这项研究得到了国家科学基金会为材料的研究和教育伙伴关系（PREM）批准号：DMR - 0934218。它也支持奖号码为国家研究资源中心2G12RR013646 - 11。内容完全是作者的责任，并不代表官方意见的国家研究资源或国家卫生研究院中心。

1。银种子准备<ol><li>准备通过利用一个任意的质量和混合10毫升的去离子（DI）水的原液的硝酸银 （硝酸银）。计算的解决方案的摩尔。保持在一个黑暗的地方隔离开光的解决方案。 </li><li>添加柠檬酸钠三元14.7毫克（钠3 C 6 H 5 O 7）10毫升去离子水，使一个5毫米的解决方案。摇动小瓶直至粉末溶解。 </li><li> 10毫升去离子水15.1毫克的硼氢化钠（硼氢化钠 4）添加到另一个小瓶，使40毫米的解决方案。立即关闭小瓶。用手轻轻摇动的解决方案，并放置在一个冰的烧杯。放置在冰箱的烧杯中，并启动一个定时器（T1 = 0）。新鲜制作的解决方案将用于在15分钟这是有足够的时间冷却下来。 </li><li>从硝酸银，1.1）的股票解决方案，准备在0.25毫米10毫升。放置一个小瓶和ST搅拌磁铁艺术搅拌。 </li><li>加入0.25毫升柠檬酸钠三元解决方案1.2）1.4）。 </li><li>在t 1 = 15分钟去除硼氢化钠溶液，1.3），从冰箱中。用吸管取0.4毫升这一解决方案，并把它添加到1.5）。注：在一个单一的快速中风解决方案。颜色会变成黄色。搅拌5分钟的解决方案。 </li><li>在t 1 = 20分钟停止搅拌，取出小瓶磁铁和小瓶保持在黑暗的地方。不要关闭小瓶。 </li><li>使用前在室温避光保持至少2个小时的解决方案。最好使用一个星期内从准备的种子。 </li></ol> 2。生长溶液的制备<ol><li>准备140毫克，加入10毫升去离子水抗坏血酸（C 6 H 8 O 6）80毫米。 </li><li>准备10毫升的氯化金（HAuCl 4）浓缩液。计算解决方案摩尔。保持的solutioñ从光隔离。 </li><li>准备50毫米十六烷基三甲基溴化铵（CTAB法- C 19 H 42 BRN）20毫升，加入20毫升去离子水364毫克一小瓶。立即放入小瓶一种震撼人心的磁铁，并开始搅拌在一个温暖的板30 ° C。后CTAB的粉末完全溶解，溶液变成透明关闭该板块的热水器，但不断搅拌至步骤2.7）。 </li><li>添加解决方案1.1）的解决方案2.3），获得最终摩尔4.9x10 -2毫米。启动一个定时器（T 2 = 0）。 </li><li>在t 2 = 1分钟将解决方案添加2.2）2.4），获得了0.25毫米的最后摩尔。 </li><li>在T 2 = 2分加0.1毫升）2.1〜2.5）。该解决方案将变成无色。 </li><li>在t 2 = 2分20秒增加0.05毫升1.8）（银种子）为2.6）。搅拌15分钟的暂停。受最初将变成蓝色，然后棕色。 </li><li>在t 2 = 17分钟停止搅拌，取出米agnet和室温保持在24小时暂停。 </li></ol> 3。 CTAB的分离鉴定或实验成像，黄金nanostars 注：CTAB法可在室温下结晶。解散了金胶体晶体加热至30 ° C，或沉浸在热自来水的小瓶，直到晶体溶解。 <ol><li>超声暂停2分钟。 </li><li>离心5分钟730 RCF暂停。 Nanostars将堆积在管壁。 </li><li>删除尽可能多的暂停与照顾不删除的nanostars吸管。 </li><li>加入DI水管和超声2分钟。 </li><li> 3分钟460 RCF离心暂停。暂停含有CTAB的少，因此较低的离心力需要单独的nanostars。 </li><li>重复步骤3.3）和3.4）。 </li><li>加入去离子水380 RCF 3分钟的暂停和离心机。 </li><li> Repea<html>吨步骤3.3）和3.4）。 nanostars成像光谱仪，或实验。 </li></ol> 4。代表性的成果： 图1显示了透射电子显微镜的银种子成像使用的JEOL 2010 - F透射电镜（TEM）图像。种子有球形和15纳米的平均规模。黄金nanostars成像使用一台日立的S - 5500扫描电子显微镜（SEM）模式。图2显示了我们的方法合成的nanostars增加放大倍率。星形颗粒约70％的胶体颗粒。非形成的恒星看起来像decahedra和菱形的无定形簇（未显示）。图3显示了几个单一的黄金nanostars。 nanostars范围从200 nm至300 nm和提示的大小不等的7至10。如果他们保留它们的形状合成后的1个月至少这是留在CTAB法合成纳米金。 e_content“我们测量使用OLIS卡里- 14分光光度计的银种子和nanostars吸收光谱吸收峰在400 nm的种子，而吸收峰800 nm和850 nm处（图4之间的nanostars ）。 <img src="/files/ftp_upload/3570/3570fig1.jpg" alt="图1" /> 图1银种子透射电子显微镜图像。 <img src="/files/ftp_upload/3570/3570fig2.jpg" alt="图2" /> 图2：黄金nanostars扫描电子显微镜图像。 <img src="/files/ftp_upload/3570/3570fig3.jpg" alt="图3" /> 图3：单金nanostars扫描电子显微镜图像。 <img src="/files/ftp_upload/3570/3570fig4.jpg" alt="图6" /> 图4银种子规范化的吸收光谱（虚线）和金nanostars（实线）。

