Petra Schwille

Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells.

Importin 8 is a gene silencing factor that targets argonaute proteins to distinct mRNAs.

Minimal systems to study membrane-cytoskeleton interactions.

Towards a bottom-up reconstitution of bacterial cell division.

Reconstitution of pole-to-pole oscillations of min proteins in microengineered polydimethylsiloxane compartments.

Myosin motors fragment and compact membrane-bound actin filaments.

Photoconversion of bodipy-labeled lipid analogues.

Lateral membrane diffusion modulated by a minimal actin cortex.

The design of MACs (minimal actin cortices).

Propagation of MinCDE waves on free-standing membranes.

MinC, MinD, and MinE drive counter-oscillation of early-cell-division proteins prior to Escherichia coli septum formation.

Bacterial cell division: a swirling ring to rule them all?

MinCDE exploits the dynamic nature of FtsZ filaments for its spatial regulation.

Asymmetric supported lipid bilayer formation via methyl-β-cyclodextrin mediated lipid exchange: influence of asymmetry on lipid dynamics and phase behavior.

ESCRT-III mediated cell division in Sulfolobus acidocaldarius - a reconstitution perspective.

Surface topology assisted alignment of Min protein waves.

Fluorescence fluctuation microscopy: a diversified arsenal of methods to investigate molecular dynamics inside cells.

Reconstitution of self-organizing protein gradients as spatial cues in cell-free systems.

Toward Spatially Regulated Division of Protocells: Insights into the E. coli Min System from in Vitro Studies.

Cytoskeletal pinning controls phase separation in multicomponent lipid membranes.

Amphipathic DNA origami nanoparticles to scaffold and deform lipid membrane vesicles.

Adaptive lipid packing and bioactivity in membrane domains.

FtsZ Polymers Tethered to the Membrane by ZipA Are Susceptible to Spatial Regulation by Min Waves.

Jump-starting life? Fundamental aspects of synthetic biology.

Introducing a fluorescence-based standard to quantify protein partitioning into membranes.

Diffusion coefficients and dissociation constants of enhanced green fluorescent protein binding to free standing membranes.

Pattern formation on membranes and its role in bacterial cell division.

DNA Nanostructures on Membranes as Tools for Synthetic Biology.

Single Particle Plasmon Sensors as Label-Free Technique To Monitor MinDE Protein Wave Propagation on Membranes.

Protein Patterns and Oscillations on Lipid Monolayers and in Microdroplets.

Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes.

Optical Control of Lipid Rafts with Photoswitchable Ceramides.

In vitro Reconstitution of a Membrane Switch Mechanism for the Polarity Protein LGL.

Transport efficiency of membrane-anchored kinesin-1 motors depends on motor density and diffusivity.

Diffusion of Single-Pass Transmembrane Receptors: From the Plasma Membrane into Giant Liposomes.

Control of lipid domain organization by a biomimetic contractile actomyosin cortex.

Reconstitution of Protein Dynamics Involved in Bacterial Cell Division.

Large-scale modulation of reconstituted Min protein patterns and gradients by defined mutations in MinE's membrane targeting sequence.

Revolving around constriction by ESCRT-III.

Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks.

Biology and the art of abstraction.

High-Speed Atomic Force Microscopy Reveals the Inner Workings of the MinDE Protein Oscillator.

Optical Control of a Biological Reaction-Diffusion System.

Membrane sculpting by curved DNA origami scaffolds.

There and back again: from the origin of life to single molecules.

FCS Analysis of Protein Mobility on Lipid Monolayers.

Reverse and forward engineering of protein pattern formation.

Quantifying Reversible Surface Binding via Surface-Integrated Fluorescence Correlation Spectroscopy.

MinE conformational switching confers robustness on self-organized Min protein patterns.

Photophysical Behavior of mNeonGreen, an Evolutionarily Distant Green Fluorescent Protein.

MaxSynBio - Avenues towards creating cells from the bottom up.

Liposomes and polymersomes: a comparative review towards cell mimicking.

Control of Membrane Binding and Diffusion of Cholesteryl-Modified DNA Origami Nanostructures by DNA Spacers.

The MinDE system is a generic spatial cue for membrane protein distribution in vitro.

Beating Vesicles: Encapsulated Protein Oscillations Cause Dynamic Membrane Deformations.

Light-Induced Printing of Protein Structures on Membranes in Vitro.

Membrane association and remodeling by intraflagellar transport protein IFT172.

In vitro reconstitution of the bacterial cytoskeleton: expected and unexpected new insights.

Plasmonic Nanosensors Reveal a Height Dependence of MinDE Protein Oscillations on Membrane Features.

Photo-Induced Depletion of Binding Sites in DNA-PAINT Microscopy.

Myosin-II activity generates a dynamic steady state with continuous actin turnover in a minimal actin cortex.

Single Particle Tracking and Super-Resolution Imaging of Membrane-Assisted Stop-and-Go Diffusion and Lattice Assembly of DNA Origami.

More from less - bottom-up reconstitution of cell biology.

Optical manipulation of sphingolipid biosynthesis using photoswitchable ceramides.

124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics.

Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification.

Temperature-sensitive protein expression in protocells.

Manfred Eigen (1927-2019).

Synthetic cell division via membrane-transforming molecular assemblies.

Design of Sealable Custom-Shaped Cell Mimicries Based on Self-Assembled Monolayers on CYTOP Polymer.

Bottom-up synthetic biology: reconstitution in space and time.

Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes.

The E. coli MinCDE system in the regulation of protein patterns and gradients.

Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System.

Heated gas bubbles enrich, crystallize, dry, phosphorylate and encapsulate prebiotic molecules.

Toward Absolute Molecular Numbers in DNA-PAINT.

An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions.

Design of biochemical pattern forming systems from minimal motifs.

Shaping Giant Membrane Vesicles in 3D-Printed Protein Hydrogel Cages.

Local Self-Enhancement of MinD Membrane Binding in Min Protein Pattern Formation.

Reversible membrane deformations by straight DNA origami filaments.

Symmetry Breaking and Emergence of Directional Flows in Minimal Actomyosin Cortices.

The speed of FtsZ treadmilling is tightly regulated by membrane binding.

Cytoskeletal and Actin-Based Polymerization Motors and Their Role in Minimal Cell Design.

Cell-Free Protein Synthesis and Its Perspectives for Assembling Cells from the Bottom-Up.

Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin.

FtsZ Reorganization Facilitates Deformation of Giant Vesicles in Microfluidic Traps*.

Fine-Tuning Protein Self-Organization by Orthogonal Chemo-Optogenetic Tools.

Non-Equilibrium Large-Scale Membrane Transformations Driven by MinDE Biochemical Reaction Cycles.

Probing Biomolecular Interactions by a Pattern-Forming Peptide-Conjugate Sensor.

Division in synthetic cells.

Protein Reconstitution Inside Giant Unilamellar Vesicles.

De novo design of a reversible phosphorylation-dependent switch for membrane targeting.

Reconstitution of contractile actomyosin rings in vesicles.

Increasing MinD's Membrane Affinity Yields Standing Wave Oscillations and Functional Gradients on Flat Membranes.

FtsZ induces membrane deformations via torsional stress upon GTP hydrolysis.

Membrane-coated 3D architectures for bottom-up synthetic biology.

Molecular-scale visualization of sarcomere contraction within native cardiomyocytes.

Tracking single particles for hours via continuous DNA-mediated fluorophore exchange.

Hydration Layer of Only a Few Molecules Controls Lipid Mobility in Biomimetic Membranes.

Self-organized protein patterns: The MinCDE and ParABS systems.

Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture.

Mass-sensitive particle tracking to elucidate the membrane-associated MinDE reaction cycle.

Hidden protein functions and what they may teach us.

Design Features to Accelerate the Higher-Order Assembly of DNA Origami on Membranes.

CTP-controlled liquid-liquid phase separation of ParB.