<meta charset="utf8"></head><body>Modelo de camundongo ICSI-ET para estudar impactos de longo prazo no metabolismo da glicose

<ol>
	<li>Palermo, G., Joris, H., Devroey, P., Van Steirteghem, A. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pregnancies+after+intracytoplasmic+injection+of+single+spermatozoon+into+an+oocyte.">Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte.</a> Lancet. 340 (8810), 17-18 (1992).</li><li>Flurkey, K. M., Currer, J., Harrison, D. E., Fox, J. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vol.+III+Mouse+models+in+aging+research.+Ch.+20.">Vol. III Mouse models in aging research. Ch. 20.</a> The mouse in biomedical research (second edition). , 637-672 (2007).</li><li>Chen, M., Heilbronn, L. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+health+outcomes+of+human+offspring+conceived+by+assisted+reproductive+technologies+(art).">The health outcomes of human offspring conceived by assisted reproductive technologies (art).</a> J Dev Orig Health Dis. 8 (4), 388-402 (2017).</li><li>Heber, M. F., Ptak, G. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effects+of+assisted+reproduction+technologies+on+metabolic+health+and+diseasedagger.">The effects of assisted reproduction technologies on metabolic health and diseasedagger.</a> Biol Reprod. 104 (4), 734-744 (2021).</li><li>Ma, R. C. W., Ng, N. Y. H., Cheung, L. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assisted+reproduction+technology+and+long-term+cardiometabolic+health+in+the+offspring.">Assisted reproduction technology and long-term cardiometabolic health in the offspring.</a> PLoS Med. 18 (9), e1003724 (2021).</li><li>De Waal, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+cumulative+effect+of+assisted+reproduction+procedures+on+placental+development+and+epigenetic+perturbations+in+a+mouse+model.">The cumulative effect of assisted reproduction procedures on placental development and epigenetic perturbations in a mouse model.</a> Hum Mol Genet. 24 (24), 6975-6985 (2015).</li><li>Carlin, J., George, R., Reyes, T. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methyl+donor+supplementation+blocks+the+adverse+effects+of+maternal+high+fat+diet+on+offspring+physiology.">Methyl donor supplementation blocks the adverse effects of maternal high fat diet on offspring physiology.</a> PLoS One. 8 (5), e63549 (2013).</li><li>Hiraoka, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Piezo-icsi+for+human+oocytes.">Piezo-icsi for human oocytes.</a> J Vis Exp. (170), e60224 (2021).</li><li>Sullivan-Pyke, C. S., Senapati, S., Mainigi, M. A., Barnhart, K. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+fertilization+and+adverse+obstetric+and+perinatal+outcomes.">In vitro fertilization and adverse obstetric and perinatal outcomes.</a> Semin Perinatol. 41 (6), 345-353 (2017).</li><li>Luke, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+risk+of+birth+defects+with+conception+by+art.">The risk of birth defects with conception by art.</a> Hum Reprod. 36 (1), 116-129 (2021).</li><li>Norrman, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiovascular+disease,+obesity,+and+type+2+diabetes+in+children+born+after+assisted+reproductive+technology:+A+population-based+cohort+study.">Cardiovascular disease, obesity, and type 2 diabetes in children born after assisted reproductive technology: A population-based cohort study.</a> PLoS Med. 18 (9), e1003723 (2021).</li><li>Goldsmith, S., Mcintyre, S., Badawi, N., Hansen, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cerebral+palsy+after+assisted+reproductive+technology:+A+cohort+study.">Cerebral palsy after assisted reproductive technology: A cohort study.</a> Dev Med Child Neurol. 60 (1), 73-80 (2018).</li><li>Hansen, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intellectual+disability+in+children+conceived+using+assisted+reproductive+technology.">Intellectual disability in children conceived using assisted reproductive technology.</a> Pediatrics. 142 (6), e20181269 (2018).</li><li>Chang, R. C., Wang, H., Bedi, Y., Golding, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preconception+paternal+alcohol+exposure+exerts+sex-specific+effects+on+offspring+growth+and+long-term+metabolic+programming.">Preconception paternal alcohol exposure exerts sex-specific effects on offspring growth and long-term metabolic programming.</a> Epigenetics Chromatin. 12 (1), 9 (2019).</li><li>Grasemann, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Parental+diabetes:+The+akita+mouse+as+a+model+of+the+effects+of+maternal+and+paternal+hyperglycemia+in+wildtype+offspring.">Parental diabetes: The akita mouse as a model of the effects of maternal and paternal hyperglycemia in wildtype offspring.</a> PLoS One. 7 (11), e50210 (2012).</li></ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>1.25% avertin (2,2,2-tribromoethanol)</td>
