Preparation of Elastomeric Stamps for 3D Micropatterning
2:48
Preparation of 3D Epithelial Tissues
6:58
Results: 3D Culture System Provides Insight to Growth Factor Mediated Branching Morphogenesis
7:58
Conclusion
Transcript
The goal of this procedure is to engineer a raise of identical three-dimensional tissues that are embedded in a collagen gel for use in many experimental contexts, such as determining the influence of mechanical stress on branching morphogenesis.
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This manuscript describes a soft lithography-based technique to engineer uniform arrays of three-dimensional (3D) epithelial tissues of defined geometry surrounded by extracellular matrix. This method is amenable to a wide variety of cell types and experimental contexts and allows for high-throughput screening of identical replicates.
