<meta charset="utf8"></head><body>ICSI-ET 小鼠模型研究对葡萄糖代谢的长期影响

<ol>
	<li>Palermo, G., Joris, H., Devroey, P., Van Steirteghem, A. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pregnancies+after+intracytoplasmic+injection+of+single+spermatozoon+into+an+oocyte.">Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte.</a> Lancet. 340 (8810), 17-18 (1992).</li><li>Flurkey, K. M., Currer, J., Harrison, D. E., Fox, J. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vol.+III+Mouse+models+in+aging+research.+Ch.+20.">Vol. III Mouse models in aging research. Ch. 20.</a> The mouse in biomedical research (second edition). , 637-672 (2007).</li><li>Chen, M., Heilbronn, L. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+health+outcomes+of+human+offspring+conceived+by+assisted+reproductive+technologies+(art).">The health outcomes of human offspring conceived by assisted reproductive technologies (art).</a> J Dev Orig Health Dis. 8 (4), 388-402 (2017).</li><li>Heber, M. F., Ptak, G. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effects+of+assisted+reproduction+technologies+on+metabolic+health+and+diseasedagger.">The effects of assisted reproduction technologies on metabolic health and diseasedagger.</a> Biol Reprod. 104 (4), 734-744 (2021).</li><li>Ma, R. C. W., Ng, N. Y. H., Cheung, L. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assisted+reproduction+technology+and+long-term+cardiometabolic+health+in+the+offspring.">Assisted reproduction technology and long-term cardiometabolic health in the offspring.</a> PLoS Med. 18 (9), e1003724 (2021).</li><li>De Waal, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+cumulative+effect+of+assisted+reproduction+procedures+on+placental+development+and+epigenetic+perturbations+in+a+mouse+model.">The cumulative effect of assisted reproduction procedures on placental development and epigenetic perturbations in a mouse model.</a> Hum Mol Genet. 24 (24), 6975-6985 (2015).</li><li>Carlin, J., George, R., Reyes, T. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methyl+donor+supplementation+blocks+the+adverse+effects+of+maternal+high+fat+diet+on+offspring+physiology.">Methyl donor supplementation blocks the adverse effects of maternal high fat diet on offspring physiology.</a> PLoS One. 8 (5), e63549 (2013).</li><li>Hiraoka, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Piezo-icsi+for+human+oocytes.">Piezo-icsi for human oocytes.</a> J Vis Exp. (170), e60224 (2021).</li><li>Sullivan-Pyke, C. S., Senapati, S., Mainigi, M. A., Barnhart, K. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+fertilization+and+adverse+obstetric+and+perinatal+outcomes.">In vitro fertilization and adverse obstetric and perinatal outcomes.</a> Semin Perinatol. 41 (6), 345-353 (2017).</li><li>Luke, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+risk+of+birth+defects+with+conception+by+art.">The risk of birth defects with conception by art.</a> Hum Reprod. 36 (1), 116-129 (2021).</li><li>Norrman, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiovascular+disease,+obesity,+and+type+2+diabetes+in+children+born+after+assisted+reproductive+technology:+A+population-based+cohort+study.">Cardiovascular disease, obesity, and type 2 diabetes in children born after assisted reproductive technology: A population-based cohort study.</a> PLoS Med. 18 (9), e1003723 (2021).</li><li>Goldsmith, S., Mcintyre, S., Badawi, N., Hansen, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cerebral+palsy+after+assisted+reproductive+technology:+A+cohort+study.">Cerebral palsy after assisted reproductive technology: A cohort study.</a> Dev Med Child Neurol. 60 (1), 73-80 (2018).</li><li>Hansen, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intellectual+disability+in+children+conceived+using+assisted+reproductive+technology.">Intellectual disability in children conceived using assisted reproductive technology.</a> Pediatrics. 142 (6), e20181269 (2018).</li><li>Chang, R. C., Wang, H., Bedi, Y., Golding, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preconception+paternal+alcohol+exposure+exerts+sex-specific+effects+on+offspring+growth+and+long-term+metabolic+programming.">Preconception paternal alcohol exposure exerts sex-specific effects on offspring growth and long-term metabolic programming.</a> Epigenetics Chromatin. 12 (1), 9 (2019).</li><li>Grasemann, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Parental+diabetes:+The+akita+mouse+as+a+model+of+the+effects+of+maternal+and+paternal+hyperglycemia+in+wildtype+offspring.">Parental diabetes: The akita mouse as a model of the effects of maternal and paternal hyperglycemia in wildtype offspring.</a> PLoS One. 7 (11), e50210 (2012).</li></ol>