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没有利益冲突的声明。

在这项工作中，我们提出了一个方法来合成黄金nanostars使用银种子。我们发现，银种子产量的70％nanostars生产。 nanostars近红外吸收峰，对应其表面等离子体共振模式，800纳米和850纳米，23间为中心的。这些物业允许使用医学领域的应用24日至26日 ，如光热消融，我们的黄金nanostars。 一个方法之间的主要区别在这里所说的其他方法是使用，而不是黄金白银种子。...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td><td> 纯度 </td></tr><tr><td>柠檬酸钠三元脱水</td><td>西格玛</td><td> S4641 </td><td> 99.0％ </td></tr><tr><td>硝酸银</td><td>爱秩序</td><td> 204390 </td><td> 99.9999％ </td></tr><tr><td>硼氢化钠</td><td>爱秩序</td><td> 213462 </td><td> 99％ </td></tr><tr><td> L -抗坏血酸</td><td> Sigma - Aldrich公司</td><td> 255564 </td><td> 99％ </td></tr><tr><td>黄金氯化三水</td><td>爱秩序</td><td> 520918 </td><td> 99.9％以上</td></tr><tr><td>十六溴铵（CTAB） </td><td>西格玛</td><td> H6269 </td><td></td></tr></tbody></table><table border="1"><tbody><tr><td> 设备名称 </td><td> 公司 </st荣&gt; </td><td> 评论 </td></tr><tr><td> JEOL 2010 - F </td><td> JEOL </td><td>透射电子显微镜</td></tr><tr><td>日立S - 5500 </td><td>日立</td><td>使用扫描电子显微镜模式</td></tr><tr><td> OLIS卡里- 14分光光度计</td><td> OLIS </td><td>分光光度计</td></tr></tbody></table>

gold nanostar synthesis with a silver seed mediated growth method

纳米级胶体的物理，化学和光学特性取决于他们的物质组成，大小和形状1-5。有极大的兴趣，在光热消融，药物输送和许多其他医学领域的应用中使用的纳米胶体。黄金特别是因为它的低毒性 7-9 。金属纳米胶体的财产是他们能有一个强有力的表面等离子体共振 10 。表面等离子体共振模式的峰值取决于金属纳米胶体的结构和组成。由于表面等离子体共振模式是与光的刺激，有必要在近红外生物组织透射率是最大的 11，12峰吸光度。 我们提出了一个方法来合成星形胶体金，又称星形13-15纳米粒子或nanostars 16。这种方法是基于作为olution含银种子是用来作为核剂的胶体金17-22各向异性增长。扫描电子显微镜（SEM）分析所产生的金胶体的结果显示，70％的纳米结构nanostars。其余30％的颗粒无定形集群decahedra和菱形。在近红外（840 nm）的吸收峰的nanostars检测。因此，我们的方法生产的黄金nanostars适用于生物医学应用，特别是光热消融。

Watch this Scientific Journal Video about Gold Nanostar Synthesis with a Silver Seed Mediated Growth Method at JoVE.com

Gold Nanostar Synthesis with a Silver Seed Mediated Growth Method

我们合成了星形黄金nanostars使用银种子介导的增长方式。 nanostars直径范围从200到300 nm，并提示7日至10不等。纳米粒子具有广泛的表面等离子体共振模式在近红外的中心。

