Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Leukoreduction System Chamber (LRSC)
2:34
Magnetic Labeling and Isolation of CD14+ Cells (Monocytes)
4:32
Lysis and In-Gel Digestion
7:54
Results: Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
9:17
Conclusion
Transcript
So this method can help to answer key questions in the macrophage biology field, such as how to characterize the state of activation under various environment at the protein level. The main advantage of this technique is that it provides the oppor
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We present a protocol to obtain proteomic signatures of human macrophages and apply this to determination of the impact of a low oxygen environment on macrophage polarization.
