Anastassis Perrakis

Ptprj is a candidate for the mouse colon-cancer susceptibility locus Scc1 and is frequently deleted in human cancers.

Crystal structure of the retinoblastoma tumor suppressor protein bound to E2F and the molecular basis of its regulation.

Structures of Escherichia coli DNA mismatch repair enzyme MutS in complex with different mismatches: a common recognition mode for diverse substrates.

Crystal structure of the targeting endonuclease of the human LINE-1 retrotransposon.

Towards complete validated models in the next generation of ARP/wARP.

Inhibition of autotaxin by lysophosphatidic acid and sphingosine 1-phosphate.

Modeling and experimental validation of the binary complex of the plectin actin-binding domain and the first pair of fibronectin type III (FNIII) domains of the beta4 integrin.

Structure and E3-ligase activity of the Ring-Ring complex of polycomb proteins Bmi1 and Ring1b.

Assessment of automatic ligand building in ARP/wARP.

The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase.

Determinants for DNA target structure selectivity of the human LINE-1 retrotransposon endonuclease.

ARP/wARP and molecular replacement: the next generation.

RNA-binding protein Dnd1 inhibits microRNA access to target mRNA.

A knowledge-driven approach for crystallographic protein model completion.

Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7.

Insights into the DNA cleavage mechanism of human LINE-1 retrotransposon endonuclease.

"Conditional Restraints": Restraining the Free Atoms in ARP/wARP.

Use of acetylcholine binding protein in the search for novel alpha7 nicotinic receptor ligands. In silico docking, pharmacological screening, and X-ray analysis.

UV-induced ligand exchange in MHC class I protein crystals.

Class I major histocompatibility complexes loaded by a periodate trigger.

Ca2+ regulation in the Na+/Ca2+ exchanger features a dual electrostatic switch mechanism.

Mammalian cell expression, purification, crystallization and microcrystal data collection of autotaxin/ENPP2, a secreted mammalian glycoprotein.

Structural basis of substrate discrimination and integrin binding by autotaxin.

The structural basis for recognition of base J containing DNA by a novel DNA binding domain in JBP1.

Enabling high-throughput ligation-independent cloning and protein expression for the family of ubiquitin specific proteases.

Idas, a novel phylogenetically conserved geminin-related protein, binds to geminin and is required for cell cycle progression.

Structure-based design of novel boronic acid-based inhibitors of autotaxin.

Investigating a macromolecular complex: the toolkit of methods.

Native mass spectrometry provides direct evidence for DNA mismatch-induced regulation of asymmetric nucleotide binding in mismatch repair protein MutS.

Insights into autotaxin: how to produce and present a lipid mediator.

Mechanism of USP7/HAUSP activation by its C-terminal ubiquitin-like domain and allosteric regulation by GMP-synthetase.

Automatic rebuilding and optimization of crystallographic structures in the Protein Data Bank.

PDB_REDO: constructive validation, more than just looking for errors.

Binding of the J-binding protein to DNA containing glucosylated hmU (base J) or 5-hmC: evidence for a rapid conformational change upon DNA binding.

Cdt1 and geminin in DNA replication initiation.

Glucosylated hydroxymethyluracil, DNA base J, prevents transcriptional readthrough in Leishmania.

Structure-function relationships of autotaxin, a secreted lysophospholipase D.

The polybasic insertion in autotaxin α confers specific binding to heparin and cell surface heparan sulfate proteoglycans.

A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B.

The Geminin and Idas coiled coils preferentially form a heterodimer that inhibits Geminin function in DNA replication licensing.

Timely deposition of macromolecular structures is necessary for peer review.

Autotaxin: structure-function and signaling.

The PDB_REDO server for macromolecular structure model optimization.

The first step of peptide selection in antigen presentation by MHC class I molecules.

CELL DIVISION CYCLE. Competition between MPS1 and microtubules at kinetochores regulates spindle checkpoint signaling.

The structure of the GemC1 coiled coil and its interaction with the Geminin family of coiled-coil proteins.

Steroid binding to Autotaxin links bile salts and lysophosphatidic acid signalling.

Data Mining of Macromolecular Structures.

Structural snapshots of the catalytic cycle of the phosphodiesterase Autotaxin.

Recombinant cloning strategies for protein expression.

Editorial overview: New constructs and expression of proteins.