<table><tbody><tr><td>1.25% avertin (2,2,2-tribromoethanol)</td><td>Nanjing Aibei</td><td>M2960</td><td>for anesthetization</td></tr><tr><td>Bacteriological Petri Dishes 35 x 10 mm style w/tight lid, crystal-grade virgin polystyrene, sterile</td><td>BD</td><td>353001</td><td></td></tr><tr><td>Bacteriological Petri Dishes 50 x 9 mm style w/tight lid, crystal-grade virgin polystyrene, sterile</td><td>BD</td><td>351006</td><td></td></tr><tr><td>Biosafety Cabinet</td><td>ESCO</td><td>class &lt;img alt=&quot;Equation 1&quot; src=&quot;/files/ftp_upload/66212/66212eq01.jpg&quot; style=&quot;margin: auto;&quot;/&gt; BSC</td><td>Aseptic operations, making culture dishes, aliquoting reagents, etc.</td></tr><tr><td>CO2 Incubator</td><td>Thermo</td><td>8000DH</td><td>Embryo culture</td></tr><tr><td>Dissection Microscope</td><td>Olympus</td><td>SZX16</td><td>Use in mouse embryo transfer</td></tr><tr><td>Fluorinert Fc-770</td><td>SIGMA</td><td>F3556</td><td>Fluorinert FC-770 is a thermally stable fully fluorinated liquid with high dielectric strength and resistivity, used as operating fluid</td></tr><tr><td>handheld glucometer</td><td>Roche</td><td>AccuChek performa</td><td>Blood glucose measurement</td></tr><tr><td>HTF</td><td>Merck</td><td>MR-070-D</td><td>The EmbryoMax Human Tubal Fluid (HTF) (1x), liquid designed for use with Mouse IVF is available in a 50 mL format and has been optimized and validated for Embryo Culture.</td></tr><tr><td>Hydraulic Microinjector</td><td>Eppendorf</td><td>CellTram 4r Oil, 5196000030</td><td>For sperm injection</td></tr><tr><td>Inverted Microscope</td><td>Nikon</td><td>TI2-U</td><td>Micromanipulation observation host</td></tr><tr><td>KSOM</td><td>Merck</td><td>MR-020P-D</td><td>(1x), Powder, w/o Phenol Red, 5 x 10 mL</td></tr><tr><td>M2</td><td>Merck</td><td>MR-015-D</td><td>EmbryoMax M2 Medium (1x), Liquid, with Phenol Red</td></tr><tr><td>Micromanipulator</td><td>NARISHIGE</td><td>NTX-N4</td><td>Micromanipulation arm</td></tr><tr><td>mineral oil&amp;nbsp;</td><td>SIGMA</td><td>M8410</td><td>Mineral oil is suitable for use as a cover layer to control evaporation and cross-contamination in various molecular biology applications.</td></tr><tr><td>Needle Cutter</td><td>Nanjing Aibei</td><td>Sutter MF-800</td><td>Use for fabricating micromanipulation needles</td></tr><tr><td>Needle Puller</td><td>Nanjing Aibei</td><td>Sutter model p-100</td><td>Use for making micromanipulation needles</td></tr><tr><td>Piezo Drill Trip Mouce ICSI</td><td>Eppendorf</td><td>5195000.087</td><td>Application of ICSI injection needle.</td></tr><tr><td>Piezoelectric Micromanipulator (Membrane Breaker)</td><td>Eppendorf</td><td>Eppendorf PiezoXpert</td><td>Use of micro-pulses to break the zona pellucida and oolemma of the oocyte</td></tr><tr><td>Pneumatic Microinjector</td><td>Eppendorf</td><td>CellTram 4r Air, 5196000013</td><td>For fixing the oocyte</td></tr><tr><td>Ready-to-use Human Chorionic Gonadotropin (Hcg)</td><td>Nanjing Aibei</td><td>M2520</td><td>Sterilization reagent, intraperitoneal injection. 50 IU/mL</td></tr><tr><td>Ready-to-use Pregnant Mare&#039;s Serum Gonadotropin (PMSG)</td><td>Nanjing Aibei</td><td>M2620</td><td>Sterilization reagent, intraperitoneal injection. 50 IU/mL</td></tr><tr><td>Stereomicroscope</td><td>Olympus</td><td>SZX7</td><td>Oocyte retrieval and observation of embryo development</td></tr></tbody></table>

