<meta charset="utf8"></head><body>在分期肝切除术动物模型中研究 ALLPS 的肝脏再生

<ol><li>Michalopoulos, G. K., Bhushan, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Liver+regeneration%3A+Biological+and+pathological+mechanisms+and+implications%5BTitle%5D)+AND+%22Nat+Rev+Gastroenterol+Hepatol%22%5BJournal%5D)">Liver regeneration: Biological and pathological mechanisms and implications.</a> Nat Rev Gastroenterol Hepatol. 18 (1), 40-55 (2021).</li>
<li>Chapelle, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Estimation+of+the+future+remnant+liver+function+is+a+better+tool+to+pr+edict+post-hepatectomy+liver+failure+than+platelet-based+liver+scores%5BTitle%5D)+AND+%22Eur+J+Surg+Oncol%22%5BJournal%5D)">Estimation of the future remnant liver function is a better tool to pr edict post-hepatectomy liver failure than platelet-based liver scores.</a> Eur J Surg Oncol. 43 (12), 2277-2284 (2017).</li>
<li>Isfordink, C. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Portal+vein+ligation+versus+portal+vein+embolization+for+induction+of+hypertrophy+of+the+future+liver+remnant%3A+A+systematic+review+and+meta-analysis%5BTitle%5D)+AND+%22Surg+Oncol%22%5BJournal%5D)">Portal vein ligation versus portal vein embolization for induction of hypertrophy of the future liver remnant: A systematic review and meta-analysis.</a> Surg Oncol. 26 (3), 257-267 (2017).</li>
<li>Linecker, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Risk+adjustment+in+alpps+is+associated+with+a+dramatic+decrease+in+ear+ly+mortality+and+morbidity%5BTitle%5D)+AND+%22Ann+Surg%22%5BJournal%5D)">Risk adjustment in alpps is associated with a dramatic decrease in ear ly mortality and morbidity.</a> Ann Surg. 266 (5), 779-786 (2017).</li>
<li>Darnis, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Subtotal+hepatectomy+in+swine+for+studying+small-for-size+syndrome+and+portal+inflow+modulation%3A+Is+it+reliable%5BTitle%5D)+AND+%22HPB+%28Oxford%29%22%5BJournal%5D)">Subtotal hepatectomy in swine for studying small-for-size syndrome and portal inflow modulation: Is it reliable.</a> HPB (Oxford). 17 (10), 881-888 (2015).</li>
<li>Casellas, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Inbred+mouse+strains+and+genetic+stability%3A+A+review%5BTitle%5D)+AND+%22Animal%22%5BJournal%5D)">Inbred mouse strains and genetic stability: A review.</a> Animal. 5 (1), 1-7 (2011).</li>
<li>Zhang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Evolution+of+associating+liver+partition+and+portal+vein+ligation+for+staged+hepatectomy+from+2012+to+2021%3A+A+bibliometric+analysis.+Review%5BTitle%5D)+AND+%22Int+J+Surg%22%5BJournal%5D)">Evolution of associating liver partition and portal vein ligation for staged hepatectomy from 2012 to 2021: A bibliometric analysis. Review.</a> Int J Surg. 103, 106648(2022).</li>
<li>Parola, M., Pinzani, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Liver+fibrosis%3A+Pathophysiology%2C+pathogenetic+targets+and+clinical+iss+ues%5BTitle%5D)+AND+%22Mol+Aspects+Med%22%5BJournal%5D)">Liver fibrosis: Pathophysiology, pathogenetic targets and clinical iss ues.</a> Mol Aspects Med. 65, 37-55 (2019).</li>
<li>Kikuchi, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Impact+of+associating+liver+partition+and+portal+vein+occlusion+for+st+aged+hepatectomy+on+tumor+growth+in+a+mouse+model+of+liver+metastasis%5BTitle%5D)+AND+%22Eur+J+Surg+Oncol%22%5BJournal%5D)">Impact of associating liver partition and portal vein occlusion for st aged hepatectomy on tumor growth in a mouse model of liver metastasis.</a> Eur J Surg Oncol. 44 (1), 130-138 (2018).</li>
<li>Schlegel, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Alpps%3A+From+human+to+mice+highlighting+accelerated+and+novel+mechanism+s+of+liver+regeneration%5BTitle%5D)+AND+%22Ann+Surg%22%5BJournal%5D)">Alpps: From human to mice highlighting accelerated and novel mechanism s of liver regeneration.</a> Ann Surg. 260 (5), 839-846 (2014).</li>
<li>Dili, A., Lebrun, V., Bertrand, C., Leclercq, I. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Associating+liver+partition+and+portal+vein+ligation+for+staged+hepate+ctomy%3A+Establishment+of+an+animal+model+with+insufficient+liver+remnan+t%5BTitle%5D)+AND+%22Lab+Invest%22%5BJournal%5D)">Associating liver partition and portal vein ligation for staged hepate ctomy: Establishment of an animal model with insufficient liver remnan t.</a> Lab Invest. 99 (5), 698-707 (2019).</li>
<li>Schadde, E., Hertl, M., Breitenstein, S., Beck-Schimmer, B., Schläpfer, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rat+model+of+the+associating+liver+partition+and+portal+vein+ligation+for+staged+hepatectomy+%28alpps%29+procedure%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Rat model of the associating liver partition and portal vein ligation for staged hepatectomy (alpps) procedure.</a> J Vis Exp. (126), e55895(2017).</li>
<li>Allaire, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((New+frontiers+in+liver+resection+for+hepatocellular+carcinoma%5BTitle%5D)+AND+%22JHEP+Rep%22%5BJournal%5D)">New frontiers in liver resection for hepatocellular carcinoma.</a> JHEP Rep. 2 (4), 100134(2020).</li>
<li>Memi, F., Ntokou, A., Papangeli, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Crispr%2Fcas9+gene-editing%3A+Research+technologies%2C+clinical+applications+and+ethical+considerations%5BTitle%5D)+AND+%22Semin+Perinatol%22%5BJournal%5D)">Crispr/cas9 gene-editing: Research technologies, clinical applications and ethical considerations.</a> Semin Perinatol. 42 (8), 487-500 (2018).</li>
<li>Saviano, A., Henderson, N. C., Baumert, T. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Single-cell+genomics+and+spatial+transcriptomics%3A+Discovery+of+novel+cell+states+and+cellular+interactions+in+liver+physiology+and+disease+biology%5BTitle%5D)+AND+%22J+Hepatol%22%5BJournal%5D)">Single-cell genomics and spatial transcriptomics: Discovery of novel cell states and cellular interactions in liver physiology and disease biology.</a> J Hepatol. 73 (5), 1219-1230 (2020).</li>
</ol>

