Using this protocol, researchers can investigate the mechanisms underlying hippocampal network oscillations in acute mouse brain slice preparations. In this preparation, recordings are performed under submerged conditions in slices generating spon
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This protocol describes the preparation of horizontal hippocampal-entorhinal cortex (HEC) slices from mice exhibiting spontaneous sharp-wave ripple activity. Slices are incubated in a simplified interface holding chamber and recordings are performed under submerged conditions with fast-flowing artificial cerebrospinal fluid to promote tissue oxygenation and the spontaneous emergence of network-level activity.