can be qualitatively assessed under UV light illumination and quantitatively measured using a fluorescent strip reader within 15 min.