<table><tbody><tr><td>5% glucose injection (500 mL)</td><td>Shapuaisi pharma (http://www.zjspas.com/)</td><td>H20003666</td><td>Efficient, cheap,Easy to access</td></tr><tr><td>anaesthesia machine</td><td>RWD (www.rwdls.com)</td><td>R500</td><td>low price and valuable quality. It is suitable for operations with beginners</td></tr><tr><td>C57 BL/6</td><td>The Jackson Lab</td><td>22349-2023</td><td>Stability of strains</td></tr><tr><td>isoflurane</td><td>KCSW (kcsw.szqisoubao.com)</td><td>H19980141</td><td>durable, cost-effective</td></tr><tr><td>meloxicam</td><td>Boehringer-Ingelheim</td><td>H20020217</td><td>Durable and efficient</td></tr><tr><td>microforceps</td><td>maydeal</td><td>60018920</td><td>Durable and efficient</td></tr><tr><td>microinstrument</td><td>CH microsurgical instrument factory</td><td>HC-A804-1</td><td>durable, cost-effective</td></tr><tr><td>sodium lactate ringer</td><td>Shapuaisi pharma (http://www.zjspas.com/)</td><td>H20193277</td><td>Efficient, cheap, easy to access</td></tr><tr><td>suture line</td><td>CH microsurgical instrument factory</td><td>6-0/8-0</td><td>high performance-price ratio</td></tr><tr><td>topical antibodies</td><td>chenxin pharmacology (www.cisen-pharma.com)</td><td>H20020217</td><td>Effectively avoid incision infection</td></tr></tbody></table>

a mouse model of the associating liver partition and portal vein ligation for staged hepatectomy procedure aided by microscopy

Read this Scientific Article Protocol about A Mouse Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy Procedure Aided by Microscopy at JoVE.com

