Cell Culture of Feline Astrocytes and Treatment with H2O2
5:08
Staining for Reactive Oxygen Species
6:45
Flow Cytometry
8:18
Representative Flow Cytometric Results
9:05
Conclusion
A screening method to detect oxidative cellular environments is to measure the oxidation of CM-H2DCFDA. Once oxidized within a cell, CM-H2DCFDA changes from non-fluorescent into a fluorescent compound. This change in fluorescence is measured by flow cytometry and indicates the number of cells in an oxidative environment.
