Cardiac Crescent Stage Embryo Harvest and Processing
2:45
Immunofluorescence Staining
3:57
Embryo Mounting and Confocal Imaging
5:28
Image Analysis and Quantitative 3D Modeling
7:49
Results: Representative Confocal Image Processing
8:56
Conclusion
副本
The overall goal of this method is to quantitatively assess cardiac progenitor cell localization and distribution during heart organogenesis. This method can help answer key answers in the heart development field by allowing us to observe the loca
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Here, we describe a protocol for whole mount immunofluorescence and image-based quantitative volumetric analysis of early stage mouse embryos. We present this technique as a powerful approach to qualitatively and quantitatively assess cardiac structures during development, and propose that it may be widely adaptable to other organ systems.