Preparation of Mouse Endothelial Culture in Permeable Support Inserts
1:55
Set-up of Neural-endothelial Co-culture and Ac4ManNAz Labeling System
3:25
Immunofluorescent Staining of Sialoglycoproteins in Expanded Primary NSPCs and Differentiated Neurons
5:25
Purification of Sialoglycoproteins from Expanded Primary NSPCs and Differentiated Neurons
8:45
Results: Identifying Cell Surface Markers by Metabolic Labeling of Sialoglycan
10:47
Conclusion
副本
Neural stem and progenitor cells can self-renew and differentiate into different neural cell types, supporting nervous system development and offering a resource for regenerative medicine. These cells are heterogeneous, and we need to know their s
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Presented here is a protocol that combines an in vitro neural-endothelial co-culture system and metabolic incorporation of sialoglycan with bioorthogonal functional groups to expand primary neural stem and progenitor cells and label their surface sialoglycoproteins for imaging or mass-spectrometry analysis of cell surface markers.