Dissection of Sciatic Nerve and Dorsal Root Ganglion
4:02
Digestion of Sciatic Nerve and Dorsal Root Ganglion
6:36
Setup and Flow Cytometry and Running of Samples
10:00
Results: Flow Cytometry Analysis of Surface Expressed Markers on Singlets from the Sciatic Nerve and Dorsal Root Ganglion
11:46
Conclusion
副本
The overall goal of this procedure is to obtain enough cells from dorsal root ganglions and sciatic nerves from an individual mouse so as to be able to perform flow cytometry analysis of the cells. Generally, individuals new to this method will st
登录或开始您的免费试用以访问此内容
Quantitative analysis of cell content within the murine sciatic nerve is difficult due to the scarcity of the tissue. This protocol describes a method for tissue digestion and preparation that provides sufficient cells for flow cytometry analysis of immune cell populations from nerves of individual mice.