The authors acknowledge funding from National Natural Science Foundation of China (No. 81771971), Shanghai Pujiang Program (No. 14PJD008 and 17PJ1401500), &#34;Chen Guang&#34; Project Supported by Shanghai Municipal Education Commission and Shanghai Education Development Foundation (No. 14CG06), Natural Science Foundation of Shanghai (Nos. 17411962800 and 17ZR1432900), and Science and Technology Commission of Shanghai Municipality (17JC1400200). W.Z. acknowledges funding from the National Natural Science Foundation of China (31501555 and 81772007, and 21734003), the China&#39;s 1000 Young Talents Program, Education Commission of Shanghai Municipality (Young Eastern Professorship Award), and Science and Technology Commission of Shanghai Municipality (17JC1400200 and 16391903900).

The present study was approved by the ethics committee of Zhongshan Hospital Fudan University (B2016-142R) and all participants gave their informed consent.
1. Diagnosis of the Aortic Anomaly by Symptoms and Acquisition of Imaging Data
<ol>
	<li>Identify patients who have symptoms such as chest pain, dysphagia, or a blood pressure difference of the upper limbs in out-patient clinic. Exclude patients who may be intolerant of the operation.</li>
	<li>Perform CT angiography in these patients to...

<ol>
	<li>Tanaka, A., Milner, R., Ota, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Kommerell's+diverticulum+in+the+current+era:+a+comprehensive+review.">Kommerell's diverticulum in the current era: a comprehensive review.</a> General Thoracic and Cardiovascular Surgery. 63 (5), 245-259 (2015).</li><li>Rosu, C., Dorval, J. F., Abraham, C. Z., Cartier, R., Demers, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single-stage+hybrid+repair+of+right+aortic+arch+with+Kommerell's+Diverticulum.">Single-stage hybrid repair of right aortic arch with Kommerell's Diverticulum.</a> The Annals of Thoracic Surgery. 103 (4), e381-e384 (2017).</li><li>Idrees, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hybrid+repair+of+Kommerell+diverticulum.">Hybrid repair of Kommerell diverticulum.</a> The Journal of Thoracic and Cardiovascular Surgery. 147 (3), 973-976 (2014).</li><li>Kankala, R. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fabrication+of+arbitrary+3-D+components+in+cardiac+surgery:+from+macro-,+micro-+to+nanoscale.">Fabrication of arbitrary 3-D components in cardiac surgery: from macro-, micro- to nanoscale.</a> Biofabrication. 9 (3), 032002 (2017).</li><li>Vukicevic, M., Mosadegh, B., Min, J. K., Little, S. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiac+3-D+printing+and+its+future+directions.">Cardiac 3-D printing and its future directions.</a> JACC Cardiovascular Imaging. 10 (2), 171-184 (2017).</li><li>Yoo, S. J., Spray, T., Austin, E. H., Yun, T. J., van Arsdell, G. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hands-on+surgical+training+of+congenital+heart+surgery+using+3-dimensional+print+models.">Hands-on surgical training of congenital heart surgery using 3-dimensional print models.</a> The Journal of Thoracic and Cardiovascular Surgery. 153 (6), 1530-1540 (2017).</li><li>Hermsen, J. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Scan,+print,+practice,+perform:+Development+and+use+of+a+patient-specific+3-dimensionalprinted+model+in+adult+cardiac+surgery.">Scan, print, practice, perform: Development and use of a patient-specific 3-dimensionalprinted model in adult cardiac surgery.</a> The Journal of Thoracic and Cardiovascular Surgery. 153 (1), 132-140 (2017).</li><li>Sun, X., Zhang, H., Zhu, K., Wang, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patient-specific+three-dimensional+printing+for+Kommerell's+diverticulum.">Patient-specific three-dimensional printing for Kommerell's diverticulum.</a> International Journal of Cardiology. 255, 184-187 (2018).</li><li>Ota, T., Okada, K., Takanashi, S., Yamamoto, S., Okita, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+treatment+for+Kommerell's+diverticulum.">Surgical treatment for Kommerell's diverticulum.</a> The Journal of Thoracic and Cardiovascular Surgery. 131 (3), 574-578 (2006).</li><li>Agematsu, K., Ueda, T., Hoshino, S., Nishiya, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rupture+of+Kommerell+diverticulum+after+total+arch+replacement.">Rupture of Kommerell diverticulum after total arch replacement.</a> Interactive Cardiovascular and Thoracic Surgery. 11 (6), 800-802 (2010).</li></ol>

