Methods Article

斯普拉格道利大鼠脂肪组织间充质干细胞的分离和鉴定

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2.9K Views

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10:50 min

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April 7th, 2023

DOI :

10.3791/65172-v

April 7th, 2023

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Aymé Oliva Cárdenas¹^,², Blanca C. Zamora-Rodríguez², Kevin A. Batalla-García², Alejandro Ávalos-Rodríguez³, Alejandra Contreras-Ramos⁴, Clara Ortega-Camarillo²

¹Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, ²Medical Research Unit in Biochemistry, Specialties Hospital, National Medical Center SXXI, Instituto Mexicano de Seguro Social, ³Department of Agricultural and Animal Production, Division of Biological and Health Science, Universidad Autónoma Metropolitana-Xochimilco, ⁴Molecular Biology Research Laboratory, Center for Congenital Malformations, Children Hospital of Mexico Federico Gomez (HIMFG)

副本

摘要

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194

此视频中的章节

0:00

Introduction

1:57

Murine Adipose Tissue Excision

2:36

Murine Adipose‐Derived Mesenchymal Cell Isolation, Maintenance, and Characterization

5:39

Immunofluorescent Detection of Adipose‐Derived Stem Cell Expression Markers

7:09

Flow Cytometric Detection of Adipose‐Derived Stem Cell Expression Markers

9:28

Adipose‐Derived Stem Cell Differentiation to the Adipogenic Lineage

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