United Arab Emirates University

3 ARTICLES PUBLISHED IN JoVE

Biology

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Amnah Alzahmi^1,2, Sarah Daakour¹, Diana Charles El Assal³, Bushra S. Dohai³, Amphun Chaiboonchoe³, Weiqi Fu^3,4, David R. Nelson¹, Alexandra Mystikou¹, Kourosh Salehi-Ashtiani^1,3

¹Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi Research Institute, ²Department of Biology, United Arab Emirates University, ³Laboratory of Algal, Systems, and Synthetic Biology (LASSB), Division of Science and Math, New York University Abu Dhabi, ⁴Department of Marine Science, Ocean College, Zhejiang University

This protocol demonstrates the use of a phenotype microarray (PM) technology platform to define metabolic requirements of Chlamydomonas reinhardtii, a green microalga, and refine an existing metabolic network model.

Biology

Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)

Muhammad Abid Sheikh¹, Suraiya Anjum Ansari^1,2

¹Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, ²Zayed Center for Health Sciences, United Arab Emirates University

Using the low-cost cationic polymer polyethylenimine (PEI), we produced lentiviral particles for stable expression of shRNAs in H9 human embryonic stem cells (hESCs) and transiently transduced H9-derived neural progenitor cells (NPCs) at high efficiency.

Biology

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

Muhammad Abid Sheikh¹, Farah Helena Afandi¹, Grazia Iannello², Barbara Corneo², Bright Starling Emerald^3,4,5, Suraiya Anjum Ansari^1,4,5

¹Department of Biochemistry and Molecular Biology, College of Medicine and Health Sciences, United Arab Emirates University, ²Columbia Stem Cell Initiative, Stem Cell Core, Columbia University Irving Medical Center, ³Department of Anatomy, College of Medicine and Health Sciences, United Arab Emirates University, ⁴Zayed Center for Health Sciences, United Arab Emirates University, ⁵ASPIRE Precision Medicine Research Institute Abu Dhabi (PMRI-AD), United Arab Emirates University

The presented method describes the generation of a CRISPR-mediated gene knockout in the human embryonic stem cell (hESC) line H9, which stably expresses sgRNAs targeting the L2HGDH gene using a highly efficient lentiviral-mediated gene delivery system.