Accedi

Federal University of Pelotas

2 ARTICLES PUBLISHED IN JoVE

Biology

Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome

Sarah R. Ocañas^*1,2,3, José V. V. Isola^*1,3, Tatiana D. Saccon¹, Kevin D. Pham², Ana J. Chucair-Elliott², Augusto Schneider⁴, Willard M. Freeman^2,3, Michael B. Stout^1,3

¹Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, ²Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, ³Oklahoma City Veterans Affairs Medical Center, ⁴Nutrition College, Federal University of Pelotas

In this protocol, the translating ribosome affinity purification (TRAP) method and the isolation of nuclei tagged in specific cell types (INTACT) method were optimized for the paired interrogation of the cell-specific ovarian transcriptome and epigenome using the NuTRAP mouse model crossed to a Cyp17a1-Cre mouse line.

Immunology and Infection

Experimental Model of Ligature-Induced Peri-Implantitis in Mice

Davi Neto de Araújo Silva¹, Maísa Casarin², Sepehr Monajemzadeh¹, Taciane Menezes da Silveira², Jacob Lubben¹, Beatriz Bezerra¹, Flavia Q. Pirih¹

¹School of Dentistry, Section of Periodontics, University of California, ²School of Dentistry, Department of Periodontology, Federal University of Pelotas

This is a report on an experimental model of ligature-induced peri-implantitis in mice. We describe all surgical steps, from pre- and post-operative management of the animals, extractions, implant placement, and ligature-induced peri-implantitis.