Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
3.2K Views
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10:50 min
•
April 7th, 2023
DOI :
April 7th, 2023
•Aymé Oliva Cárdenas1,2, Blanca C. Zamora-Rodríguez2, Kevin A. Batalla-García2, Alejandro Ávalos-Rodríguez3, Alejandra Contreras-Ramos4, Clara Ortega-Camarillo2
1Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, 2Medical Research Unit in Biochemistry, Specialties Hospital, National Medical Center SXXI, Instituto Mexicano de Seguro Social, 3Department of Agricultural and Animal Production, Division of Biological and Health Science, Universidad Autónoma Metropolitana-Xochimilco, 4Molecular Biology Research Laboratory, Center for Congenital Malformations, Children Hospital of Mexico Federico Gomez (HIMFG)
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Chapters in this video
0:00
Introduction
1:57
Murine Adipose Tissue Excision
2:36
Murine Adipose‐Derived Mesenchymal Cell Isolation, Maintenance, and Characterization
5:39
Immunofluorescent Detection of Adipose‐Derived Stem Cell Expression Markers
7:09
Flow Cytometric Detection of Adipose‐Derived Stem Cell Expression Markers
9:28
Adipose‐Derived Stem Cell Differentiation to the Adipogenic Lineage
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