monitoring blood glucose in mouse offspring after intracytoplasmic sperm injection

Read this Scientific Article Protocol about Monitoring Blood Glucose in Mouse Offspring after Intracytoplasmic sperm injection - Embryo Transfer at JoVE.com

<meta charset="utf8"></head><body>作者聚焦:探索卵胞浆内单精子注射对后代的长期健康影响

监测胞浆内单精子注射后小鼠后代的血糖

Human lifespan is considerably long, while mouse models can simulate the entire human lifespan in a relatively short period, with one year of mouse life ...

monitoring-blood-glucose-mouse-offspring-after-intracytoplasmic-sperm

Research

JoVE Journal

Developmental Biology

Monitoring Blood Glucose in Mouse Offspring After Intracytoplasmic Sperm Injection

448 次观看.  Shanghai-MOST Key Laboratory of Health and Disease Genomics. 我们正在研究 ICSI 中使用的技术是否会导致后代代谢紊乱。这真的很重要，因为它确保了辅助生殖技术的安全和发展。我们的研究为未来的研究奠定了坚实的基础，使研究人员能够探索精子如何随着时间的推移对胚胎生长和后代健康产生影响。我们的团队将专注于 ICSI 后代的心血管健康和免疫系统结果，旨在全面了解他们的长期健康状况。

视频: 作者聚焦:探索卵胞浆内单精子注射对后代的长期健康影响

<meta charset="utf8"></head><body>小鼠圆形精子细胞注射程序研究胚胎发育

Mouse Round Spermatid Injection

Round spermatids, characterized by their haploid genetic content, represent the precursor cells to mature spermatozoa. Through the innovative technique of round spermatid injection (ROSI), oocytes can be successfully fertilized and developed into viable fetuses. In a groundbreaking milestone achieved in 1995, the first mouse fetus was born through ROSI technology. ROSI has since emerged as a pivotal tool for unraveling the intricate mechanisms governing embryonic development and holds significant potential in various applications, including the acceleration of mouse generation and the production of genetically modified mice. In 1996, a milestone was reached when the first human fetus was born through ROSI technology. However, the clinical applications of this method have shown a fluctuating pattern of success and failure. To date, ROSI technology has not found widespread application in clinical practice, primarily due to its low birth efficiency and insufficient validation of fetal safety. This article provides a comprehensive account of the precise methods of performing ROSI in mice, aiming to shed new light on basic research and its potential clinical applications.

<meta charset="utf8"></head><body>小鼠圆形精子细胞注射

Here, we introduce a method for performing round spermatid injection (ROSI) in mice, a technique with promising clinical applications and utility for investigating the mechanisms underlying embryonic development.

小鼠圆形精子细胞注射

Round spermatids, characterized by their haploid genetic content, represent the precursor cells to mature spermatozoa. Through the innovative technique of ...

