Naval Medical University

2 ARTICLES PUBLISHED IN JoVE

Medicine

A Modified Method for Intrathecal Catheterization in Rats

Lijun Yin^1,2, Quan Zhou³, Wenwu Liu⁴, Lize Xiong¹, Hui Chen¹

¹Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation, Clinical Research Center for Anesthesiology and Perioperative Medicine, Translational Research Institute of Brain and Brain-Like Intelligence, Department of Anesthesiology and Perioperative Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, ²Department of Anesthesiology, Women and Children's Hospital of Ningbo University, ³Department of Naval Diving Medicine, Naval Medical Center, Naval Medical University, ⁴Department of Diving and Hyperbaric Medicine, Naval Medical Center, Naval Medical University

Herein, we introduce a modified method for intrathecal catheterization in rats that represents a simple, convenient, and reliable approach for repetitive intrathecal drug administration.

Biochemistry

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Lingzhi Fan^*1, Yue Luo^*2, Wannian Yan¹, Huanxing Han³, Pengfei Zhang¹

¹Department of Central Laboratory, Shanghai Skin Disease Hospital, School of Medicine, Tongji University, ²Department of Dermatology, Shanghai Skin Disease Hospital, Institute of Dermatology, School of Medicine, Tongji University, ³Department of Pharmacy, Changzheng Hospital, Naval Medical University

Here, we describe a protocol for the preparation of quantum dot nanobeads (QDNB) and the detection of disease biomarkers using QDNB-based lateral flow immunoassay strips. The test results can be qualitatively assessed under UV light illumination and quantitatively measured using a fluorescent strip reader within 15 min.