			<td>Nanjing Aibei</td>
			<td>M2960</td>
			<td>for anesthetization</td>
		</tr>
		<tr>
			<td>Bacteriological Petri Dishes 35 x 10 mm style w/tight lid, crystal-grade virgin polystyrene, sterile</td>
			<td>BD</td>
			<td>353001</td>
			<td></td>
		</tr>
		<tr>
			<td>Bacteriological Petri Dishes 50 x 9 mm style w/tight lid, crystal-grade virgin polystyrene, sterile</td>
			<td>BD</td>
			<td>351006</td>
			<td></td>
		</tr>
		<tr>
			<td>Biosafety Cabinet</td>
			<td>ESCO</td>
			<td>class <img alt="Equation 1" src="/files/ftp_upload/66212/66212eq01.jpg" style="margin: auto;" /> BSC</td>
			<td>Aseptic operations, making culture dishes, aliquoting reagents, etc.</td>
		</tr>
		<tr>
			<td>CO2 Incubator</td>
			<td>Thermo</td>
			<td>8000DH</td>
			<td>Embryo culture</td>
		</tr>
		<tr>
			<td>Dissection Microscope</td>
			<td>Olympus</td>
			<td>SZX16</td>
			<td>Use in mouse embryo transfer</td>
		</tr>
		<tr>
			<td>Fluorinert Fc-770</td>
			<td>SIGMA</td>
			<td>F3556</td>
			<td>Fluorinert FC-770 is a thermally stable fully fluorinated liquid with high dielectric strength and resistivity, used as operating fluid</td>
		</tr>
		<tr>
			<td>handheld glucometer</td>
			<td>Roche</td>
			<td>AccuChek performa</td>
			<td>Blood glucose measurement</td>
		</tr>
		<tr>
			<td>HTF</td>
			<td>Merck</td>
			<td>MR-070-D</td>
			<td>The EmbryoMax Human Tubal Fluid (HTF) (1x), liquid designed for use with Mouse IVF is available in a 50 mL format and has been optimized and validated for Embryo Culture.</td>
		</tr>
		<tr>
			<td>Hydraulic Microinjector</td>
			<td>Eppendorf</td>
			<td>CellTram 4r Oil, 5196000030</td>
			<td>For sperm injection</td>
		</tr>
		<tr>
			<td>Inverted Microscope</td>
			<td>Nikon</td>
			<td>TI2-U</td>
			<td>Micromanipulation observation host</td>
		</tr>
		<tr>
			<td>KSOM</td>
			<td>Merck</td>
			<td>MR-020P-D</td>
			<td>(1x), Powder, w/o Phenol Red, 5 x 10 mL</td>
		</tr>
		<tr>
			<td>M2</td>
			<td>Merck</td>
			<td>MR-015-D</td>
			<td>EmbryoMax M2 Medium (1x), Liquid, with Phenol Red</td>
		</tr>
		<tr>
			<td>Micromanipulator</td>
			<td>NARISHIGE</td>
			<td>NTX-N4</td>
			<td>Micromanipulation arm</td>
		</tr>
		<tr>
			<td>mineral oil&#160;</td>
			<td>SIGMA</td>
			<td>M8410</td>
			<td>Mineral oil is suitable for use as a cover layer to control evaporation and cross-contamination in various molecular biology applications.</td>
		</tr>
		<tr>
			<td>Needle Cutter</td>
			<td>Nanjing Aibei</td>
			<td>Sutter MF-800</td>
			<td>Use for fabricating micromanipulation needles</td>
		</tr>
		<tr>
			<td>Needle Puller</td>
			<td>Nanjing Aibei</td>
			<td>Sutter model p-100</td>
			<td>Use for making micromanipulation needles</td>
		</tr>
		<tr>
			<td>Piezo Drill Trip Mouce ICSI</td>
			<td>Eppendorf</td>
			<td>5195000.087</td>
			<td>Application of ICSI injection needle.</td>
		</tr>
		<tr>
			<td>Piezoelectric Micromanipulator (Membrane Breaker)</td>
			<td>Eppendorf</td>
			<td>Eppendorf PiezoXpert</td>
			<td>Use of micro-pulses to break the zona pellucida and oolemma of the oocyte</td>
		</tr>
		<tr>
			<td>Pneumatic Microinjector</td>
			<td>Eppendorf</td>
			<td>CellTram 4r Air, 5196000013</td>
			<td>For fixing the oocyte</td>
		</tr>
		<tr>
			<td>Ready-to-use Human Chorionic Gonadotropin (Hcg)</td>
			<td>Nanjing Aibei</td>
			<td>M2520</td>
			<td>Sterilization reagent, intraperitoneal injection. 50 IU/mL</td>
		</tr>
		<tr>
			<td>Ready-to-use Pregnant Mare&#39;s Serum Gonadotropin (PMSG)</td>
			<td>Nanjing Aibei</td>
			<td>M2620</td>
			<td>Sterilization reagent, intraperitoneal injection. 50 IU/mL</td>
		</tr>
		<tr>
			<td>Stereomicroscope</td>
			<td>Olympus</td>
			<td>SZX7</td>
			<td>Oocyte retrieval and observation of embryo development</td>
		</tr>
	</tbody>
</table>

monitoring blood glucose in mouse offspring after intracytoplasmic sperm injection