Congenital aortic anomalies comprise a rare spectrum of cardiovascular diseases, which often show complex aortic anomalies. Medical imaging, such as CT and MR, are required to elucidate complex aortic arch anomalies, the abnormal branching pattern, their relationship with the trachea and the esophagus, and other associated pathologies. Both CT and MR angiography can provide 2-D information of aortic vessel locations. With 3-D digital reconstruction of 2-D imaging, the anatomical relationship of the aortic vessels can be ...

Congenital aortic anomalies are extremely rare congenital malformations of the aortic arch system. They can be diagnosed either by imaging analysis or by evaluation of entities like dysphagia or subclavian steal1. In clinical scenarios, it is important to identify the anatomical anomaly in the confined surgical space that has limited visualization during the surgery2,3. Currently, conventional planar two-dimensional (2-D) imaging, such as computed tomography (CT) and magnetic resonance imaging (MRI), are usually presented to surgeons before the surgery. However, it is difficult for surgeons to image the anomaly based on the 2-D imaging. Consequently, they could encounter unpredictable difficulties while trying to separate the complex aortic vessels during surgery. Unpredictable injury to the vessel, the trachea and the esophagus could occur and result in disastrous outcomes.
In the last decade, 3-D imaging modeling has been used in cardiac surgery to help surgeons understand the complex anatomic anomaly4,5,6,7. Three-dimensional (3-D) printing technology can help convert the modeling data into a physical model. Compared with the digital reconstruction, 3-D printed physical models could present a better understanding of the anatomical details and provide an intuitional view of the malformation. For aortic anomaly surgery, the printed intuitional 3-D model is significant because poor understanding of aortic locations could be disastrous to patients. During the surgery, any mistake could lead to unpredictable bleeding and injury. Using the printed models, surgeons can fully understand the spatial relationships of aortic branches. During the surgery, the surgeons can also perform real-time review of the 3-D models to avoid confusion of the complex vascular locations.
Here, we present a protocol to apply 3-D printed models for pre-operative planning and intra-operative guidance while dealing with congenital aortic diseases. Kommerell&#39;s diverticulum, a type of complex congenital aortic anomaly, was selected as a case study. The steps include diagnosis based on computed tomography angiography (CTA) imaging, partitioning regions of interest, building 3-D models, preoperative surgical planning, and intra-operative reviewing of 3-D printed models8. This 3-D printing strategy could substantially reduce the risk of unpredictable tissue injury during the surgery.

<table border="0" cellpadding="0" cellspacing="0" width="911">
	<tbody>
		<tr height="25">
			<td height="25" style="height:25px;width:247px;">3D printer</td>
			<td style="width:231px;">Meditool Enterprise Co., Ltd</td>
			<td style="width:136px;"></td>
			<td style="width:297px;">For 3D printing</td>
		</tr>
		<tr height="25">
			<td height="25" style="height:25px;">Chaos Version 2.0</td>
			<td>Meditool Enterprise Co., Ltd</td>
			<td style="width:136px;"></td>
			<td>For 3D segmentation and reconstruction</td>
		</tr>
	</tbody>
</table>

three-dimensional printing of a complex aortic anomaly

Complex congenital aortic anomalies include diverse types of malformations that may be clinically asymptomatic or present with respiratory or esophageal symptoms. These anomalies may be associated with other congenital heart diseases. It is hard to identify the accurate anatomic vessel location from two-dimensional imaging data, such as computed tomography. As an additive manufacturing method, three-dimensional (3-D) printing can covert the acquired imaging data into 3-D physical models. This protocol describes the procedure for modeling the volumetric DICOM imaging into 3-D data and printing it as an anatomically realistic 3-D model. Using this model, surgeons can identify the vessel location of complex aortic anomalies, which is helpful for pre-operative planning and intra-operative guidance.