黄金Nanostar合成银种子介导的生长方法

The physical, chemical and optical properties of nano-scale colloids depend on their material composition, size and shape 1-5. There is a great interest ...

gold-nanostar-synthesis-with-a-silver-seed-mediated-growth-method

Research

JoVE Journal

Bioengineering

24.9K 次观看.  The University of Texas at San Antonio. 我们合成了星形黄金nanostars使用银种子介导的增长方式。 nanostars直径范围从200到300 nm，并提示7日至10不等。纳米粒子具有广泛的表面等离子体共振模式在近红外的中心。

视频: Gold Nanostar Synthesis with a Silver Seed Mediated Growth Method

Axon Stretch Growth: The Mechanotransduction of Neuronal Growth

During pre-synaptic embryonic development, neuronal processes traverse short distances to reach their targets via growth cone. Over time, neuronal somata are separated from their axon terminals due to skeletal growth of the enlarging organism (Weiss 1941; Gray, Hukkanen et al. 1992). This mechanotransduction induces a secondary mode of neuronal growth capable of accommodating continual elongation of the axon (Bray 1984; Heidemann and Buxbaum 1994; Heidemann, Lamoureux et al. 1995; Pfister, Iwata et al. 2004).
Axon Stretch Growth (ASG) is conceivably a central factor in the maturation of short embryonic processes into the long nerves and white matter tracts characteristic of the adult nervous system. To study ASG in vitro, we engineered bioreactors to apply tension to the short axonal processes of neuronal cultures (Loverde, Ozoka et al. 2011). Here, we detail the methods we use to prepare bioreactors and conduct ASG. First, within each stretching lane of the bioreactor, neurons are plated upon a micro-manipulated towing substrate. Next, neurons regenerate their axonal processes, via growth cone extension, onto a stationary substrate. Finally, stretch growth is performed by towing the plated cell bodies away from the axon terminals adhered to the stationary substrate; recapitulating skeletal growth after growth cone extension.
Previous work has shown that ASG of embryonic rat dorsal root ganglia neurons are capable of unprecedented growth rates up to 10mm/day, reaching lengths of up to 10cm; while concurrently resulting in increased axonal diameters (Smith, Wolf et al. 2001; Pfister, Iwata et al. 2004; Pfister, Bonislawski et al. 2006; Pfister, Iwata et al. 2006; Smith 2009). This is in dramatic contrast to regenerative growth cone extension (in absence of mechanical stimuli) where growth rates average 1mm/day with successful regeneration limited to lengths of less than 3cm (Fu and Gordon 1997; Pfister, Gordon et al. 2011). Accordingly, further study of ASG may help to reveal dysregulated growth mechanisms that limit regeneration in the absence of mechanical stimuli.

A unique tissue engineering method was developed to elongate numerous nerve fibers in culture by recapitulating axon stretch growth; a form of nervous system growth whereby nerves elongate in conjunction with growth of the enlarging body.

轴突伸展生长：神经生长Mechanotransduction

During pre-synaptic embryonic development, neuronal processes traverse short distances to reach their targets via growth cone. Over time, neuronal somata ...

科研

生物工程

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Colloidal gold nanoparticles protected with alkanethiolate ligands called monolayer protected gold clusters (MPCs) are synthesized and subsequently incorporated into film assemblies that serve as adsorption platforms for protein monolayer electrochemistry (PME). PME is utilized as the model system for studying electrochemical properties of redox proteins by confining them to an adsorption platform at a modified electrode, which also serves as a redox partner for electron transfer (ET) reactions. Studies have shown that gold nanoparticle film assemblies of this nature provide for a more homogeneous protein adsorption environment and promote ET without distance dependence compared to the more traditional systems modified with alkanethiol self-assembled monolayers (SAM).1-3 In this paper, MPCs functionalized with hexanethiolate ligands are synthesized using a modified Brust reaction4 and characterized with ultraviolet visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), and proton (1H) nuclear magnetic resonance (NMR). MPC films are assembled on SAM modified gold electrode interfaces by using a "dip cycle" method of alternating MPC layers and dithiol linking molecules. Film growth at gold electrode is tracked electrochemically by measuring changes to the double layer charging current of the system. Analogous films assembled on silane modified glass slides allow for optical monitoring of film growth and cross-sectional TEM analysis provides an estimated film thickness. During film assembly, manipulation of the MPC ligand protection as well as the interparticle linkage mechanism allow for networked films, that are readily adaptable, to interface with redox protein having different adsorption mechanism. For example, Pseudomonas aeruginosa azurin (AZ) can be adsorbed hydrophobically to dithiol-linked films of hexanethiolate MPCs and cytochrome c (cyt c) can be immobilized electrostatically at a carboxylic acid modified MPC interfacial layer. In this report, we focus on the film protocol for the AZ system exclusively. Investigations involving the adsorption of proteins on MPC modified synthetic platforms could further the understanding of interactions between biomolecules and man-made materials, and consequently aid the development of biosensor schemes, ET modeling systems, and synthetic biocompatible materials.5-8