Fertility issues have emerged as a major focus of medical and sociological concern, especially in modern society where declining fertility rates and the increasing severity of infertility prevalence and severity have risen to prominence. Assisted Reproductive Technology (ART) provides a wide array of possibilities to tackle these challenges, with Intracytoplasmic Sperm Injection (ICSI) commonly utilized as a therapeutic intervention.
Since Palermo reported the first successful pregnancy achieved through Intracytoplasmic Sperm Injection (ICSI) in 1992, ICSI has become a pivotal technique in Assisted Reproductive Technologies (ART)1. However, considering that ICSI has been used in clinical settings for only 30 years, a relatively brief period compared to the human lifespan, the long-term effects of ICSI, especially on offspring development, have not been extensively investigated and elucidated. At present, mice characterized by their uniform genetic background and shorter lifespan, have emerged as a widely utilized alternative model in medical research. Moreover, the mouse model can recapitulate the entire human lifespan within a compressed time frame, where one year in mice roughly corresponds to 40 years in humans2.
Over the past decade, several small-scale studies have reported that individuals conceived through ICSI may be at an increased risk of developing metabolic syndromes, such as abnormal blood sugar levels, later in life3,4. Although the evidence is not definitive, this finding has nonetheless raised serious concerns within the scientific community regarding the long-term health implications of ICSI. This situation underscores the pressing need for more rigorous assessments of ART and its long-term health consequences. Particularly in light of the limitations and ethical considerations of human studies, developing animal models that can precisely recapitulate the development of human offspring following ICSI has become increasingly crucial. In this context, the mouse ICSI-ET (Intracytoplasmic Sperm Injection-Embryo Transfer) model, owing to its capacity to mimic the human ICSI and facilitate long-term monitoring of offspring health outcomes, has become an effective tool for assessing the potential health risks of ICSI technology to offspring5.
This study aims to investigate the impact of ICSI-ET technology on a prevalent metabolic phenotype, namely offspring glucose metabolic health, by employing random blood glucose monitoring, fasting blood glucose testing, and glucose tolerance tests to assess the glucose metabolic state of mice. Random blood glucose monitoring is utilized to capture the natural fluctuations in glucose metabolism during normal physiological activities, whereas fasting blood glucose and glucose tolerance tests are employed to assess potential prediabetic states.

<meta charset="utf8"></head><body>Autor em destaque: Explorando os impactos de longo prazo na saúde da injeção intracitoplasmática de espermatozóides na prole

Monitoramento da glicose no sangue na prole de camundongos após injeção intracitoplasmática de espermatozóides

We're looking into whether the techniques used in ICSI might cause metabolic disorder in offspring. It's really important because it ensures the safety and development of assisted reproductive technology. Our research provide a solid foundation for future study enabling researchers to explore how sperm impact on embryo growth and the offspring health over time.Our team will focus on cardiovascular health and the immune system outcomes in ICSI offspring aiming to provide a comprehensive understanding of their long-term health.

Read this Scientific Article Protocol about Monitoring Blood Glucose in Mouse Offspring after Intracytoplasmic sperm injection - Embryo Transfer at JoVE.com

Human lifespan is considerably long, while mouse models can simulate the entire human lifespan in a relatively short period, with one year of mouse life ...

monitoring-blood-glucose-mouse-offspring-after-intracytoplasmic-sperm

Research

JoVE Journal

Developmental Biology

Monitoring Blood Glucose in Mouse Offspring After Intracytoplasmic Sperm Injection

332 visualizações.  Shanghai-MOST Key Laboratory of Health and Disease Genomics. Estamos investigando se as técnicas usadas na ICSI podem causar distúrbios metabólicos na prole. É muito importante porque garante a segurança e o desenvolvimento da tecnologia de reprodução assistida. Nossa pesquisa fornece uma base sólida para estudos futuros, permitindo que os pesquisadores explorem como o esperma afeta o crescimento do embrião e a saúde da prole ao longo do tempo.Nossa equipe se concentrará na saúde cardiovascular e nos...