Preparation of Perfusion Equipment and Animal Surgery
2:51
Cannulation and Perfusion
5:03
Digestion
6:21
Preparation of the Liver
6:51
Hepatocyte Extraction and Isolation
8:11
Results: A Reliable and Straightforward Method for Isolating High Yield Normal and Steatotic Murine Hepatocytes
9:37
Conclusion
副本
The overall goal of this protocol is to present an optimized method for hepatocyte isolation in mice after partial hepatectomy without the need for density gradient centrifugation. We usually see a large percentage of lipid-laden hepatocytes durin
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Lipid-laden hepatocytes are inherent to liver regeneration but are usually lost upon density-gradient centrifugation. Here, we present an optimized cell isolation protocol that retains steatotic hepatocytes, yielding representative populations of regenerating hepatocytes after partial hepatectomy in mice.
