July 6th, 2017
•We present a filtration-based protocol to isolate high-quality nuclei from cross-linked mouse skeletal muscle wherein we removed the need for ultracentrifugation, making it easily applicable. We show that chromatin prepared from the nuclei is suitable for chromatin immunoprecipitation and likely chromatin immunoprecipitation sequencing studies.
Related Videos
A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle
Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
Preparation of High-Quality Fermented Fish Product
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria
Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy