Preparation of Recombinant Virus Suitable for Large Scale Expression of IKK1/α
3:41
Purification of His-IKK1
5:54
Screen for Crystallization Condition of IKK1
6:47
Growing Crystals in Large Numbers
7:45
Cryo Protection of Crystals
8:57
Results: Crystallization, and Structure Determination of Human IKK1/α
10:34
Conclusion
Transcript
The goal of this procedure is to provide guidance for the production, crystallization and structural determination of human IKK1-alpha in order to understand the mechanistic basis of its signaling function and to establish platforms for rational d
Sign in or start your free trial to access this content
IκB Kinase 1/α (IKK1/α CHUK) is a Ser/Thr protein kinase that is involved in a myriad of cellular activities primarily through activation of NF-κB transcription factors. Here, we describe the main steps necessary for the production and crystal structure determination of this protein.