<meta charset="utf8"></head><body>作者聚焦:通过 ALPPS 小鼠模型推进肝脏再生研究

显微镜辅助分期肝切除术的关联肝分区和门静脉结扎的小鼠模型

Hepatectomy is widely regarded as the primary treatment for hepatic malignancies; yet, postoperative liver failure remains a major cause of perioperative ...

a-mouse-model-associating-liver-partition-portal-vein-ligation-for

Research

JoVE Journal

Medicine

A Mouse Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy Procedure Aided by Microscopy

591 次观看.  The First Affiliated Hospital of Nanchang Medical College. ALPPS 可迅速促进肝脏再生。然而，相当小的动物模型和节奏视觉的机制仍然不足。该模型允许研究参与严重肝损伤肝脏再生的分子和细胞途径，与 ARVPS 和 PMX 小鼠模型的 A 一起，商业研究表明，PMX 3 和第二轮的平衡在比较中调节触觉在体质下被杀死，强加手术肝脏再生。单细胞测序。单细胞攻击测序。与单细胞一样，CRISPR 为在正常肝脏和列表?...

视频: 作者聚焦:通过 ALPPS 小鼠模型推进肝脏再生研究

Use of a Hanging-weight System for Liver Ischemia in Mice

Acute liver injury due to ischemia can occur during several clinical procedures e.g. liver transplantation, hepatic tumor resection or trauma repair and can result in liver failure which has a high mortality rate1-2. Therefore murine studies of hepatic ischemia have become an important field of research by providing the opportunity to utilize pharmacological and genetic studies3-9. Specifically, conditional mice with tissue specific deletion of a gene (cre, flox system) provide insights into the role of proteins in particular tissues10-13 . Because of the technical difficulty associated with manually clamping the portal triad in mice, we performed a systematic evaluation using a hanging-weight system for portal triad occlusion which has been previously described3. By using a hanging-weight system we place a suture around the left branch of the portal triad without causing any damage to the hepatic lobes, since also the finest clamps available can cause hepatic tissue damage because of the close location of liver tissue to the vessels. Furthermore, the right branch of the hepatic triad is still perfused thus no intestinal congestion occurs with this technique as blood flow to the right hepatic lobes is preserved. Furthermore, the portal triad is only manipulated once throughout the entire surgical procedure. As a result, procedures like pre-conditioning, with short times of ischemia and reperfusion, can be easily performed. Systematic evaluation of this model by performing different ischemia and reperfusion times revealed a close correlation of hepatic ischemia time with liver damage as measured by alanine (ALT) and aspartate (AST) aminotransferase serum levels3,9. Taken together, these studies confirm highly reproducible liver injury when using the hanging-weight system for hepatic ischemia and intermittent reperfusion. Thus, this technique might be useful for other investigators interested in liver ischemia studies in mice. Therefore the video clip provides a detailed step-by-step description of this technique.

We established a novel murine model of a hanging weight system for portal triad occlusion. This technique may be useful for future investigations of ischemia in murine hepatic models.

使用吊重系统在小鼠肝缺血

Acute liver injury due to ischemia can occur during several clinical procedures e.g. liver transplantation, hepatic tumor resection or trauma repair and ...

科研

医学

Cecal Ligation Puncture Procedure

Human sepsis is characterized by a set of systemic reactions in response to intensive and massive infection that failed to be locally contained by the host. Currently, sepsis ranks among the top ten causes of mortality in the USA intensive care units 1. During sepsis there are two established haemodynamic phases that may overlap. The initial phase (hyperdynamic) is defined as a massive production of proinflammatory cytokines and reactive oxygen species by macrophages and neutrophils that affects vascular permeability (leading to hypotension), cardiac function and induces metabolic changes culminating in tissue necrosis and organ failure. Consequently, the most common cause of mortality is acute kidney injury. The second phase (hypodynamic) is an anti-inflammatory process involving altered monocyte antigen presentation, decreased lymphocyte proliferation and function and increased apoptosis. This state known as immunosuppression or immune depression sharply increases the risk of nocosomial infections and ultimately, death. The mechanisms of these pathophysiological processes are not well characterized. Because both phases of sepsis may cause irreversible and irreparable damage, it is essential to determine the immunological and physiological status of the patient. This is the main reason why many therapeutic drugs have failed. The same drug given at different stages of sepsis may be therapeutic or otherwise harmful or have no effect 2,3. To understand sepsis at various levels it is crucial to have a suitable and comprehensive animal model that reproduces the clinical course of the disease. It is important to characterize the pathophysiological mechanisms occurring during sepsis and control the model conditions for testing potential therapeutic agents. 
To study the etiology of human sepsis researchers have developed different animal models. The most widely used clinical model is cecal ligation and puncture (CLP). The CLP model consists of the perforation of the cecum allowing the release of fecal material into the peritoneal cavity to generate an exacerbated immune response induced by polymicrobial infection. This model fulfills the human condition that is clinically relevant. As in humans, mice that undergo CLP with fluid resuscitation show the first (early) hyperdynamic phase that in time progresses to the second (late) hypodynamic phase. In addition, the cytokine profile is similar to that seen in human sepsis where there is increased lymphocyte apoptosis (reviewed in 4,5). Due to the multiple and overlapping mechanisms involved in sepsis, researchers need a suitable sepsis model of controlled severity in order to obtain consistent and reproducible results.

