Results: Expression and Purification of a Mammalian Bestrophin Protein
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Conclusion
副本
This method can help answer key questions related to the biophysics of mammalian ion channels including but not limited to the bestrophin family of ion channels. The main advantage of this technique is that it can be applied to other types of ion
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The purification of ion channels is often challenging, but once achieved, it can potentially allow in vitro investigations of the functions and structures of the channels. Here, we describe the stepwise procedures for the expression and purification of mammalian bestrophin proteins, a family of Ca2+-activated Cl- channels.
