Oncode Institute
2 ARTICLES PUBLISHED IN JoVE
Biology
Generation of Human Kidney Tubuloids from Tissue and Urine
Camilla Calandrini 1,2, Jarno Drost 1,2
1Princess Máxima Center for Pediatric Oncology, 2Oncode Institute
Human kidney tubuloid cultures represent a valuable in vitro model to study kidney physiology and disease. Tubuloids can be established from kidney tissue (healthy and diseased) as well as urine, the latter representing an easily obtainable and less invasive source of research material.
Biology
Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling
Dimitrios Laskaris *1,2, Maria Azkanaz *1,2, Mijke A. de Vreij-Kruidenier 3, Doreen van Rijswoud-Ram 3, Hendrik A. Messal 1,2, Jacco van Rheenen 1,2
1Department of Molecular Pathology, The Netherlands Cancer Institute, 2Oncode Institute, 3Animal Laboratory Facility, The Netherlands Cancer Institute
Here, we present a method to obtain images of the intestine upon laser-induced wounding. By exposing the mouse intestine to a multiphoton laser, the loss of a single or multiple crypt(s) is induced locally. By repeatedly imaging the damaged area over months, the real-time dynamics of intestinal recovery is captured.
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