The Graduate Center of the City University of New York

4 ARTICLES PUBLISHED IN JoVE

Medicine

A "Patient-Like" Orthotopic Syngeneic Mouse Model of Hepatocellular Carcinoma Metastasis

Dibash K. Das^1,2, Victoria Durojaiye¹, Adeodat Ilboudo¹, Michelle K. Naidoo¹, Olorunseun Ogunwobi^1,2

¹Department of Biological Sciences, Hunter College of the City University of New York, ²Departments of Biology and Biochemistry, The Graduate Center of the City University of New York

The metastatic spread of cancer is the major cause of cancer-related deaths. We provide an in-depth description of our survival surgery methodology for establishing a “patient-like” orthotopic syngeneic mouse model system for studying the mechanisms of metastasis in solid organ tumors.

Medicine

Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model

Dibash K. Das^1,2, Michelle K. Naidoo¹, Adeodat Ilboudo¹, Pascal DuBois¹, Victoria Durojaiye¹, Chen Liu³, Olorunseun O. Ogunwobi^1,2

¹Department of Biological Sciences, Hunter College of The City University of New York, ²Departments of Biology and Biochemistry, The Graduate Center of The City University of New York, ³Department of Pathology, Immunology, and Laboratory Medicine, University of Florida

Circulating tumor cells (CTCs) have been shown to play an important role in tumor metastasis. Here, a method for the isolation and propagation of CTCs from the whole blood of a syngeneic mouse tumor model of hepatocellular carcinoma (HCC) metastasis is described.

Cancer Research

Surface-enhanced Resonance Raman Scattering Nanoprobe Ratiometry for Detecting Microscopic Ovarian Cancer via Folate Receptor Targeting

Chrysafis Andreou¹, Anton Oseledchyk¹, Fay Nicolson¹, Naxhije Berisha^1,2, Suchetan Pal¹, Moritz F. Kircher^1,3,4,5,6,7

¹Department of Radiology, Memorial Sloan Kettering Cancer Center, ²Department of Chemistry, The Graduate Center of the City University of New York, ³Molecular Pharmacology Program, Memorial Sloan Kettering Cancer Center, ⁴Center for Molecular Imaging and Nanotechnology (CMINT), Memorial Sloan Kettering Cancer Center, ⁵Gerstner Sloan Kettering Graduate School of Biomedical Sciences, ⁶Department of Radiology, Weill Cornell Medical College of Cornell University, ⁷Dana-Farber Cancer Institute and Harvard Medical Center

Ovarian cancer forms metastases throughout the peritoneal cavity. Here, we present a protocol to make and use folate-receptor targeted surface-enhanced resonance Raman scattering nanoprobes that reveal these lesions with high specificity via ratiometric imaging. The nanoprobes are administered intraperitoneally to living mice, and the derived images correlate well with histology.

Bioengineering

A Tripeptide-Stabilized Nanoemulsion of Oleic Acid

Sylwia A. Dragulska¹, Marek T. Wlodarczyk^1,2, Mina Poursharifi^1,3, John A. Martignetti^4,5,6, Aneta J. Mieszawska^1,2,3

¹Department of Chemistry, Brooklyn College, The City University of New York, ²Ph.D. Program in Chemistry, The Graduate Center of The City University of New York, ³Ph.D. Program in Biochemistry, The Graduate Center of The City University of New York, ⁴Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, ⁵Women's Health Research Institute, Icahn School of Medicine at Mount Sinai, ⁶Laboratory for Translational Research, Western Connecticut Health Network

This protocol describes an efficient method to synthesize a nanoemulsion of an oleic acids-platinum(II) conjugate stabilized with a lysine-tyrosine-phenylalanine (KYF) tripeptide. The nanoemulsion forms under mild synthetic conditions via self-assembly of the KYF and the conjugate.