Stabilizing, Dehydrating and Hybridization of Spreads with L1-labeled Retrotransposition Probes
7:26
Washing or Addition of Secondary Antibody and Viewing
8:49
Results: Long Interspersed Nuclear Element-1, or LINE-1 Retrotransposition Analysis
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Conclusion
副本
The overall goal of this procedure is to visualize and analyze L-1 retrotransposition rates and patterns at the single nucleus level using fluorescence in situ hybridization, or FISH. The major advantage of this methodology is the fact that it all
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Here we employ FISH methodology to track LINE-1 retrotransposition at the single nuclei level in chromosome spreads of HepG2 cell lines stably expressing synthetic LINE-1.
