MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
February 23rd, 2021
•MAPS technology has been developed to scrutinize the targetome of a specific regulatory RNA in vivo. The sRNA of interest is tagged with a MS2 aptamer enabling the co-purification of its RNA partners and their identification by RNA sequencing. This modified protocol is particularly suited for Gram-positive bacteria.
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