Name: gold nanostar synthesis with a silver seed mediated growth method
Uploaded: 2012-01-15T14:00:00
Description: Watch this Scientific Journal Video about Gold Nanostar Synthesis with a Silver Seed Mediated Growth Method at JoVE.com

This research was supported by the National Science Foundation Partnerships for Research and Education in Materials (PREM) Grant No. DMR-0934218. It was also supported by Award Number 2G12RR013646-11 from the National Center For Research Resources. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center For Research Resources or the National Institutes of Health.

1. Silver seed preparation
<ol>
	<li>Prepare a stock solution of silver nitrate (AgNO3) by taking an arbitrary mass and mixing it with 10 mL of deionized (DI) water. Calculate molarity of the solution. Keep the solution in a dark place to isolate it from light.</li>
	<li>Add 14.7 mg of sodium citrate tribasic (Na3C6H5O7) to 10 mL of DI water to make a 5 mM solution. Shake the vial until the powder is dissolved.</li>
	<li>Add 15.1 mg of sodium borohydride (NaBH...

<ol>
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D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cellular+uptake+and+cytotoxicity+of+gold+nanorods:+molecular+origin+of+cytotoxicity+and+surface+effects.">Cellular uptake and cytotoxicity of gold nanorods: molecular origin of cytotoxicity and surface effects.</a> Small. 5, 701-708 (2009).</li><li>Sun, L., Liu, D., Wang, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+gold+nanoparticle-peptide+complexes+as+cell-targeting+agents.">Functional gold nanoparticle-peptide complexes as cell-targeting agents.</a> Langmuir. 24, 10293-10297 (2008).</li><li>Park, J., Estrada, A., Sharp, K., Sang, K., Schwartz, J. A., Smith, D. K., Coleman, C., Payne, J. D., Korgel, B. A., Dunn, A. K., Tunnell, J. 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In this work we have presented a method to synthesize gold nanostars using silver seeds. We found that silver seeds resulted in a yield of 70 % production of nanostars. The nanostars have a near infrared absorption peak, corresponding to their surface plasmon resonance mode, centered between 800 nm and 850 nm 7, 23. These properties properties allow our gold nanostars to be of use for biomedical applications 24-26, such as photo-thermal ablation. 
A major difference betwe...

<table border="1">
	<tbody>
		<tr>
			<td>Name of the reagent</td>
			<td>Company</td>
			<td>Catalogue number</td>
			<td>Purity</td>
		</tr>
		<tr>
			<td>Sodium citrate tribasic dehydrate</td>
			<td>Sigma</td>
			<td>S4641</td>
			<td>99.0 %</td>
		</tr>
		<tr>
			<td>Silver nitrate</td>
			<td>Aldrich</td>
			<td>204390</td>
			<td>99.9999 %</td>
		</tr>
		<tr>
			<td>Sodium borohydride</td>
			<td>Aldrich</td>
			<td>213462</td>
			<td>99 %</td>
		</tr>
		<tr>
			<td>L-Ascorbic acid</td>
			<td>Sigma-Aldrich</td>
			<td>255564</td>
			<td>99+ %</td>
		</tr>
		<tr>
			<td>Gold chloride trihydrate</td>
			<td>Aldrich</td>
			<td>520918</td>
			<td>99.9+ %</td>
		</tr>
		<tr>
			<td>Hexadecyltrimethylammonium bromide (CTAB)</td>
			<td>Sigma</td>
			<td>H6269</td>
			<td>&nbsp;</td>
		</tr>
	</tbody>
</table>
<table border="1">
	<tbody>
		<tr>
			<td>Name of equipment</td>
			<td>Company</td>
			<td>Comments</td>
		</tr>
		<tr>
			<td>JEOL 2010-F </td>
			<td>JEOL</td>
			<td>Transmission electron microscope</td>
		</tr>
		<tr>
			<td>Hitachi S-5500 </td>
			<td>Hitachi</td>
			<td>Used in scanning electron microscope mode</td>
		</tr>
		<tr>
			<td>Olis Cary-14 spectrophotometer</td>
			<td>Olis</td>
			<td>Spectrophotometer</td>
		</tr>
	</tbody>
</table>

gold nanostar synthesis with a silver seed mediated growth method

The physical, chemical and optical properties of nano-scale colloids depend on their material composition, size and shape 1-5. There is a great interest in using nano-colloids for photo-thermal ablation, drug delivery and many other biomedical applications 6. Gold is particularly used because of its low toxicity 7-9. A property of metal nano-colloids is that they can have a strong surface plasmon resonance 10. The peak of the surface plasmon resonance mode depends on the structure and composition of the metal nano-colloids. Since the surface plasmon resonance mode is stimulated with light there is a need to have the peak absorbance in the near infrared where biological tissue transmissivity is maximal 11, 12.
We present a method to synthesize star shaped colloidal gold, also known as star shaped nanoparticles 13-15 or nanostars 16. This method is based on a solution containing silver seeds that are used as the nucleating agent for anisotropic growth of gold colloids 17-22. Scanning electron microscopy (SEM) analysis of the resulting gold colloid showed that 70 % of the nanostructures were nanostars. The other 30 % of the particles were amorphous clusters of decahedra and rhomboids. The absorbance peak of the nanostars was detected to be in the near infrared (840 nm). Thus, our method produces gold nanostars suitable for biomedical applications, particularly for photo-thermal ablation.

Watch this Scientific Journal Video about Gold Nanostar Synthesis with a Silver Seed Mediated Growth Method at JoVE.com

Gold Nanostar Synthesis with a Silver Seed Mediated Growth Method

We synthesized star shaped gold nanostars using a silver seed mediated growth method. The diameter of the nanostars ranges from 200 to 300 nm and the number of tips vary from 7 to 10. The nanoparticles have a broad surface plasmon resonance mode centered in the near infrared.

The physical, chemical and optical properties of nano-scale colloids depend on their material composition, size and shape 1-5. There is a great interest ...

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