科研

发育生物学

An In Ovo Model for Testing Insulin-mimetic Compounds

Elevated blood glucose levels in type 2 diabetes mellitus (T2DM), a complex and multifactorial metabolic disease, are caused by insulin resistance and &#946;-cell failure. Various strategies, including the injection of insulin or the usage of insulin-sensitizing drugs, were pursued to treat T2DM or at least reduce the symptoms. In addition, the application of herbal compounds has attracted increasing attention. Thus, it is necessary to find efficient test systems to identify and characterize insulin-mimetic compounds. Here we developed a modified chick embryo model, which enables testing of synthetic compounds and herbal extracts with insulin-mimetic properties. Using a fluorescence microscopy-based primary screen, which quantifies the translocation of Glucose transporter 4 (Glut4) to the plasma membrane, we were able to identify compounds, mainly herbal extracts, which lead to an increase of intracellular glucose concentrations in adipocytes. However, the efficacy of these substances requires further verification in a living organism. Thus, we used an in-ovo approach to identify their blood glucose-reducing properties. The approval by an ethics committee is not needed since the use of chicken embryos during the first two-thirds of embryonic development is not considered an animal experiment. Here, the application of this model is described in detail.

An In Ovo Model for Testing Insulin-mimetic Compounds

In this study, we describe an in-ovo system as a promising tool to test insulin-mimetic compounds in a living organism. The main advantages include adequate throughput rates and acceptable costs, which enable the identification of phytochemicals with insulin-mimetic properties.

用于测试胰岛素模拟化合物的蛋模型

Elevated blood glucose levels in type 2 diabetes mellitus (T2DM), a complex and multifactorial metabolic disease, are caused by insulin resistance and ...

神经科学

Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain

Insulin regulates systematic metabolism in the hypothalamus and the peripheral insulin response. An inflammatory reaction in peripheral adipose tissues contributes to type 2 diabetes mellitus (T2DM) development and appetite regulation in the hypothalamus. Chemokine CCL5 and C-C chemokine receptor type 5 (CCR5) levels have been suggested to mediate arteriosclerosis and glucose intolerance in type 2 diabetes mellitus (T2DM). In addition, CCL5 plays a neuroendocrine role in the hypothalamus by regulating food intake and body temperature, thus, prompting us to investigate its function in hypothalamic insulin signaling and the regulation of peripheral glucose metabolism.
The micro-osmotic pump brain infusion system is a quick and precise way to manipulate CCL5 function and study its effect in the brain. It also provides a convenient alternative approach to generating a transgenic knockout animal. In this system, CCL5 signaling was blocked by intracerebroventricular (ICV) infusion of its antagonist, MetCCL5, using a micro-osmotic pump. The peripheral glucose metabolism and insulin responsiveness was detected by the Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT). Insulin signaling activity was then analyzed by protein blot from tissue samples derived from the animals.
After 7-14 days of MetCCL5 infusion, the glucose metabolism and insulin responsiveness was impaired in mice, as seen in the results of the OGTT and ITT. The IRS-1 serine302 phosphorylation was increased and the Akt activity was reduced in mice hypothalamic neurons following CCL5 inhibition. Altogether, our data suggest that blocking CCL5 in the mouse brain increases the phosphorylation of IRS-1 S302 and interrupts hypothalamic insulin signaling, leading to a decrease in insulin function in peripheral tissues as well as the impairment of glucose metabolism.

This protocol studies the role of chemokine (C-C motif) ligand 5 (CCL5) in the hypothalamus by delivering an antagonist, MetCCL5, into the mouse brain using a micro-osmotic pump brain infusion system. This transient inhibition of CCL5 activity interrupted hypothalamic insulin signaling, leading to glucose intolerance and peripheral systemic insulin sensitivity.

持续药物输注系统对小鼠脑内下丘脑胰岛素信号的研究

Insulin regulates systematic metabolism in the hypothalamus and the peripheral insulin response. An inflammatory reaction in peripheral adipose tissues ...

