Breeding tsMARCM-ready Flies to Generate tsMARCM Clones
2:25
Preparation, Staining, and Mounting of Fly Brains Containing tsMARCM Clones
4:35
Results: Neural Lineage Analysis Using Four tsMARCM Clones
5:55
Conclusion
Transcript
The overall goal of this twin-spot MARCM protocol is to conduct high resolution cell lineage analyses and better gene function studies. Brain tissue is comprised of a vast number of diverse neurons, it's challenging to study the mechanism underlyi
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Here, we present a protocol for a mosaic labeling technique that permits the visualization of neurons derived from a common progenitor cell in two distinct colors. This facilitates neural lineage analysis with the capability of birth-dating individual neurons and studying gene function in the same neurons of different individuals.