The mouse model of cecal ligation and puncture as a valuable tool for the study of human sepsis.

盲肠结扎穿刺过程

Human sepsis is characterized by a set of systemic reactions in response to intensive and massive infection that failed to be locally contained by the ...

Partial Lobular Hepatectomy: A Surgical Model for Morphologic Liver Regeneration

Morphological organ regeneration following acute tissue loss is common among lower vertebrates, but is rarely observed in mammalian postnatal life. Adult liver regeneration after 70% partial hepatectomy results in hepatocyte hypertrophy with some replication in remaining lobes with restoration of metabolic activity, but with permanent loss of the injured lobe&#39;s morphology and architecture. Here, we detail a new surgical method in the neonate that leaves a physiologic environment conducive to regeneration. This model involves amputation of the left lobe apex and a subsequent conservative management regimen, and lacks the necessity for ligation of major liver vessels or chemical injury, leaving a physiologic environment where regeneration may occur. We extend this protocol to amputations on juvenile (P7-14) mice, during which the injured liver transitions from organ regeneration to compensatory growth by hypertrophy. The presented, brief 30 min protocol provides a framework to study the mechanisms of regeneration, its age-associated decline in mammals, and the characterization of putative hepatic stem or progenitors.

Here, we present a new method for partial resection of the left hepatic lobe in neonatal (day 0) mice. This new protocol is suitable for studying acute liver injury and injury response in the neonatal setting.

部分小叶切除术: 形态学肝再生的外科模型

Morphological organ regeneration following acute tissue loss is common among lower vertebrates, but is rarely observed in mammalian postnatal life. Adult ...

发育生物学

<meta charset="utf8"></head><body>用于肝再生研究的患者来源肝癌细胞类器官

A Hepatocellular Cancer Patient&#45;Derived Organoid Xenograft Model to Investigate Impact of Liver Regeneration on Tumor Growth

Recurrence poses a notable challenge after hepatocellular carcinoma (HCC) treatment, impacting more than 70% of patients who undergo surgical resection. Recurrence stems from undetected micro-metastasis or de novo cancer, potentially triggered by postsurgical liver regeneration. Prior research employed HCC cell lines in orthotopic models to study the impact of liver regeneration, but their limited validity prompted the need for a more representative model. Here, we introduce a novel approach utilizing patient-derived HCC organoids to investigate the influence of liver regeneration on HCC.
Patient tumor tissues are processed to create tumor organoids, embedded in a three-dimensional basement membrane matrix, and cultured in a liver-specific medium. One million organoids are injected into the right superior lobe (RSL) of immunodeficient mice, confirming macroscopic tumor growth through sonography. The intervention group undergoes resection of the left lateral lobe (LLL) (30% of total liver volume) or additionally, the middle lobe (ML) (65% of total liver volume) to induce liver regeneration within the tumor site. The control group experiences re-laparotomy without liver tissue resection. After 2 weeks, both groups undergo tumor and normal tissue explantation.
In conclusion, this patient-derived HCC organoid model offers a robust platform to investigate the impact of liver regeneration post-cancer resection. Its multi-cellular composition, genetic diversity, and prolonged culture capabilities make it an invaluable tool for studying HCC recurrence mechanisms and potential interventions.

<meta charset="utf8"></head><body>肝细胞癌患者来源的类器官异种移植模型，用于研究肝脏再生对肿瘤生长的影响

Organoids generated from patient tumors are orthotopically injected into the mouse liver. The resection of non-tumorous liver tissue leads to a regenerative environment in the liver tissue where the tumor is located.

