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Washington University in Saint Louis

2 ARTICLES PUBLISHED IN JoVE

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Biology

Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis
Jeffrey R. Millman 1,2, Teresa Doggett 3, Christina Thebeau 3, Sheng Zhang 3, Clay F. Semenkovich 1, Rithwick Rajagopal 3
1Division of Metabolism, Endocrinology and Lipid Research, Department of Medicine, Washington University School of Medicine, 2Department of Biomedical Engineering, Washington University in Saint Louis, 3Department of Ophthalmology and Visual Science, Washington University School of Medicine

This technique describes real time recording of oxygen consumption and extracellular acidification rates in explanted mouse retinal tissues using an extracellular flux analyzer.

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Engineering

Fabrication of High-Quality Whispering Gallery Mode Microbubble Resonators
Maxwell R. Adolphson 1, Jie Liao 1, Abraham Qavi 1,2, Gaya Amarasinghe 3, Lan Yang 1
1Department of Electrical and Systems Engineering, Washington University in Saint Louis, 2Department of Pathology and Laboratory Medicine, University of California, Irvine, 3Department of Pathology and Immunology, Washington University School of Medicine

Here, we demonstrate a robust and standardized protocol for fabricating high-quality factor (Q-factor) Whispering Gallery Mode (WGM) Microbubble resonators (MBRs) with a precision glass processing machine (PGP).

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