Pairwise Cross Sibling Heterozygous and Embryo Rearing
4:13
Alcian Blue/Alizarin Red Cartilage and Bone Staining
5:42
Phenotype Scoring and Family Selection
6:49
Results: Selective Breeding for Skeletal Penetrance in Zebrafish
7:58
Conclusion
副本
The overall goal of this breeding strategy is to manipulate mutant penetrance upward or downward. This selective breeding method enables a more complete understanding of mutant phenotypes. By generating high and low penetrance mutant strains, the
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The goal of this protocol is to alter the penetrance of lethal skeletal mutant phenotypes in zebrafish by selective breeding. Lethal mutants cannot be grown to adulthood and bred themselves, therefore this protocol describes a method for tracking and selecting penetrance through multiple generations by progeny testing.