Acquisition of CT angiography images, digital modeling and 3-D printing were all done in a hospital. Two hours were spent to get the 3-D model from the CT angiography image ready for the 3-D printing. Using the procedure and 3-D printer here, a patient-specific 3-D physical model can be sent to physicians quickly and the surgical decision can be made in time. The workflow from acquisition of CT angiography data to 3-D printing was shown in Figure 1. From the ...

Watch this Scientific Journal Video about Three-Dimensional Printing of a Complex Aortic Anomaly at JoVE.com

Three-Dimensional Printing of a Complex Aortic Anomaly

Here, we present a protocol to use three dimensional printed models for pre-operative planning and intra-operative reorganization of complicated vascular locations when handling a congenital aortic anomaly.

Complex congenital aortic anomalies include diverse types of malformations that may be clinically asymptomatic or present with respiratory or esophageal ...

This method can help answer key questions in the complex aorta anomaly field about the camera DICOM. The main advantage of this technique is that 3D printed model can reduce the risk of unpredictable tissue injury during the complex aortic surgery. For the segmentation of a region of interest import all of the computed tomography and geography images into the software in a DICOM format and double-click to open the patient case within the case library.Select the DICOM series and click model recon to open the model reconstruction page. Have an engineer and a team of cardiac surgeons review the DICOM formatted raw data to identify key anatomic features and regions of interest. To segment the region, click threshold segmentation, and adjust the threshold range for the vascular mask.Click confirm to generate the vascular mask within the object list and click recon to reconstruct the 3D vascular mask within the 3D viewer. Click threshold segmentation again and adjust the threshold range for the trachea mask. Click marquee segmentation to limit the region of interest to the mediastinum and the lung and click mask edit to erase the connection between the trachea and the lung.Click region grow and click any point of the mask in any one of the 2D viewers to select a seed. Confirm that the region grows as a result and that the trachea mask will show in the object list. Then click recon to reconstruct the 3D trachea in the 3D viewer and save the region of interest as a mask.For 3D reconstruction of the region of interest click export to export the 3D model as a standard triangle language file and position the model on the center of the building platform. Align the tangent of the vessel center line at its extremity so that it is parallel to the Z axis of the platform. Supports will be automatically generated to the overhangs.Click slice and save and set the appropriate 3D printing parameters on a stereolithographic printer. After printing, scan the contours sliced by the software with 405 nanometer ultraviolet light to harden the photosensitive resin at a three meters per second scan rate. Then, before the surgery, have the surgeons use the 3D printed model to make detailed and accurate surgical plans for the patient from which the 3D model was generated.Using images from the coronal plane, the transverse plane, and the sagittal plane, the computed tomography and geography image can be reconstructed into a 3D model as demonstrated. In these images, the anatomic relationship between the aorta and the trachea along the Y axis can be observed. After its development, this technology paved the way for researchers in the field of 3D printing to explore vascular anomaly in aortic surgery.

three-dimensional-printing-of-a-complex-aortic-anomaly

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JoVE Journal

Medicine

6.6K 次观看.  Fudan University. 此方法可以帮助回答有关摄像机 DICOM 的复杂大项异常字段中的关键问题。该技术的主要优点是，3D打印模型可以降低复杂主那手术期间不可预知的组织损伤风险。对于感兴趣的区域的分割，请将所有计算的断层扫描和地理图像以 DICOM 格式导入软件，并双击以打开病例库中的患者病例。选择 DICOM 系列并单击模型重新配置以打开模型重建页面。让一位工程师和一组心脏外?...