肝细胞癌患者来源的类器官异种移植模型，用于研究肝脏再生对肿瘤生长的影响

Recurrence poses a notable challenge after hepatocellular carcinoma (HCC) treatment, impacting more than 70% of patients who undergo surgical resection. ...

癌症研究

Visualization of Vascular and Parenchymal Regeneration after 70% Partial Hepatectomy in Normal Mice

A modified silicone injection procedure was used for visualization of the hepatic vascular tree. This procedure consisted of in-vivo injection of the silicone compound, via a 26 G catheter, into the portal or hepatic vein. After silicone injection, organs were explanted and prepared for ex-vivo micro-CT (&#181;CT) scanning. The silicone injection procedure is technically challenging. Achieving a successful outcome requires extensive microsurgical experience from the surgeon. One of the challenges of this procedure involves determining the adequate perfusion rate for the silicone compound. The perfusion rate for the silicone compound needs to be defined based on the hemodynamic of the vascular system of interest. Inappropriate perfusion rate can lead to an incomplete perfusion, artificial dilation and rupturing of vascular trees.
The 3D reconstruction of the vascular system was based on CT scans and was achieved using preclinical software such as HepaVision. The quality of the reconstructed vascular tree was directly related to the quality of silicone perfusion. Subsequently computed vascular parameters indicative of vascular growth, such as total vascular volume, were calculated based on the vascular reconstructions. Contrasting the vascular tree with silicone allowed for subsequent histological work-up of the specimen after &#181;CT scanning. The specimen can be subjected to serial sectioning, histological analysis and whole slide scanning, and thereafter to 3D reconstruction of the vascular trees based on histological images. This is the prerequisite for the detection of molecular events and their distribution with respect to the vascular tree. This modified silicone injection procedure can also be used to visualize and reconstruct the vascular systems of other organs. This technique has the potential to be extensively applied to studies concerning vascular anatomy and growth in various animal and disease models.

Tools used for visualizing vascular regeneration require methods for contrasting the vascular trees. This film demonstrated a delicate injection technique used to achieve optimal contrasting of the vascular trees and illustrate the potential benefits resulting from a detailed analysis of the resulting specimen using &#181;CT and histological serial sections.

之后，正常小鼠70％部分肝切除血管和实质再生的可视化

A modified silicone injection procedure was used for visualization of the hepatic vascular tree. This procedure consisted of in-vivo injection of the ...

Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) Procedure

Recent clinical data support an aggressive surgical approach to both primary and metastatic liver tumors. For some indications, like colorectal liver metastases, the amount of liver tissue left behind after liver resection has become the main limiting factor of resectability of large or multiple liver tumors. A minimal amount of functional tissue is required to avoid the severe complication of post-hepatectomy liver failure, which has high morbidity and mortality. Inducing liver growth of the prospective remnant prior to resection has become more established in liver surgery, either in the form of portal vein embolization by interventional radiologists or in the form of portal vein ligation several weeks prior to resection. Recently, it was shown that liver regeneration is more extensive and rapid, when the parenchymal transection is added to portal vein ligation in a first stage and then, after only one week of waiting, resection performed in a second stage (Associating Liver Partition and Portal vein ligation for Staged hepatectomy = ALPPS). ALPPS has rapidly become popular across the world, but has been criticized for its high perioperative mortality. The mechanism of accelerated and extensive growth induced by this procedure has not been well understood. Animal models have been developed to explore both the physiological and molecular mechanisms of accelerated liver regeneration in ALPPS. This protocol presents a rat model that allows mechanistic exploration of accelerated regeneration.

Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy ALPPS Procedure

Inducing rapid liver hypertrophy using Associating Liver Partition and Portal vein ligation for a Staged hepatectomy (ALPPS) has been proposed for resection of borderline resectable liver tumors. This model may elucidate mechanisms involved in rapid hypertrophy and allows testing of drugs that promote or block the acceleration of regeneration.

肝分型与门静脉结扎大鼠分期肝切除 (ALPPS) 手术模型的建立

Recent clinical data support an aggressive surgical approach to both primary and metastatic liver tumors. For some indications, like colorectal liver ...