Alkanethiolate stabilized gold colloids known as monolayer protected clusters (MPCs) are synthesized, characterized, and assembled into thin films as an adsorption interface for protein monolayer electrochemistry of simple redox protein like Pseudomonas aeruginosa azurin (AZ) and cytochrome c (cyt c).

单层的合成，装配和表征蛋白质单分子膜的电化学保护的金纳米粒子薄膜

Colloidal gold nanoparticles protected with alkanethiolate ligands called monolayer protected gold clusters (MPCs) are synthesized and subsequently ...

Observing and Quantifying Fibroblast-mediated Fibrin Gel Compaction

Cells embedded in collagen and fibrin gels attach and exert traction forces on the fibers of the gel. These forces can lead to local and global reorganization and realignment of the gel microstructure. This process proceeds in a complex manner that is dependent in part on the interplay between the location of the cells, the geometry of the gel, and the mechanical constraints on the gel. To better understand how these variables produce global fiber alignment patterns, we use time-lapse differential interference contrast (DIC) microscopy coupled with an environmentally controlled bioreactor to observe the compaction process between geometrically spaced explants (clusters of fibroblasts). The images are then analyzed with a custom image processing algorithm to obtain maps of the strain. The information obtained from this technique can be used to probe the mechanobiology of various cell-matrix interactions, which has important implications for understanding processes in wound healing, disease development, and tissue engineering applications.

Time-lapse microscopy and image processing techniques were used to observe and analyze fibroblast-mediated gel compaction and fibrin fiber realignment in an environmentally controlled bioreactor over a 48 hr&#160;period.

观察和量化纤维细胞介导的纤维素凝胶压实

Cells embedded in collagen and fibrin gels attach and exert traction forces on the fibers of the gel. These forces can lead to local and global ...

Synthesis of an Intein-mediated Artificial Protein Hydrogel

We present the synthesis of a highly stable protein hydrogel mediated by a split-intein-catalyzed protein trans-splicing reaction. The building blocks of this hydrogel are two protein block-copolymers each containing a subunit of a trimeric protein that serves as a crosslinker and one half of a split intein. A highly hydrophilic random coil is inserted into one of the block-copolymers for water retention. Mixing of the two protein block copolymers triggers an intein trans-splicing reaction, yielding a polypeptide unit with crosslinkers at either end that rapidly self-assembles into a hydrogel. This hydrogel is very stable under both acidic and basic conditions, at temperatures up to 50 &#176;C, and in organic solvents. The hydrogel rapidly reforms after shear-induced rupture. Incorporation of a &#34;docking station peptide&#34; into the hydrogel building block enables convenient incorporation of &#34;docking protein&#34;-tagged target proteins. The hydrogel is compatible with tissue culture growth media, supports the diffusion of 20 kDa molecules, and enables the immobilization of bioactive globular proteins. The application of the intein-mediated protein hydrogel as an organic-solvent-compatible biocatalyst was demonstrated by encapsulating the horseradish peroxidase enzyme and corroborating its activity.

We present the synthesis of a split-intein-mediated protein hydrogel. The building blocks of this hydrogel are two protein copolymers each containing a subunit of a trimeric protein that serves as a crosslinker and one half of a split intein. Mixing of the two protein copolymers triggers an intein trans-splicing reaction, yielding a polypeptide unit that self-assembles into a hydrogel. This hydrogel is highly pH- and temperature-stable, compatible with organic solvents, and easily incorporates functional globular proteins.

内含肽介导的人工蛋白水凝胶的合成

We present the synthesis of a highly stable protein hydrogel mediated by a split-intein-catalyzed protein trans-splicing reaction. The building blocks of ...

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Lipid bilayer membranes form the plasma membranes of cells and define the boundaries of subcellular organelles. In nature, these membranes are heterogeneous mixtures of many types of lipids, contain membrane-bound proteins and are decorated with carbohydrates. In some experiments, it is desirable to decouple the biophysical or biochemical properties of the lipid bilayer from those of the natural membrane. Such cases call for the use of model systems such as giant vesicles, liposomes or supported lipid bilayers (SLBs). Arrays of SLBs are particularly attractive for sensing applications and mimicking cell-cell interactions. Here we describe a new method for forming SLB arrays. Submicron-diameter SiO2 beads are first coated with lipid bilayers to form spherical SLBs (SSLBs). The beads are then deposited into an array of micro-fabricated submicron-diameter microwells. The preparation technique uses a "squeegee" to clean the substrate surface, while leaving behind SSLBs that have settled into microwells. This method requires no chemical modification of the microwell substrate, nor any particular targeting ligands on the SSLB. Microwells are occupied by single beads because the well diameter is tuned to be just larger than the bead diameter. Typically, more 75% of the wells are occupied, while the rest remain empty. In buffer SSLB arrays display long-term stability of greater than one week. Multiple types of SSLBs can be placed in a single array by serial deposition, and the arrays can be used for sensing, which we demonstrate by characterizing the interaction of cholera toxin with ganglioside GM1. We also show that phospholipid vesicles without the bead supports and biomembranes from cellular sources can be arrayed with the same method and cell-specific membrane lipids can be identified.

Supported lipid bilayers and natural membrane particles are convenient systems that can approximate the properties of cell membranes and be incorporated in a variety of analytical strategies. Here we demonstrate a method for preparing microarrays composed of supported lipid bilayer-coated SiO2 beads, phospholipid vesicles or natural membrane particles.

生物膜的微阵列用刮墨刀型组合方式形成

Lipid bilayer membranes form the plasma membranes of cells and define the boundaries of subcellular organelles. In nature, these membranes are ...

Synthesis of Keratin-based Nanofiber for Biomedical Engineering

Electrospinning, due to its versatility and potential for applications in various fields, is being frequently used to fabricate nanofibers. Production of these porous nanofibers is of great interest due to their unique physiochemical properties. Here we elaborate on the fabrication of keratin containing poly (&#949;-caprolactone) (PCL) nanofibers (i.e., PCL/keratin composite fiber). Water soluble keratin was first extracted from human hair and mixed with PCL in different ratios. The blended solution of PCL/keratin was transformed into nanofibrous membranes using a laboratory designed electrospinning set up. Fiber morphology and mechanical properties of the obtained nanofiber were observed and measured using scanning electron microscopy and tensile tester. Furthermore, degradability and chemical properties of the nanofiber were studied by FTIR. SEM images showed uniform surface morphology for PCL/keratin fibers of different compositions. These PCL/keratin fibers also showed excellent mechanical properties such as Young&#39;s modulus and failure point. Fibroblast cells were able to attach and proliferate thus proving good cell viability. Based on the characteristics discussed above, we can strongly argue that the blended nanofibers of natural and synthetic polymers can represent an excellent development of composite materials that can be used for different biomedical applications.

Electrospun nanofibers have a high surface area to weight ratio, excellent mechanical integrity, and support cell growth and proliferation. These nanofibers have a wide range of biomedical applications. Here we fabricate keratin/ PCL nanofibers, using the electrospinning technique, and characterize the fibers for possible applications in tissue engineering.

基于角蛋白纳米纤维生物医学工程合成

Electrospinning, due to its versatility and potential for applications in various fields, is being frequently used to fabricate nanofibers. Production of ...

Synthesis of Gold Nanoparticle Integrated Photo-responsive Liposomes and Measurement of Their Microbubble Cavitation upon Pulse Laser Excitation

Photo-responsive nanoparticles (NPs) have received considerable attention because of their potential in providing spatial, temporal, and dosage control over the drug release. However, most of the relevant technologies are still in the development process and are unprocurable by clinics. Here, we describe a facile fabrication of these photo-responsive NPs with commercially available gold NPs and thermo-responsive liposomes. Calcein is used as a model drug to evaluate the encapsulation efficiency and the release kinetic profile upon heat/light stimulation. Finally, we show that this photo-triggered release is due to the membrane disruption caused by microbubble cavitation, which can be measured with hydrophone.

This protocol describes a simple preparation method for gold nanoparticle integrated photo-responsive liposomes with the commercially available materials. It also shows how to measure the microbubble cavitation process of the synthesized liposomes upon the treatment of pulsed laser.

在脉冲激光激发金纳米粒子集成光电响应脂质体及其微泡空化的测量的合成

Photo-responsive nanoparticles (NPs) have received considerable attention because of their potential in providing spatial, temporal, and dosage control ...

Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification

Hydrogels have been widely utilized to enhance the surface hydrophilicity of membranes for water purification, increasing the antifouling properties and thus achieving stable water permeability through membranes over time. Here, we report a facile method to prepare hydrogels based on zwitterions for membrane applications. Freestanding films can be prepared from sulfobetaine methacrylate (SBMA) with a crosslinker of poly(ethylene glycol) diacrylate (PEGDA) via photopolymerization. The hydrogels can also be prepared by impregnation into hydrophobic porous supports to enhance the mechanical strength. These films can be characterized by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) to determine the degree of conversion of the (meth)acrylate groups, using goniometers for hydrophilicity and differential scanning calorimetry (DSC) for polymer chain dynamics. We also report protocols to determine the water permeability in dead-end filtration systems and the effect of foulants (bovine serum albumin, BSA) on membrane performance.

This paper reports practical methods to prepare hydrogels in freestanding films and impregnated membranes and to characterize their physical properties, including water transport properties.

水凝胶的合成与防污性能作为膜的净水

Hydrogels have been widely utilized to enhance the surface hydrophilicity of membranes for water purification, increasing the antifouling properties and ...

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Gold nanoparticles (AuNPs) have been used extensively in medical research due to their size, biocompatibility, and modifiable surface. Specific targeting and drug delivery are some of the applications of these AuNPs, but endothelial extracellular matrices&#39; defensive properties hamper particle uptake. To address this issue, we describe a synthesis method for ultrasmall gold nanoparticles to improve vascular delivery, with customizable functional groups and polymer lengths for further adjustments. The protocol yields 2.5 nm AuNPs that are capped with tetrakis(hydroxymethyl)phosphonium chloride (THPC). The replacement of THPC with hetero-functional polyethylene glycol (PEG) on the surface of the AuNP increases the hydrodynamic radius to 10.5 nm while providing various functional groups on the surface. The last part of the protocol includes an optional addition of a fluorophore to allow the AuNPs to be visualized under fluorescence to track nanoparticle uptake. Dialysis and lyophilization were used to purify and isolate the AuNPs. These fluorescent nanoparticles can be visualized in both in vitro and in vivo experiments due to the biocompatible PEG coating and fluorescent probes. Additionally, the size range of these nanoparticles render them an ideal candidate for probing the glycocalyx without disrupting normal vasculature function, which may lead to improved delivery and therapeutics.

We describe a method of synthesizing biocompatible 10-nm gold nanoparticles, functionalized by coating poly-ethylene glycol onto the surface. These particles can be used in vitro and in vivo for delivering therapeutics to nanoscale cellular and extracellular spaces that are difficult to access with conventional nanoparticle sizes.

用于内皮靶向和药物传递的功能化10纳米高分子涂层金粒子的合成

Gold nanoparticles (AuNPs) have been used extensively in medical research due to their size, biocompatibility, and modifiable surface. Specific targeting ...

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties

In this study, multi-walled carbon nanotubes (MWCNTs) were treated with an aqueous sulfuric acid solution to form an oxygen-based functional group. Silver MWCNTs were prepared by the reductive deposition of silver from an aqueous solution of AgNO3 on the oxidized MWCNTs. Given the unique color of the CNTs, it was not possible to apply them to the minimum inhibitory concentration or mitochondrial toxicity assays to evaluate the toxicity and antibacterial properties, since they would interfere with the assays. The inhibition zone and minimum bactericidal concentration for the Ag-MWCNTs were measured and Live/Dead and Trypan Blue assays were used to measure the toxicity and antibacterial properties without interfering with the color of the CNTs.

In this study, antimicrobial nanomaterials were synthesized by acidic oxidation of multiwalled carbon nanotubes and subsequent reductive deposition of silver nanoparticles. Antimicrobial activity and cytotoxicity tests were performed with the as-prepared nanomaterials.

银纳米粒子改性多壁碳纳米管的合成及其抗菌活性和细胞毒性的评价

In this study, multi-walled carbon nanotubes (MWCNTs) were treated with an aqueous sulfuric acid solution to form an oxygen-based functional group. Silver ...