Seed the hPSCs and Initiate Differentiation to Definitive Endoderm
3:57
Induce the Differentiation into Definitive Endoderm
5:13
Induce the Differentiation into the Primitive Gut Tube
5:47
Induce the Differentiation into PDX1+ Cells
6:28
Results: Analysis of hPSCs and the Generated Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors
8:06
Conclusion
副本
The main advantage of this technique is that before stimulation, cells are dispersed evenly and then stimulated evenly in tandem cell adhesion. Demonstrating the procedure will be Azuma Kimura, a graduate student from our laboratory. To begin this
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Here, we present a detailed protocol to differentiate human pluripotent stem cells (hPSCs) into pancreas/duodenum homeobox protein 1+ (PDX1+) cells for the generation of pancreatic lineages based on the non-colony type monolayer growth of dissociated single cells. This method is suitable for producing homogenous hPSC-derived cells, genetic manipulation and screening.