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia

Biliary atresia (BA) is a fatal disease involving obstructive jaundice, and it is the most common indication for liver transplantation in children. Due to the complex etiology and unknown pathogenesis, there are still no effective drug treatments. At present, the classic BA mouse model induced by rhesus rotavirus (RRV) is the most commonly used model for studying the pathogenesis of BA. This model is characterized by growth retardation, jaundice of the skin and mucosa, clay stools, and dark yellow urine. The histopathology shows severe liver inflammation and obstruction of the intrahepatic and extrahepatic bile ducts, which are similar to the symptoms of human BA. However, the livers of end-stage mice in this model lack fibrosis and cannot fully simulate the characteristics of liver fibrosis in clinical BA. The presented study developed a novel BA mouse model of chronic liver fibrosis by injecting 5-10 &#181;g of anti-Ly6G antibody four times, with gaps of 2 days after each injection. The results showed that some of the mice successfully formed chronic BA with typical fibrosis after the time lapse, meaning these mice represent a suitable animal model for the virus-induced liver fibrosis mechanistic study of BA and a platform for developing future BA treatments.

We established a mouse model of chronic liver fibrosis, which provides a suitable animal model for the virus-induced liver fibrosis mechanistic study of biliary atresia (BA) and a platform for future BA treatments.

慢性肝纤维化小鼠模型用于研究胆道闭锁

Biliary atresia (BA) is a fatal disease involving obstructive jaundice, and it is the most common indication for liver transplantation in children. Due to ...

免疫与感染

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

Non-alcoholic steatohepatitis (NASH) is the most common chronic liver disease in the United States, affecting more than 70 million Americans. NASH can progress to fibrosis and eventually to cirrhosis, a significant risk factor for hepatocellular carcinoma. The extracellular matrix (ECM) provides structural support and maintains liver homeostasis via matricellular signals. Liver fibrosis results from an imbalance in the dynamic ECM remodeling process and is characterized by excessive accumulation of structural elements and associated changes in glycosaminoglycans. The typical fibrosis pattern of NASH is called &#34;chicken wire,&#34; which usually consists of zone 3 perisinusoidal/pericellular fibrosis, based on features observed by Masson&#39;s trichrome stain and Picrosirius Red stains. However, these traditional thin two-dimensional (2D) tissue slide-based imaging techniques cannot demonstrate the detailed three-dimensional (3D) ECM structural changes, limiting the understanding of the dynamic ECM remodeling in liver fibrosis.
The current work optimized a fast and efficient protocol to image the native ECM structure in the liver via decellularization to address the above challenges. Mice were fed either with chow or fast-food diet for 14 weeks. Decellularization was performed after in situ portal vein perfusion, and the two-photon microscopy techniques were applied to image and analyze changes in the native ECM. The 3D images of the normal and NASH livers were reconstituted and analyzed. Performing in situ perfusion decellularization and analyzing the scaffold by two-photon microscopy provided a practical and reliable platform to visualize the dynamic ECM remodeling in the liver.

The present protocol optimizes the liver in situ perfusion/decellularization and two-photon microscopy methods to establish a reliable platform to visualize the dynamics of extracellular matrix (ECM) remodeling during non-alcoholic steatohepatitis (NASH).

非酒精性脂肪性肝炎小鼠模型中肝细胞外基质的 3D 成像

Non-alcoholic steatohepatitis (NASH) is the most common chronic liver disease in the United States, affecting more than 70 million Americans. NASH can ...

显微镜辅助分期肝切除术的关联肝分区和门静脉结扎的小鼠模型

副本

摘要

探索更多视频

使用吊重系统在小鼠肝缺血

盲肠结扎穿刺过程

部分小叶切除术: 形态学肝再生的外科模型

肝细胞癌患者来源的类器官异种移植模型，用于研究肝脏再生对肿瘤生长的影响

肝细胞癌患者来源的类器官异种移植模型，用于研究肝脏再生对肿瘤生长的影响

肝细胞癌患者来源的类器官异种移植模型，用于研究肝脏再生对肿瘤生长的影响

之后，正常小鼠70％部分肝切除血管和实质再生的可视化

肝分型与门静脉结扎大鼠分期肝切除 (ALPPS) 手术模型的建立

慢性肝纤维化小鼠模型用于研究胆道闭锁

非酒精性脂肪性肝炎小鼠模型中肝细胞外基质的 3D 成像