Cleaning the Coral Skeleton Pieces and Glass Coverslips
2:07
Preparation of Coral Skeleton Grains
2:52
Preparation of Coral Grain‐Coated Dishes or Coverslips
4:40
Cultivation of Hippocampal Dissociated Cells on Coral Skeleton Grain‐Coated Glass Coverslips
7:26
Results: Coral Skeleton Grains and Hippocampal Neural Cells on the Matrix
8:52
Conclusion
Transcript
The significance of this protocol is that it provides a new way to increase the durability and growth of neurons, glia, and stem cells in vitro. It is also applicable for other cells. The main advantage of the technique is the usage of a matrix ma
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Dissociated hippocampal cell culture is a pivotal experimental tool in neuroscience. Neural cell survival and function in culture is enhanced when coralline skeletons are used as matrices, due to their neuroprotective and neuromodulative roles. Hence, neural cells grown on coralline matrix show higher durability, and thereby are more adequate for culturing.
