The overall goal of this procedure is to produce in vivo like astrocyte monocultures using a simple, fast, and economical method. This method can help answer key questions in the astroglibiology field, such as what are the economics of astrocyte p
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The AWESAM protocol described here is optimal for culturing murine astrocytes in isolation from other brain cells in a fast, simple, and inexpensive manner. AWESAM astrocytes exhibit spontaneous Ca2+ signaling, morphology, and gene expression profiles similar to astrocytes in vivo.