Generation of Organotypic Raft Cultures from Primary Human Keratinocytes
February 22nd, 2012
•An in vitro method to mimic in vivo epithelial differentiation is described. Many viruses target epithelial cells as part of their viral life cycle, and this method provides a means of examining virus:host interactions that more closely resembles that which occurs in vivo. This technique can be used with primary keratinocytes, established cell lines, as well as normal or diseased biopsy tissue.
Tags
Related Videos
Generation of Recombinant Influenza Virus from Plasmid DNA
A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue
Generation of Human Alloantigen-specific T Cells from Peripheral Blood
Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
Preparation and Applications of Organotypic Thymic Slice Cultures
Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
