Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
Isolation of Crude Thylakoid Membranes from Spinach Leaves
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Solubilization of Thylakoid Membranes for Loading onto Native Gels and Separation of Solubilized Thylakoid Proteins by Native Gel Electrophoresis
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Excision of Thylakoid Complex Bands from Native Green Gels and Collection of Room Temperature Steady-State Fluorescence Spectra
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Time-correlated Single Photon Counting of Green Gel Bands and Data Analysis
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Results: Native Green Gel Electrophoresis and Band Characterization
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Conclusion
副本
This method is useful for answering certain key questions in the field of photosynthesis research such as are putative thylakoid complexes physiologically real complexes with energetically connected components? The main advantage of the green gel
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Here we present a protocol to separate solubilized thylakoid complexes by Native Green Gel electrophoresis. Green gel bands are subsequently characterized by Time Correlated Single Photon Counting (TCSPC) and basic steps for data analysis are provided.