Washington University in Saint Louis

2 ARTICLES PUBLISHED IN JoVE

Biology

Measurement of Energy Metabolism in Explanted Retinal Tissue Using Extracellular Flux Analysis

Jeffrey R. Millman^1,2, Teresa Doggett³, Christina Thebeau³, Sheng Zhang³, Clay F. Semenkovich¹, Rithwick Rajagopal³

¹Division of Metabolism, Endocrinology and Lipid Research, Department of Medicine, Washington University School of Medicine, ²Department of Biomedical Engineering, Washington University in Saint Louis, ³Department of Ophthalmology and Visual Science, Washington University School of Medicine

This technique describes real time recording of oxygen consumption and extracellular acidification rates in explanted mouse retinal tissues using an extracellular flux analyzer.

Engineering

Fabrication of High-Quality Whispering Gallery Mode Microbubble Resonators

Maxwell R. Adolphson¹, Jie Liao¹, Abraham Qavi^1,2, Gaya Amarasinghe³, Lan Yang¹

¹Department of Electrical and Systems Engineering, Washington University in Saint Louis, ²Department of Pathology and Laboratory Medicine, University of California, Irvine, ³Department of Pathology and Immunology, Washington University School of Medicine

Here, we demonstrate a robust and standardized protocol for fabricating high-quality factor (Q-factor) Whispering Gallery Mode (WGM) Microbubble resonators (MBRs) with a precision glass processing machine (PGP).