Isolation of Dry Algal Biomass to be Used as Standards for Fluorescence Readings
2:52
Gravimetric Quantification of Neutral Lipids by Hexane Extraction
4:48
Fluorometric Quantification of Neutral Lipids Using Nile Red
6:42
Fluorescence Microscopy Technique
8:04
Results: Images and Calibration Curves of A. protothecoides Stained with Nile Red
10:18
Conclusion
A simple protocol to determine the neutral lipid content of algal cells using a Nile Red staining procedure is described. This time-saving technique offers an alternative to traditional gravimetric-based lipid quantification protocols. It has been designed for the specific application of monitoring bioprocess performance.
