Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux AnalysisJeffrey R. Millman 1,2, Teresa Doggett 3, Christina Thebeau 3, Sheng Zhang 3, Clay F. Semenkovich 1, Rithwick Rajagopal 3
1Division of Metabolism, Endocrinology and Lipid Research, Department of Medicine, Washington University School of Medicine, 2Department of Biomedical Engineering, Washington University in Saint Louis, 3Department of Ophthalmology and Visual Science, Washington University School of Medicine
This technique describes real time recording of oxygen consumption and extracellular acidification rates in explanted mouse retinal tissues using an extracellular flux analyzer.
