Hypoxia/Reoxygenation to Simulate Ischemia/Reperfusion Injury In Vitro
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Endpoint Assessment
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Results: Effect of Three Types of Culture Inserts and Different Concentrations of Co-Cultured Endothelial Cells on Assessment of the Release of Lactate Dehydrogenase (LDH)
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Conclusion
副本
Our finding suggests that optimizing the endothelial cell cardiomyocyte co-culture spatial environment is necessary to provide a favorable in vitro model for testing the role of endothelial cells in cardiomyocyte protection against simulated ische
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Spatial distance is a key parameter in assessing hypoxia/reoxygenation injury in a co-culture model of separate endothelial and cardiomyocyte cell layers, suggesting, for the first time, that optimizing the co-culture spatial environment is necessary to provide a favorable in vitro model for testing the role of endothelial cells in cardiomyocyte protection.