<meta charset="utf8"></head><body>Steroid ile İndüklenen Femur Başı Osteonekroz Geliştirme Elastik Bant Sıçan Modeli

<ol>
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A., Zhang, Y., Wayne, J. S., Farber, C. R., Donahue, H. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single+limb+immobilization+model+for+bone+loss+from+unloading.">Single limb immobilization model for bone loss from unloading.</a> J Biomech. 83, 181-189 (2019).</li><li>Gadomski, B. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Partial+gravity+unloading+inhibits+bone+healing+responses+in+a+large+animal+model.">Partial gravity unloading inhibits bone healing responses in a large animal model.</a> J Biomech. 47 (12), 2836-2842 (2014).</li><li>Peng, P., Nie, Z., Sun, F., Peng, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glucocorticoids+induce+femoral+head+necrosis+in+rats+through+the+ROS/JNK/c-Jun+pathway.">Glucocorticoids induce femoral head necrosis in rats through the ROS/JNK/c-Jun pathway.</a> FEBS Open bio. 11 (1), 312-321 (2021).</li><li>Yan, Y. Q., Pang, Q. J., Xu, R. 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A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+Kinesio+taping+on+cervical+proprioception+in+athletes+with+mechanical+neck+pain-a+placebo-controlled+trial.">The effect of Kinesio taping on cervical proprioception in athletes with mechanical neck pain-a placebo-controlled trial.</a> BMC Musculoskel Dis. 21, 1-9 (2020).</li><li>Shidara, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Strain-specific+differences+in+the+development+of+bone+loss+and+incidence+of+osteonecrosis+following+glucocorticoid+treatment+in+two+different+mouse+strains.">Strain-specific differences in the development of bone loss and incidence of osteonecrosis following glucocorticoid treatment in two different mouse strains.</a> J Ortho Transl. 16, 91-101 (2019).</li><li>Lv, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+model+of+traumatic+femoral+head+necrosis+in+rats+developed+by+microsurgical+technique.">A novel model of traumatic femoral head necrosis in rats developed by microsurgical technique.</a> BMC Musculoskel Dis. 23 (1), 374 (2022).</li><li>Yu, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Icariin+promotes+angiogenesis+in+glucocorticoid-induced+osteonecrosis+of+femoral+heads:+In+vitro+and+in+vivo+studies.">Icariin promotes angiogenesis in glucocorticoid-induced osteonecrosis of femoral heads: In vitro and in vivo studies.</a> J Cell Mol Med. 23 (11), 7320-7330 (2019).</li><li>Kim, H. K., Morgan Bagley, S., Kostenuik, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=RANKL+inhibition:+a+novel+strategy+to+decrease+femoral+head+deformity+after+ischemic+osteonecrosis.">RANKL inhibition: a novel strategy to decrease femoral head deformity after ischemic osteonecrosis.</a> J Bone Mine Res. 21 (12), 1946-1954 (2006).</li><li>Weinstein, R. S., Jilka, R. L., Parfitt, A. M., Manolagas, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inhibition+of+osteoblastogenesis+and+promotion+of+apoptosis+of+osteoblasts+and+osteocytes+by+glucocorticoids.+Potential+mechanisms+of+their+deleterious+effects+on+bone.">Inhibition of osteoblastogenesis and promotion of apoptosis of osteoblasts and osteocytes by glucocorticoids. Potential mechanisms of their deleterious effects on bone.</a> J Clin Invest. 102 (2), 274-282 (1998).</li><li>Kapfer, S. 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D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+field+study+of+histologic+and+bacteriologic+characterization+of+femoral+head+separation+and+femoral+head+necrosis.">A field study of histologic and bacteriologic characterization of femoral head separation and femoral head necrosis.</a> Avian Dis. 64 (4), 571-581 (2020).</li><li>Fan, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparisons+of+emu+necrotic+femoral+head+micro+structure+repaired+in+two+different+methods.">Comparisons of emu necrotic femoral head micro structure repaired in two different methods.</a> Acta Acad Med Sinicae. 38 (1), 16-21 (2016).</li><li>Naito, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Femoral+head+necrosis+and+osteopenia+in+stroke-prone+spontaneously+hypertensive+rats+(SHRSPs).">Femoral head necrosis and osteopenia in stroke-prone spontaneously hypertensive rats (SHRSPs).</a> Bone. 14 (5), 745-753 (1993).</li><li>Omokawa, S., Tamai, S., Ohneda, Y., Mizumoto, S., Yamamoto, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+histopathological+study+on+the+femoral+head+necrosis+in+spontaneously+hypertensive+rats+(SHR).">A histopathological study on the femoral head necrosis in spontaneously hypertensive rats (SHR).</a> Nihon Seikeigeka Gakkai Zasshi. 66 (1), 69-82 (1992).</li></ol>

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			<td>15ml centrifuge tube</td>
			<td>Corning,USA</td>
			<td>430791</td>
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			<td>5mm stainless steel bead</td>
			<td>Gelisen,China</td>
			<td>5mm</td>
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			<td>Acetic acid</td>
			<td>Merck KGaA, Germany</td>
			<td>64-19-7</td>
			<td></td>
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			<td>Anhydrous alcohol</td>
			<td>Merck KGaA, Germany</td>
			<td>64-17-5</td>
			<td></td>
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			<td>clay</td>
			<td>Mincai stationery,China</td>
			<td>102</td>
			<td></td>
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			<td>Coverslip</td>
			<td>Servicebio,China</td>
			<td>WMWD-1818</td>
			<td></td>
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			<td>Flat pressure bottle 10ml</td>
			<td>BEHNCKE,China</td>
			<td>MD10ml</td>
			<td></td>
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			<td>Formic acid</td>
			<td>Macklin Biochemical&#160;,China</td>
			<td>64-18-6</td>
			<td></td>
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			<td>HE staining kit</td>
			<td>Solarbio,China</td>
			<td>G1120</td>
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			<td>HistoCore AUTOCUT</td>
			<td>Leica, Germany</td>
			<td>149AUTO00C1</td>
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			<td>Kinesio tape (elastic therapeutic tape)</td>
			<td>Fuluo medicine,China</td>
			<td>CL1819</td>
			<td></td>
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			<td>Lipopolysaccharide</td>
			<td>Solarbio,China</td>
			<td>L8880</td>
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			<td>Lipopolysaccharides (LPS)</td>
			<td>Selleck,USA</td>
			<td>S7850&#160;</td>
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			<td>Manual carbon dioxide euthanasia box</td>
			<td>Yuyan,China</td>
			<td>LC-500-S1</td>
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			<td>Methylprednisolone&#160;sodium succinate,MPS</td>
			<td>AbMole,China</td>
			<td>M25573</td>
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			<td>&#160;Hiscan,China</td>
			<td>&#160;Hiscan VM Pro</td>
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			<td>Neutral resin</td>
			<td>Beijing Zhongshan Golden Bridge Biotechnology l&#160;,China</td>
			<td>ZLI-9555</td>
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			<td>Paraffin</td>
			<td>Servicebio,China</td>
			<td>WGHB-319213129</td>
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			<td>Servicebio,China</td>
			<td>G1101-500ML</td>
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			<td>Macklin Biochemical&#160;,China</td>
			<td>&#160;7447-40-7</td>
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			<td>Treadmill for Rats and&#160; mice</td>
			<td>Litc Life Science,USA</td>
			<td>801</td>
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			<td>Xylene</td>
			<td>Macklin Biochemical&#160;,China</td>
			<td>&#160;1330-20-7</td>
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tension-free weight-bearing model of steroid-induced osteonecrosis of femoral head in rats

<meta charset="utf8"></head><body>Yazar Spotlight: Femur Başının Osteonekrozunu Araştırmak İçin Ağırlık Taşıma Müdahalesi için Bir Sıçan Modeli Geliştirme

Sıçanlarda Femur Başının Steroid ile İndüklenen Osteonekrozunun Gerilimsiz Ağırlık Taşıma Modeli

Unlike humans, rats are animals that walk on all fours, while humans are bipedal animals that stand, and the hips are subjected to tremendous pressure ...

tension-free-weight-bearing-model-steroid-induced-osteonecrosis

Research

JoVE Journal

Medicine

Tension-Free Weight-Bearing Model of Steroid-Induced Osteonecrosis of Femoral Head in Rats

392 Görüntüleme.  Beijing University of Chinese Medicine. Öncelikle femur başı osteonekrozu üzerinde ağırlık taşıma egzersizinin çabalarını araştırmak için ağırlık taşıma girişimleri için sıçan modelini geliştirdik. Şu anda, modellerin çoğu ağırlık taşımayan modellerdir. Bununla birlikte, femur başındaki osteonekrozun ilerlemesi ağırlık taşıma egzersizleri ile ilişkili olabilir.Farelere ağırlık sabitlemek aslında çok zordur, çünkü çoğu yöntem momentlerini ci...

Video: Yazar Spotlight: Femur Başının Osteonekrozunu Araştırmak İçin Ağırlık Taşıma Müdahalesi için Bir Sıçan Modeli Geliştirme

A Simple Critical-sized Femoral Defect Model in Mice

While bone has a remarkable capacity for regeneration, serious bone trauma often results in damage that does not properly heal. In fact, one tenth of all limb bone fractures fail to heal completely due to the extent of the trauma, disease, or age of the patient. Our ability to improve bone regenerative strategies is critically dependent on the ability to mimic serious bone trauma in test animals, but the generation and stabilization of large bone lesions is technically challenging. In most cases, serious long bone trauma is mimicked experimentally by establishing a defect that will not naturally heal. This is achieved by complete removal of a bone segment that is larger than 1.5 times the diameter of the bone cross-section. The bone is then stabilized with a metal implant to maintain proper orientation of the fracture edges and allow for mobility. 
Due to their small size and the fragility of their long bones, establishment of such lesions in mice are beyond the capabilities of most research groups. As such, long bone defect models are confined to rats and larger animals. Nevertheless, mice afford significant research advantages in that they can be genetically modified and bred as immune-compromised strains that do not reject human cells and tissue.
Herein, we demonstrate a technique that facilitates the generation of a segmental defect in mouse femora using standard laboratory and veterinary equipment. With practice, fabrication of the fixation device and surgical implantation is feasible for the majority of trained veterinarians and animal research personnel. Using example data, we also provide methodologies for the quantitative analysis of bone healing for the model.

Animal models are frequently employed to mimic serious bone injury in biomedical research. Due to their small size, establishment of stabilized bone lesions in mice are beyond the capabilities of most research groups. Herein, we describe a simple method for establishing and analyzing experimental femoral defects in mice.

Farelerde Basit Kritik ölçekli Femoral Hata Modeli

While bone has a remarkable capacity for regeneration, serious bone trauma often results in damage that does not properly heal. In fact, one tenth of all ...

Tıp

A Repetitive Concussive Head Injury Model in Mice

Despite the concussion/ mild traumatic brain injury (mTBI) being the most frequent occurrence of traumatic brain injury, there is still a lack of knowledge on the injury and its effects. To develop a better understanding of concussions, animals are often used because they provide a controlled, rigorous, and efficient model. Studies have adapted traditional animal models to perform mTBI to stimulate mild injury severity by changing the injury parameters. These models have been used because they can produce morphologically similar brain injuries to the clinical condition and provide a spectrum of injury severities. However, they are limited in their ability to present the identical features of injuries in patients. Using a traditional impact system, a repetitive concussive injury (rCHI) model can induce mild to moderate human-like concussion. The injury degree can be determined by measuring the period of loss of consciousness (LOC) with a sign of a transient termination of breathing. The rCHI model is beneficial to use for its accuracy and simplicity in determining mTBI effects and potential treatments.

Concussion presents the most common type of traumatic brain injury. Therefore, a repetitive concussive animal model, which replicates the important features of an injury in patients, may provide a means to study concussion in a rigorous, controlled, and efficient manner.

Farelerde Bir Tekrarlanan Concussive Kafa Travması Modeli

Despite the concussion/ mild traumatic brain injury (mTBI) being the most frequent occurrence of traumatic brain injury, there is still a lack of ...

Ultrasound Assessment of Flow-Mediated Dilation of the Brachial and Superficial Femoral Arteries in Rats

Arterial vasodilation to increases in wall shear rate is indicative of vascular endothelial function. In humans, the non-invasive measurement of endothelial function can be achieved by employing the flow-mediated dilation technique, typically performed in the brachial or superficial femoral artery. Briefly, a blood pressure cuff placed distal to an ultrasound probe is inflated to a suprasystolic pressure, which results in limb ischemia. After 5 min of occlusion the cuff is deflated, resulting in reactive hyperemia and increases in wall shear rate that signal vasodilatory molecules to be released from the endothelium eliciting vasodilation. Despite the thousands of studies performing flow-mediated dilation in humans, surprisingly, no studies have performed this technique non-invasively in living rats. Considering the recent shift in focus to translational research, the establishment of guidelines for non-invasive measurement of flow-mediated dilation in rats and other rodents would be extremely valuable. In the following article, a protocol is presented for the non-invasive measurement of flow-mediated dilation in brachial and superficial femoral arteries of rats, as those sites are most commonly measured in humans.

Non-invasive assessment of endothelial function in humans can be determined by the flow-mediated dilation technique. Although thousands of studies have used this technique, no study has performed this technique non-invasively in rats. The following article describes non-invasive measurement of flow-mediated dilation in the brachial and superficial femoral arteries of rats.

Flow-Aracılı brakiyal dilatasyonu ve Sıçanlarda Yüzeyel Femoral Arter Ultrason Değerlendirmesi

Arterial vasodilation to increases in wall shear rate is indicative of vascular endothelial function. In humans, the non-invasive measurement of ...

Preparation of Decellularized Kidney Scaffolds in Rats

Tissue engineering is a cutting-edge discipline in biomedicine. Cell culture techniques can be applied for regeneration of functional tissues and organs to replace diseased or damaged organs. Scaffolds are needed to facilitate the generation of three-dimensional organs or tissues using differentiated stem cells in vivo. In this report, we describe a novel method for developing vascularized scaffolds using decellularized rat kidneys. Eight-week-old Sprague-Dawley rats were used in this study, and heparin was injected into the heart to facilitate flow into the renal vessels, allowing heparin to perfuse into the renal vessels. The abdominal cavity was opened, and the left kidney was collected. The collected kidneys were perfused for 9 h using detergents, such as Triton X-100 and sodium dodecyl sulfate, to decellularize the tissue. Decellularized kidney scaffolds were then gently washed with 1% penicillin/streptomycin and heparin to remove cellular debris and chemical residues. Transplantation of stem cells with the decellularized vascular scaffolds is expected to facilitate the generation of new organs. Thus, the vascularized scaffolds may provide a foundation for tissue engineering of organ grafts in the future.

This protocol introduces a method to develop a scaffold using decellularized rat kidneys. The protocol includes decellularization and recellularization processes to confirm bioavailability. Decellularization is performed using Triton X-100 and sodium dodecyl sulfate.

Sıçanlarda Hücreden Sönmemiş Böbrek İskelelerinin Hazırlanması

Tissue engineering is a cutting-edge discipline in biomedicine. Cell culture techniques can be applied for regeneration of functional tissues and organs ...

In Vivo Quantification of Hip Arthrokinematics during Dynamic Weight-bearing Activities using Dual Fluoroscopy

Several hip pathologies have been attributed to abnormal morphology with an underlying assumption of aberrant biomechanics. However, structure-function relationships at the joint level remain challenging to quantify due to difficulties in accurately measuring dynamic joint motion. The soft tissue artifact errors inherent in optical skin marker motion capture are exacerbated by the depth of the hip joint within the body and the large mass of soft tissue surrounding the joint. Thus, the complex relationship between bone shape and hip joint kinematics is more difficult to study accurately than in other joints. Herein, a protocol incorporating computed tomography (CT) arthrography, three-dimensional (3D) reconstruction of volumetric images, dual fluoroscopy, and optical motion capture to accurately measure the dynamic motion of the hip joint is presented. The technical and clinical studies that have applied dual fluoroscopy to study form-function relationships of the hip using this protocol are summarized, and the specific steps and future considerations for data acquisition, processing, and analysis are described.

In Vivo Quantification of Hip Arthrokinematics during Dynamic Weight-bearing Activities using Dual Fluoroscopy

Dual fluoroscopy accurately captures in vivo dynamic motion of human joints, which can be visualized relative to reconstructed anatomy (e.g., arthrokinematics). Herein, a detailed protocol to quantify hip arthrokinematics during weight-bearing activities of daily living is presented, including the integration of dual fluoroscopy with traditional skin marker motion capture.

In Vivo Çift Floroskopi Kullanılarak Dinamik Ağırlık Taşıyan Faaliyetler Sırasında Kalça Arthrokinematics Nicelliği

Several hip pathologies have been attributed to abnormal morphology with an underlying assumption of aberrant biomechanics. However, structure-function ...

Augmented Reality Navigation-Guided Core Decompression for Osteonecrosis of Femoral Head

Osteonecrosis of the femoral head (ONFH) is a common joint disease in young and middle-aged patients, which seriously burdens their lives and work. For early-stage ONFH, core decompression surgery is a classical and effective hip preservation therapy. In traditional procedures of core decompression with Kirschner wire, there are still many problems such as X-ray exposure, repeated puncture verification, and damage to normal bone tissue. The blindness of the puncture process and the inability to provide real-time visualization are crucial reasons for these problems.
To optimize this procedure, our team developed an intraoperative navigation system on the basis of augmented reality (AR) technology. This surgical system can intuitively display the anatomy of the surgical areas and render preoperative images and virtual needles to intraoperative video in real-time. With the guide of the navigation system, surgeons can accurately insert Kirschner wires into the targeted lesion area and minimize the collateral damage. We conducted 10 cases of core decompression surgery with this system. The efficiency of positioning and fluoroscopy is greatly improved compared to the traditional procedures, and the accuracy of puncture is also guaranteed.

Augmented reality technology was applied to core decompression for osteonecrosis of the femoral head to realize real-time visualization of this surgical procedure. This method can effectively improve the safety and precision of core decompression.

Femur Başı Osteonekrozu için Artırılmış Gerçeklik Navigasyon Rehberliğinde Çekirdek Dekompresyonu

Osteonecrosis of the femoral head (ONFH) is a common joint disease in young and middle-aged patients, which seriously burdens their lives and work. For ...

Orthopedic Robot-Assisted Femoral Neck System in the Treatment of Femoral Neck Fracture

Cannulated screw fixation is the main therapy for femoral neck fractures, especially in young patients. The traditional surgical procedure uses C-arm fluoroscopy to place the screw freehand and requires several guide wire adjustments, which increases the operation time and radiation exposure. Repeated drilling can also cause damage to the blood supply and bone quality of the femoral neck, which can be followed by complications such as screw loosening, nonunion, and femoral head necrosis. In order to make fixation more precise and reduce the incidence of complications, our team applied robot-assisted orthopedic surgery for screw placement using the femoral neck system to modify the traditional procedure. This protocol introduces how to import a patient's X-ray information into the system, how to perform screw path planning in software, and how the robotic arm assists in screw placement. Using this method, the surgeons can place the screw successfully the first time, improve the accuracy of the procedure, and avoid radiation exposure. The whole protocol includes the diagnosis of femoral neck fracture; the collection of intraoperative X-ray images; screw path planning in the software; precise placement of the screw under the assistance of the robotic arm by the surgeon; and verification of the implant placement.

This article introduces a method of robot-assisted orthopedic surgery for screw placement during the treatment of femoral neck fracture using the femoral neck system, which allows for more accurate screw placement, improved surgical efficiency, and fewer complications.

Femur Boyun Kırığı Tedavisinde Ortopedik Robot Yardımlı Femur Boyun Sistemi

Cannulated screw fixation is the main therapy for femoral neck fractures, especially in young patients. The traditional surgical procedure uses C-arm ...

ACLR Hastaları için Rehabilitasyon Tedavi Planı

Early Weight-Bearing Rehabilitation Protocol After Anterior Cruciate Ligament Reconstruction

Anterior cruciate ligament (ACL) injury is one of the common sports injuries. Anterior cruciate ligament reconstruction (ACLR) is the mainstream treatment for ACL injury, aiming to regain normal anatomical structure and stability of the knee joint and promote the patient's return to sports. Under the guidance of the concept of enhanced recovery after surgery, early weight-bearing rehabilitation (EWB) is an important factor affecting patient function and quality of life. However, there is no consensus on whether EWB rehabilitation can be performed after ACL surgery. 
This study aims to explore the safety and feasibility of EWB after ACL surgery. The study implemented a gradual EWB rehabilitation program in the experimental group, including weight-shifting training, balance training, and gait training on the affected lower limb, and assessed wound healing and stability of the knee joint. The study found that EWB after ACLR is safe and feasible. EWB rehabilitation not only does not pose a negative effect on the patient's knee pain, swelling, wound healing, and stability, but also helps to improve knee active flexion and quality of life faster and better. The EWB program in this study is simple, safe, and effective, and it provides strong theoretical guidance and practical demonstration for accelerated rehabilitation after ACLR.

Yazar Gündemi: Ön Çapraz Bağ Rekonstrüksiyonunu Takiben Rehabilitasyonda Ameliyat Sonrası Gelişmiş İyileşme Konseptinin Uygulanması

Based on the safety and feasibility, this article presents an early weight-bearing rehabilitation protocol after anterior cruciate ligament reconstruction. The protocol is clear and easy to operate, which is helpful to promote its use in clinical practice and accelerate the functional recovery of patients.

Ön Çapraz Bağ Rekonstrüksiyonu Sonrası Erken Ağırlık Taşıyan Rehabilitasyon Protokolü

Anterior cruciate ligament (ACL) injury is one of the common sports injuries. Anterior cruciate ligament reconstruction (ACLR) is the mainstream treatment ...