Glycerophosphodiesterase GDE2 Promotes Neuroblastoma Differentiation through Glypican Release and Is a Marker of Clinical Outcome.

Structural basis of reversine selectivity in inhibiting Mps1 more potently than aurora B kinase.

Structure-Activity Relationships of Small Molecule Autotaxin Inhibitors with a Discrete Binding Mode.

PLA2G16 represents a switch between entry and clearance of Picornaviridae.

Rational Design of Autotaxin Inhibitors by Structural Evolution of Endogenous Modulators.

Understanding inhibitor resistance in Mps1 kinase through novel biophysical assays and structures.

Negative regulation of urokinase receptor activity by a GPI-specific phospholipase C in breast cancer cells.

Vasohibins encode tubulin detyrosinating activity.

Homology-based hydrogen bond information improves crystallographic structures in the PDB.

Glycerophosphodiesterase GDE2/GDPD5 affects pancreas differentiation in zebrafish.

Dynamic kinetochore size regulation promotes microtubule capture and chromosome biorientation in mitosis.

Lysophosphatidic acid produced by autotaxin acts as an allosteric modulator of its catalytic efficiency.

Homology-based loop modeling yields more complete crystallographic protein structures.

Profilin binding couples chloride intracellular channel protein CLIC4 to RhoA-mDia2 signaling and filopodium formation.

Characterization and structure determination of a llama-derived nanobody targeting the J-base binding protein 1.

A crystal structure of the human protein kinase Mps1 reveals an ordered conformation of the activation loop.

Direct binding of Cdt2 to PCNA is important for targeting the CRL4 E3 ligase activity to Cdt1.

Interactions between N-terminal Modules in MPS1 Enable Spindle Checkpoint Silencing.

Building and rebuilding N-glycans in protein structure models.

Crystal structure of the tubulin tyrosine carboxypeptidase complex VASH1-SVBP.

The domain architecture of the protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity.

The Structural Binding Mode of the Four Autotaxin Inhibitor Types that Differentially Affect Catalytic and Non-Catalytic Functions.

Sequence-dependent trafficking and activity of GDE2, a GPI-specific phospholipase promoting neuronal differentiation.

A Global Ramachandran Score Identifies Protein Structures with Unlikely Stereochemistry.

Conserved residues Glu37 and Trp229 play an essential role in protein folding of β-lactamase.

A community proposal to integrate structural bioinformatics activities in ELIXIR (3D-Bioinfo Community).

West-Life: A Virtual Research Environment for structural biology.

Quantitative LC-MS/MS analysis of 5-hydroxymethyl-2'-deoxyuridine to monitor the biological activity of J-binding protein.

LAHMA: structure analysis through local annotation of homology-matched amino acids.

Two β-Lactamase Variants with Reduced Clavulanic Acid Inhibition Display Different Millisecond Dynamics.

The G132S Mutation Enhances the Resistance of β-Lactamase against Sulbactam.

CCD: design constructs for protein expression, the easy way.

New restraints and validation approaches for nucleic acid structures in PDB-REDO.

Target highlights in CASP14: Analysis of models by structure providers.

Imaging Autotaxin with F-Labeled Positron Emission Tomography Ligands.

AI revolutions in biology: The joys and perils of AlphaFold.

Autotaxin impedes anti-tumor immunity by suppressing chemotaxis and tumor infiltration of CD8 T cells.

Structure and function of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family: Tidying up diversity.

Tryptophan depletion results in tryptophan-to-phenylalanine substitutants.

Structure-Based Design of a Novel Class of Autotaxin Inhibitors Based on Endogenous Allosteric Modulators.

Posttranslational modification of microtubules by the MATCAP detyrosinase.

Small Molecule Inhibitor Targeting CDT1/Geminin Protein Complex Promotes DNA Damage and Cell Death in Cancer Cells.

A type IV Autotaxin inhibitor ameliorates acute liver injury and nonalcoholic steatohepatitis.

Author Correction: Tryptophan depletion results in tryptophan-to-phenylalanine substitutants.

Conformational transitions of the Spindly adaptor underlie its interaction with Dynein and Dynactin.

Crystal structure of the phospholipase A and acyltransferase 4 (PLAAT4) catalytic domain.

AlphaFill: enriching AlphaFold models with ligands and cofactors.

Autotaxin facilitates selective LPA receptor signaling.