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Oncode Institute

2 ARTICLES PUBLISHED IN JoVE

Biology

Generation of Human Kidney Tubuloids from Tissue and Urine

¹Princess Máxima Center for Pediatric Oncology, ²Oncode Institute

Human kidney tubuloid cultures represent a valuable in vitro model to study kidney physiology and disease. Tubuloids can be established from kidney tissue (healthy and diseased) as well as urine, the latter representing an easily obtainable and less invasive source of research material.

Biology

Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling

Dimitrios Laskaris^*1,2, Maria Azkanaz^*1,2, Mijke A. de Vreij-Kruidenier³, Doreen van Rijswoud-Ram³, Hendrik A. Messal^1,2, Jacco van Rheenen^1,2

¹Department of Molecular Pathology, The Netherlands Cancer Institute, ²Oncode Institute, ³Animal Laboratory Facility, The Netherlands Cancer Institute

Here, we present a method to obtain images of the intestine upon laser-induced wounding. By exposing the mouse intestine to a multiphoton laser, the loss of a single or multiple crypt(s) is induced locally. By repeatedly imaging the damaged area over months, the real-time dynamics of intestinal recovery is captured.