医学

Acclimation Prior to an Intraperitoneal Insulin Tolerance Test to Mitigate Stress-Induced Hyperglycemia in Conscious Mice

The insulin tolerance test is commonly used in metabolic studies to assess whole body insulin sensitivity in rodents. It is a relatively simple test that involves measurement of blood glucose levels over time following a single intraperitoneal injection of insulin. Given that it is performed in the conscious state and blood is often collected via a tail snip, it has the potential to elicit a stress response from animals due to anxiety associated with handling and blood collection. As such, a stress-induced rise in blood glucose can occur, making it difficult to detect and interpret the primary endpoint measure, namely an insulin-mediated reduction in blood glucose. This has been seen in many mouse strains, and is quite common in diabetic db/db mice, where glucose levels can increase, rather than decrease, after insulin administration. Here, we describe a method of acclimating mice to handling, injections and blood sampling prior to performing the insulin tolerance test. We find that this lowers stress-induced hyperglycemia and results in data that more accurately reflects whole body insulin sensitivity.

Stress-induced elevations in glucose levels can confound interpretation of data derived from a conscious intraperitoneal insulin tolerance test in mice. In this article, we describe a method to acclimate the mice to handling, injections and blood sampling prior to performing the insulin tolerance test in order to limit stress-induced hyperglycemia.

The insulin tolerance test is commonly used in metabolic studies to assess whole body insulin sensitivity in rodents. It is a relatively simple test that ...

Generating a Reproducible Model of Mid-Gestational Maternal Immune Activation using Poly(I:C) to Study Susceptibility and Resilience in Offspring

Maternal immune activation (MIA) during pregnancy is consistently linked to increased risk of neurodevelopmental and neuropsychiatric disorders in offspring. Animal models of MIA are used to test causality, investigate mechanisms, and develop diagnostics and treatments for these disorders. Despite their widespread use, many MIA models suffer from a lack of reproducibility and almost all ignore two important aspects of this risk factor: (i) many offspring are resilient to MIA, and (ii) susceptible offspring can exhibit distinct combinations of phenotypes. To increase reproducibility and model both susceptibility and resilience to MIA, the baseline immunoreactivity (BIR) of female mice before pregnancy is used to predict which pregnancies will result in either resilient offspring or offspring with defined behavioral and molecular abnormalities after exposure to MIA. Here, a detailed method of inducing MIA via intraperitoneal (i.p.) injection of the double stranded RNA (dsRNA) viral mimic poly(I:C) at 12.5 days of gestation is provided. This method induces an acute inflammatory response in the dam, which results in perturbations in brain development in mice that map onto similarly impacted domains in human psychiatric and neurodevelopmental disorders (NDDs).

Generating a Reproducible Model of Mid-Gestational Maternal Immune Activation using PolyI:C to Study Susceptibility and Resilience in Offspring

Maternal infection is a risk factor for neurodevelopmental disorders. Mouse models of maternal immune activation (MIA) may elucidate infection's impact on brain development and function. Here, general guidelines and a procedure are provided to produce reliably resilient and susceptible offspring exposed to MIA.

使用聚（I:C）生成妊娠中期母体免疫激活的可重复模型，以研究后代的易感性和恢复力

Maternal immune activation (MIA) during pregnancy is consistently linked to increased risk of neurodevelopmental and neuropsychiatric disorders in ...

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model

Polycystic ovary syndrome (PCOS) is a common disease that results in disorders of glucose metabolism, such as insulin resistance and glucose intolerance. Dysregulated glucose metabolism is an important manifestation of the disease and is the key to its pathogenesis. Therefore, studies involving evaluation of glucose metabolism in PCOS are of utmost importance. Very few studies have quantified hepatic glucose production directly in PCOS models using non-radioactive glucose tracers. In this study, we discuss step-by-step instructions for the quantification of the rate of hepatic glucose production in a PCOS mouse model by measuring M+2 enrichment of [6,6-2H2]glucose, a stable isotopic glucose tracer, via gas chromatography - mass spectrometry (GCMS). This procedure involves creation of stable isotopic glucose tracer solution, use of tail vein catheter placement and infusion of the glucose tracer in both fasting and glucose-rich states in the same mouse in tandem. The enrichment of [6,6-2H2]glucose is measured using pentaacetate derivative in GCMS. This technique can be applied to a wide variety of studies involving direct measurement of the rate of hepatic glucose production.

This study describes the direct measurement of hepatic glucose production in a polycystic ovary syndrome mouse model by using a stable isotopic glucose tracer via tail vein in both fasting and glucose-rich states in tandem.

