Federal University of Pelotas
2 ARTICLES PUBLISHED IN JoVE
Biology
Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome
Sarah R. Ocañas *1,2,3, José V. V. Isola *1,3, Tatiana D. Saccon 1, Kevin D. Pham 2, Ana J. Chucair-Elliott 2, Augusto Schneider 4, Willard M. Freeman 2,3, Michael B. Stout 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
In this protocol, the translating ribosome affinity purification (TRAP) method and the isolation of nuclei tagged in specific cell types (INTACT) method were optimized for the paired interrogation of the cell-specific ovarian transcriptome and epigenome using the NuTRAP mouse model crossed to a Cyp17a1-Cre mouse line.
Immunology and Infection
Experimental Model of Ligature-Induced Peri-Implantitis in Mice
Davi Neto de Araújo Silva 1, Maísa Casarin 2, Sepehr Monajemzadeh 1, Taciane Menezes da Silveira 2, Jacob Lubben 1, Beatriz Bezerra 1, Flavia Q. Pirih 1
1School of Dentistry, Section of Periodontics, University of California, 2School of Dentistry, Department of Periodontology, Federal University of Pelotas
This is a report on an experimental model of ligature-induced peri-implantitis in mice. We describe all surgical steps, from pre- and post-operative management of the animals, extractions, implant placement, and ligature-induced peri-implantitis.
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