<meta charset="utf8"></head><body>类固醇诱导的股骨头骨坏死增强的 Elastic Tape 大鼠模型

<ol>
	<li>Konarski, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Avascular+necrosis+of+femoral+head-overview+and+current+state+of+the+art.">Avascular necrosis of femoral head-overview and current state of the art.</a> Inl J Environ Res Public Health. 19 (12), 7348 (2022).</li><li>Tan, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epidemiological+study+based+on+China+osteonecrosis+of+the+femoral+head+database.">Epidemiological study based on China osteonecrosis of the femoral head database.</a> Ortho Surg. 13 (1), 153-160 (2020).</li><li>Algarni, A. D., Al Moallem, H. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+and+radiological+outcomes+of+extracorporeal+shock+wave+therapy+in+early-stage+femoral+head+osteonecrosis.">Clinical and radiological outcomes of extracorporeal shock wave therapy in early-stage femoral head osteonecrosis.</a> Adv Ortho. 2018, 7410246 (2018).</li><li>Huang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chinese+herbal+Huo-Gu+formula+for+the+treatment+of+steroid-associated+osteonecrosis+of+femoral+head:+A+14-year+follow-up+of+convalescent+SARS+patients.">Chinese herbal Huo-Gu formula for the treatment of steroid-associated osteonecrosis of femoral head: A 14-year follow-up of convalescent SARS patients.</a> J Ortho Transl. 23, 122-131 (2020).</li><li>Tomaru, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Concentrated+autologous+bone+marrow+aspirate+transplantation+versus+conservative+treatment+for+corticosteroid-associated+osteonecrosis+of+the+femoral+head+in+systemic+lupus+erythematosus.">Concentrated autologous bone marrow aspirate transplantation versus conservative treatment for corticosteroid-associated osteonecrosis of the femoral head in systemic lupus erythematosus.</a> J Rural Med. 16 (1), 1-7 (2021).</li><li>Wu, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prognostic+analysis+of+different+morphology+of+the+necrotic-viable+interface+in+osteonecrosis+of+the+femoral+head.">Prognostic analysis of different morphology of the necrotic-viable interface in osteonecrosis of the femoral head.</a> Int Ortho. 42 (1), 133-139 (2018).</li><li>Troy, K. L. R., Lundberg, H. J., Conzemius, M. G., Brown, T. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Habitual+hip+joint+activity+level+of+the+penned+EMU+(Dromaius+novaehollandie).">Habitual hip joint activity level of the penned EMU (Dromaius novaehollandie).</a> Iowa Ortho J. 27, 17 (2007).</li><li>Jiang, W., Wang, P., Wan, Y., Xin, D., Fan, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+simple+method+for+establishing+an+ostrich+model+of+femoral+head+osteonecrosis+and+collapse.">A simple method for establishing an ostrich model of femoral head osteonecrosis and collapse.</a> J Ortho Surg Res. 10, 1-10 (2015).</li><li>Murata, M., Kumagai, K., Miyata, N., Osaki, M., Shindo, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Osteonecrosis+in+stroke-prone+spontaneously+hypertensive+rats:+effect+of+glucocorticoid.">Osteonecrosis in stroke-prone spontaneously hypertensive rats: effect of glucocorticoid.</a> J Ortho Sci. 12 (3), 289-295 (2007).</li><li>Drescher, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enhanced+constriction+of+supplying+arteries-A+mechanism+of+femoral+head+Necrosis+in+Wistar+rats.">Enhanced constriction of supplying arteries-A mechanism of femoral head Necrosis in Wistar rats.</a> Ann Anat. 192 (1), 58-61 (2010).</li><li>Friedman, M. A., Zhang, Y., Wayne, J. S., Farber, C. R., Donahue, H. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single+limb+immobilization+model+for+bone+loss+from+unloading.">Single limb immobilization model for bone loss from unloading.</a> J Biomech. 83, 181-189 (2019).</li><li>Gadomski, B. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Partial+gravity+unloading+inhibits+bone+healing+responses+in+a+large+animal+model.">Partial gravity unloading inhibits bone healing responses in a large animal model.</a> J Biomech. 47 (12), 2836-2842 (2014).</li><li>Peng, P., Nie, Z., Sun, F., Peng, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glucocorticoids+induce+femoral+head+necrosis+in+rats+through+the+ROS/JNK/c-Jun+pathway.">Glucocorticoids induce femoral head necrosis in rats through the ROS/JNK/c-Jun pathway.</a> FEBS Open bio. 11 (1), 312-321 (2021).</li><li>Yan, Y. Q., Pang, Q. J., Xu, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+erythropoietin+for+precaution+of+steroid-induced+femoral+head+necrosis+in+rats.">Effects of erythropoietin for precaution of steroid-induced femoral head necrosis in rats.</a> BMC Musculoskel Dis. 19, 1-7 (2018).</li><li>Dean, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Are+rats+short-sighted?+Effects+of+stimulus+distance+and+size+on+visual+detection.">Are rats short-sighted? Effects of stimulus distance and size on visual detection.</a> Quat J Exp Psychol Sec B. 33 (2), 69-76 (1981).</li><li>Fu, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proper+mechanical+stress+promotes+femoral+head+recovery+from+steroid-induced+osteonecrosis+in+rats+through+the+OPG/RANK/RANKL+system.">Proper mechanical stress promotes femoral head recovery from steroid-induced osteonecrosis in rats through the OPG/RANK/RANKL system.</a> BMC Musculoskel Dis. 21, 281 (2020).</li><li>Wan, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+glucocorticoids+on+miRNA+expression+spectrum+of+rat+femoral+head+microcirculation+endothelial+cells.">Effect of glucocorticoids on miRNA expression spectrum of rat femoral head microcirculation endothelial cells.</a> Gene. 651, 126-133 (2018).</li><li>Alahmari, K. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+Kinesio+taping+on+cervical+proprioception+in+athletes+with+mechanical+neck+pain-a+placebo-controlled+trial.">The effect of Kinesio taping on cervical proprioception in athletes with mechanical neck pain-a placebo-controlled trial.</a> BMC Musculoskel Dis. 21, 1-9 (2020).</li><li>Shidara, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Strain-specific+differences+in+the+development+of+bone+loss+and+incidence+of+osteonecrosis+following+glucocorticoid+treatment+in+two+different+mouse+strains.">Strain-specific differences in the development of bone loss and incidence of osteonecrosis following glucocorticoid treatment in two different mouse strains.</a> J Ortho Transl. 16, 91-101 (2019).</li><li>Lv, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+model+of+traumatic+femoral+head+necrosis+in+rats+developed+by+microsurgical+technique.">A novel model of traumatic femoral head necrosis in rats developed by microsurgical technique.</a> BMC Musculoskel Dis. 23 (1), 374 (2022).</li><li>Yu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Icariin+promotes+angiogenesis+in+glucocorticoid-induced+osteonecrosis+of+femoral+heads:+In+vitro+and+in+vivo+studies.">Icariin promotes angiogenesis in glucocorticoid-induced osteonecrosis of femoral heads: In vitro and in vivo studies.</a> J Cell Mol Med. 23 (11), 7320-7330 (2019).</li><li>Kim, H. K., Morgan Bagley, S., Kostenuik, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=RANKL+inhibition:+a+novel+strategy+to+decrease+femoral+head+deformity+after+ischemic+osteonecrosis.">RANKL inhibition: a novel strategy to decrease femoral head deformity after ischemic osteonecrosis.</a> J Bone Mine Res. 21 (12), 1946-1954 (2006).</li><li>Weinstein, R. S., Jilka, R. L., Parfitt, A. M., Manolagas, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inhibition+of+osteoblastogenesis+and+promotion+of+apoptosis+of+osteoblasts+and+osteocytes+by+glucocorticoids.+Potential+mechanisms+of+their+deleterious+effects+on+bone.">Inhibition of osteoblastogenesis and promotion of apoptosis of osteoblasts and osteocytes by glucocorticoids. Potential mechanisms of their deleterious effects on bone.</a> J Clin Invest. 102 (2), 274-282 (1998).</li><li>Kapfer, S. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The effects of hyperbaric exposure on bone cell activity in the rat: implications for the pathogenesis of dysbaric osteonecrosis. , (1995).</li><li>Jiang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Puerarin+facilitates+osteogenesis+in+steroid-induced+necrosis+of+rabbit+femoral+head+and+osteogenesis+of+steroid-induced+osteocytes+via+miR-34a+upregulation.">Puerarin facilitates osteogenesis in steroid-induced necrosis of rabbit femoral head and osteogenesis of steroid-induced osteocytes via miR-34a upregulation.</a> Cytokine. 143, 155512 (2021).</li><li>Peng, P., Nie, Z., Sun, F., Peng, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glucocorticoids+induce+femoral+head+necrosis+in+rats+through+the+ROS/JNK/c-Jun+pathway.">Glucocorticoids induce femoral head necrosis in rats through the ROS/JNK/c-Jun pathway.</a> FEBS Open bio. 11 (1), 312-321 (2021).</li><li>Chen, C. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glucocorticoid-induced+loss+of+beneficial+gut+bacterial+extracellular+vesicles+is+associated+with+the+pathogenesis+of+osteonecrosis.">Glucocorticoid-induced loss of beneficial gut bacterial extracellular vesicles is associated with the pathogenesis of osteonecrosis.</a> Sci Adv. 8 (15), 8335 (2022).</li><li>Gorios Filho, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+model+study+of+ischemic+necrosis+induction+of+the+growing+femoral+head.">Experimental model study of ischemic necrosis induction of the growing femoral head.</a> Acta Ortop Bras. 30 (2), e247996 (2022).</li><li>Wilson, F. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+field+study+of+histologic+and+bacteriologic+characterization+of+femoral+head+separation+and+femoral+head+necrosis.">A field study of histologic and bacteriologic characterization of femoral head separation and femoral head necrosis.</a> Avian Dis. 64 (4), 571-581 (2020).</li><li>Fan, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparisons+of+emu+necrotic+femoral+head+micro+structure+repaired+in+two+different+methods.">Comparisons of emu necrotic femoral head micro structure repaired in two different methods.</a> Acta Acad Med Sinicae. 38 (1), 16-21 (2016).</li><li>Naito, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Femoral+head+necrosis+and+osteopenia+in+stroke-prone+spontaneously+hypertensive+rats+(SHRSPs).">Femoral head necrosis and osteopenia in stroke-prone spontaneously hypertensive rats (SHRSPs).</a> Bone. 14 (5), 745-753 (1993).</li><li>Omokawa, S., Tamai, S., Ohneda, Y., Mizumoto, S., Yamamoto, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+histopathological+study+on+the+femoral+head+necrosis+in+spontaneously+hypertensive+rats+(SHR).">A histopathological study on the femoral head necrosis in spontaneously hypertensive rats (SHR).</a> Nihon Seikeigeka Gakkai Zasshi. 66 (1), 69-82 (1992).</li></ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>15ml centrifuge tube</td>
			<td>Corning,USA</td>
			<td>430791</td>
			<td></td>
		</tr>
		<tr>
			<td>5mm stainless steel bead</td>
			<td>Gelisen,China</td>
			<td>5mm</td>
			<td></td>
		</tr>
		<tr>
			<td>Acetic acid</td>
			<td>Merck KGaA, Germany</td>
			<td>64-19-7</td>
			<td></td>
		</tr>
		<tr>
			<td>Anhydrous alcohol</td>
			<td>Merck KGaA, Germany</td>
			<td>64-17-5</td>
			<td></td>
		</tr>
		<tr>
			<td>clay</td>
			<td>Mincai stationery,China</td>
			<td>102</td>
			<td></td>
		</tr>
		<tr>
			<td>Coverslip</td>
			<td>Servicebio,China</td>
			<td>WMWD-1818</td>
			<td></td>
		</tr>
		<tr>
			<td>Flat pressure bottle 10ml</td>
			<td>BEHNCKE,China</td>
			<td>MD10ml</td>
			<td></td>
		</tr>
		<tr>
			<td>Formic acid</td>
			<td>Macklin Biochemical&#160;,China</td>
			<td>64-18-6</td>
			<td></td>
		</tr>
		<tr>
			<td>HE staining kit</td>
			<td>Solarbio,China</td>
			<td>G1120</td>
			<td></td>
		</tr>
		<tr>
			<td>HistoCore AUTOCUT</td>
			<td>Leica, Germany</td>
			<td>149AUTO00C1</td>
			<td></td>
		</tr>
		<tr>
			<td>Kinesio tape (elastic therapeutic tape)</td>
			<td>Fuluo medicine,China</td>
			<td>CL1819</td>
			<td></td>
		</tr>
		<tr>
			<td>Lipopolysaccharide</td>
			<td>Solarbio,China</td>
			<td>L8880</td>
			<td></td>
		</tr>
		<tr>
			<td>Lipopolysaccharides (LPS)</td>
			<td>Selleck,USA</td>
			<td>S7850&#160;</td>
			<td></td>
		</tr>
		<tr>
			<td>Manual carbon dioxide euthanasia box</td>
			<td>Yuyan,China</td>
			<td>LC-500-S1</td>
			<td></td>
		</tr>
		<tr>
			<td>Methylprednisolone&#160;sodium succinate,MPS</td>
			<td>AbMole,China</td>
			<td>M25573</td>
			<td></td>
		</tr>
		<tr>
			<td>MicroCT</td>
			<td>&#160;Hiscan,China</td>
			<td>&#160;Hiscan VM Pro</td>
			<td></td>
		</tr>
		<tr>
			<td>Neutral resin</td>
			<td>Beijing Zhongshan Golden Bridge Biotechnology l&#160;,China</td>
			<td>ZLI-9555</td>
			<td></td>
		</tr>
		<tr>
			<td>Paraffin</td>
			<td>Servicebio,China</td>
			<td>WGHB-319213129</td>
			<td></td>
		</tr>
		<tr>
			<td>Paraformaldehyde</td>
			<td>Servicebio,China</td>
			<td>G1101-500ML</td>
			<td></td>
		</tr>
		<tr>
			<td>Potassium chloride</td>
			<td>Macklin Biochemical&#160;,China</td>
			<td>&#160;7447-40-7</td>
			<td></td>
		</tr>
		<tr>
			<td>Slide</td>
			<td>Servicebio,China</td>
			<td>WG6012</td>
			<td></td>
		</tr>
		<tr>
			<td>Treadmill for Rats and&#160; mice</td>
			<td>Litc Life Science,USA</td>
			<td>801</td>
			<td></td>
		</tr>
		<tr>
			<td>Xylene</td>
			<td>Macklin Biochemical&#160;,China</td>
			<td>&#160;1330-20-7</td>
			<td></td>
		</tr>
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</table>

tension-free weight-bearing model of steroid-induced osteonecrosis of femoral head in rats

The administration of glucocorticoids is the most common risk factor for non-traumatic osteonecrosis of the femoral head (ONFH)1. Numerous pieces of evidence suggest that, in addition to glucocorticoids, the pressure load on the hip joint in patients is also associated with the occurrence of ONFH. Factors such as body weight and physical labor intensity are recognized as risk factors for ONFH2. Multiple clinical studies have demonstrated a close relationship between hip joint loading conditions and the timing and incidence of joint replacement3,4,5,6. Therefore, establishing a model that reflects the relationship between load bearing and steroid-induced osteonecrosis of the femoral head (SONFH) is important for a comprehensive investigation of this condition.
Large bipedal birds, such as ostriches and emus, serve as good models to simulate hip joint stress, resembling human leg loads7,8. However, maintaining large bird species is challenging, and the associated research costs are high. Spontaneously hypertensive rat models9,10 can exhibit higher rates of ONFH, but the compartment pressure load in the marrow generated by spontaneous hypertension differs significantly from mechanical pressure and is unsuitable for studying the impact of mechanical pressure on SONFH.
Small animal models are commonly used in research on SONFH. However, quadrupedal reptiles have lower hip joint stress, and their hip models cannot simulate the biomechanical environment of human hip joints during bipedal walking. Single limb immobilization models11 and partial unloading models12 are common, but both reduce limb loading. As humans are bipedal organisms with substantial lower limb loads while standing and walking, reducing the load in these models diminishes the connection between animal models and human diseases.
This study aims to establish a simple and cost-effective model for weight-bearing training to investigate the impact of weight-bearing training on steroid-induced osteonecrosis of the femoral head in rats. Currently, rat models have been used to study steroid-induced osteonecrosis of the femoral head13,14, but there is still no model that can provide long-term, safe fixation with minimal disruption to movement, which is also relatively simple and inexpensive. In this study, a high-adhesive fixation material is applied, adopting tension-free fixation, which maintains the rats&#39; mobility and reduces the suffering and even death caused by improper fixation.

<meta charset="utf8"></head><body>作者聚焦：开发用于负重干预的大鼠模型以研究股骨头骨坏死

类固醇诱导大鼠股骨头骨坏死的无张力负重模型

We primarily developed the rat model for weight-bearing interventions to investigate the efforts of weight-bearing exercise on osteonecrosis of the femoral head. Currently, most of the models are non-weight-bearing models. However, the progressions of the osteonecrosis in the femoral head may be associated with weight-bearing exercises.It's actually very difficult to secure weights onto rats as most method severe restricted their moment, thereby failing to achieve their purpose of weight-bearing exercises.

Unlike humans, rats are animals that walk on all fours, while humans are bipedal animals that stand, and the hips are subjected to tremendous pressure ...

tension-free-weight-bearing-model-steroid-induced-osteonecrosis

Research

JoVE Journal

Medicine

Tension-Free Weight-Bearing Model of Steroid-Induced Osteonecrosis of Femoral Head in Rats

299 次观看.  Beijing University of Chinese Medicine. 我们主要开发了用于负重干预的大鼠模型，以研究负重运动对股骨头骨坏死的作用。目前，大多数型号都是非负重型号。然而，股骨头骨坏死的进展可能与负重运动有关。实际上，将重物固定在大鼠身上非常困难，因为大多数方法都严重限制了它们的力矩，从而无法达到负重锻炼的目的。