多囊卵巢综合征小鼠模型中肝葡萄糖产生的评价

Polycystic ovary syndrome (PCOS) is a common disease that results in disorders of glucose metabolism, such as insulin resistance and glucose intolerance. ...

Detecting Establishment of Shared Blood Supply in Parabiotic Mice by Caudal Vein Glucose Injection

Parabiosis is an experimental method for surgically combining two parallel animals along the longitudinal axis of the body. We present a protocol for detecting the successful establishment of blood chimerism in parabionts by a caudal vein injection of glucose. Parabiotic mice were constructed. Glucose was injected into the donor mouse through the tail vein, and the fluctuation of blood glucose level was measured in both mice using a blood glucometer at different time points. Our results showed that after glucose injection, the blood glucose level in donor mice increased sharply after 1 min and decreased slowly thereafter. Meanwhile, the blood glucose level of the recipient mice peaked 15 min after injection. Similar results were obtained with Evans blue, used as a positive control for glucose. The synchronous fluctuation of blood glucose levels indicates that blood flow between the two mice was established successfully.

Here we describe a new method of detecting successful establishment of shared blood circulation of two parabionts through a caudal vein injection of glucose, which causes minimal damage and is not fatal to the parabionts.

考达尔静脉葡萄糖注射剂在益生菌小鼠中检测共享血液供应的建立

Parabiosis is an experimental method for surgically combining two parallel animals along the longitudinal axis of the body. We present a protocol for ...

生物学

母体叶酸和胆碱缺乏对后代健康的影响

Modifying Levels of Maternal Dietary Folic Acid or Choline to Study the Impact of Deficiencies on Offspring Health Outcomes

Maternal nutrition during pregnancy and lactation plays an important role in the neurodevelopment of offspring. One-carbon (1C) metabolism, which centers around folic acid and choline, as well as other B vitamins, plays a key role during the closure of the neural tube of the developing fetus. However, the impact of these maternal nutritional deficiencies during pregnancy on offspring health outcomes after birth remains relatively undefined. Furthermore, maternal dietary deficiencies in folic acid or choline may impact other health outcomes in offspring - making this a valuable model. This protocol aims to outline the procedure for inducing a deficiency in 1C metabolism in female mice through dietary modifications. Females are placed on diets at weaning, up to 2 months of age, for 4-6 weeks prior to mating and remain on diet throughout pregnancy and lactation. Offspring from these females can be evaluated for health outcomes. Females can be used multiple times to generate offspring, and tissues from females can be collected to measure for 1C metabolite measurements. This protocol provides an overview of how to induce maternal dietary deficiencies in folic acid or choline to study offspring health outcomes.

作者聚焦:研究母体膳食缺乏对长期后代健康结果的影响

This protocol describes the method for reducing dietary intake of folic acid or choline in female mice prior to pregnancy with the objective of investigating the impact of maternal diet on offspring health outcomes.

调整母体膳食叶酸或胆碱的水平以研究缺乏对后代健康结果的影响

Maternal nutrition during pregnancy and lactation plays an important role in the neurodevelopment of offspring. One-carbon (1C) metabolism, which centers ...

监测胞浆内单精子注射后小鼠后代的血糖

副本

摘要

探索更多视频

此视频中的章节

小鼠圆形精子细胞注射

小鼠圆形精子细胞注射

小鼠圆形精子细胞注射

用于测试胰岛素模拟化合物的蛋模型

持续药物输注系统对小鼠脑内下丘脑胰岛素信号的研究

Acclimation Prior to an Intraperitoneal Insulin Tolerance Test to Mitigate Stress-Induced Hyperglycemia in Conscious Mice

使用聚（I:C）生成妊娠中期母体免疫激活的可重复模型，以研究后代的易感性和恢复力

多囊卵巢综合征小鼠模型中肝葡萄糖产生的评价

考达尔静脉葡萄糖注射剂在益生菌小鼠中检测共享血液供应的建立

调整母体膳食叶酸或胆碱的水平以研究缺乏对后代健康结果的影响