July 6th, 2010
•We demonstrate the use of the gene gun to introduce fluorescent dyes, such as DiI, into neurons in brain slices from rodents and non-human primates of different ages. In this particular case, we use adult mice (3-6 months old) and adult cynomologus monkeys (9-15 years old). This technique, originally described by the laboratory of Dr. Lichtman (Gan et al., 2000), is well suited for the study of dendritic branching and dendritic spine morphology and can be combined with traditional immunostaining, if detergents are kept at a low concentration.
Tags
Related Videos
Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices
Growing Neural Stem Cells from Conventional and Nonconventional Regions of the Adult Rodent Brain
Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains
Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
Evaluation of Synapse Density in Hippocampal Rodent Brain Slices
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays