in-vitro-reconstitution-self-organizing-protein-patterns-on-supported

<em>In Vitro</em> Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers

Many aspects of the fundamental spatiotemporal organization of cells are governed by reaction-diffusion type systems. In vitro reconstitution of such ...

Max Planck Institute of Biochemistry

Patterns formed by reaction and diffusion are the foundation for many phenomena in biology. However, the experimental study of reaction-diffusion (R-D) systems has so far been dominated by chemical oscillators, for which many tools are available. In this work, we developed a photoswitch for the Min system of Escherichia coli, a versatile biological in vitro R-D system consisting of the antagonistic proteins MinD and MinE. A MinE-derived peptide of 19 amino acids was covalently modified with a photoisomerizable crosslinker based on azobenzene to externally control peptide-mediated depletion of MinD from the membrane. In addition to providing an on-off switch for pattern formation, we achieve frequency-locked resonance with a precise 2D spatial memory, thus allowing new insights into Min protein action on the membrane. Taken together, we provide a tool to study phenomena in pattern formation using biological agents.

Philipp Glock

Department of Cellular and Molecular Biophysics

Max Planck Institute of Biochemistry

In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers

Philipp Glock

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Cellular and Molecular Biophysics

Max Planck Institute of Biochemistry

In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers

Department of Cellular and Molecular Biophysics