Shuichi Takayama

Microfluidics for flow cytometric analysis of cells and particles.

Lateral propagation of EGF signaling after local stimulation is dependent on receptor density.

Micro- and nanotechnologies for studying cellular function.

Selective chemical treatment of cellular microdomains using multiple laminar streams.

Passively driven integrated microfluidic system for separation of motile sperm.

Isolation of motile spermatozoa from semen samples using microfluidics.

Rethinking gamete/embryo isolation and culture with microfluidics.

Reversible switching of high-speed air-liquid two-phase flows using electrowetting-assisted flow-pattern change.

Fabrication of microfluidic mixers and artificial vasculatures using a high-brightness diode-pumped Nd:YAG laser direct write method.

Arrays of horizontally-oriented mini-reservoirs generate steady microfluidic flows for continuous perfusion cell culture and gradient generation.

Computerized microfluidic cell culture using elastomeric channels and Braille displays.

Fabrication of reconfigurable protein matrices by cracking.

Computer-controlled microcirculatory support system for endothelial cell culture and shearing.

IVF within microfluidic channels requires lower total numbers and lower concentrations of sperm.

Microfluidic applications for andrology.

Handheld recirculation system and customized media for microfluidic cell culture.

Microscale integrated sperm sorter.

The effect of continuous wavy micropatterns on silicone substrates on the alignment of skeletal muscle myoblasts and myotubes.

A surface-modified sperm sorting device with long-term stability.

Optical imaging in microfluidic bioreactors enables oxygen monitoring for continuous cell culture.

Quantitative measurement and control of oxygen levels in microfluidic poly(dimethylsiloxane) bioreactors during cell culture.

Characterization and resolution of evaporation-mediated osmolality shifts that constrain microfluidic cell culture in poly(dimethylsiloxane) devices.

Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification.

Leakage-free bonding of porous membranes into layered microfluidic array systems.

Tuneable elastomeric nanochannels for nanofluidic manipulation.

Efficient formation of uniform-sized embryoid bodies using a compartmentalized microchannel device.

Assessment of sperm chemokinesis with exposure to jelly coats of sea urchin eggs and resact: a microfluidic experiment and numerical study.

Small volume low mechanical stress cytometry using computer-controlled Braille display microfluidics.

Acoustically detectable cellular-level lung injury induced by fluid mechanical stresses in microfluidic airway systems.

Reversible on-demand cell alignment using reconfigurable microtopography.

DNA linearization through confinement in nanofluidic channels.

Individually programmable cell stretching microwell arrays actuated by a Braille display.

Electromechanical properties of pressure-actuated poly(dimethylsiloxane) microfluidic push-down valves.

Liquid and surfactant delivery into pulmonary airways.

Microfeature guided skeletal muscle tissue engineering for highly organized 3-dimensional free-standing constructs.

A student team in a University of Michigan biomedical engineering design course constructs a microfluidic bioreactor for studies of zebrafish development.

Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids.

Hard top soft bottom microfluidic devices for cell culture and chemical analysis.

Quantitative inference of cellular parameters from microfluidic cell culture systems.

External compression-induced fracture patterning on the surface of poly(dimethylsiloxane) cubes and microspheres.

Microfluidic endothelium for studying the intravascular adhesion of metastatic breast cancer cells.

Microfluidic culture of single human embryonic stem cell colonies.

Unsteady propagation of a liquid plug in a liquid-lined straight tube.

The mechanical properties of a surface-modified layer on poly(dimethylsiloxane).

Patterning alginate hydrogels using light-directed release of caged calcium in a microfluidic device.

Microfluidics cell culture with sensing and SqueezeFluidics.

Bioengineered three-dimensional physiological model of colonic longitudinal smooth muscle in vitro.

Dynamics of liquid plugs of buffer and surfactant solutions in a micro-engineered pulmonary airway model.

Microfluidic hydrodynamic cellular patterning for systematic formation of co-culture spheroids.

High-speed tuning of visible laser wavelength using a nanoimprinted grating optical tunable filter.

Fabrication of two-layered channel system with embedded electrodes to measure resistance across epithelial and endothelial barriers.

Patterned electrode-based amperometric gas sensor for direct nitric oxide detection within microfluidic devices.

Single cell trapping in larger microwells capable of supporting cell spreading and proliferation.

Polymeric aqueous biphasic systems for non-contact cell printing on cells: engineering heterocellular embryonic stem cell niches.

Guided corona generates wettability patterns that selectively direct cell attachment inside closed microchannels.

Simultaneous fabrication of PDMS through-holes for three-dimensional microfluidic applications.

Instantaneous fabrication of arrays of normally closed, adjustable, and reversible nanochannels by tunnel cracking.

Integrated Elastomeric Components for Autonomous Regulation of Sequential and Oscillatory Flow Switching in Microfluidic Devices.

Regulating microenvironmental stimuli for stem cells and cancer cells using microsystems.

The art of reporter proteins in science: past, present and future applications.

Transwells with Microstamped Membranes Produce Micropatterned Two-Dimensional and Three-Dimensional Co-Cultures.

Uniform cell seeding and generation of overlapping gradient profiles in a multiplexed microchamber device with normally-closed valves.

Micropatterning bacterial suspensions using aqueous two phase systems.

Microfluidic platform for chemotaxis in gradients formed by CXCL12 source-sink cells.

High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array.

Multiplexed spectral signature detection for microfluidic color-coded bioparticle flow.

High-density fabrication of normally closed microfluidic valves by patterned deactivation of oxidized polydimethylsiloxane.

Combination of fluid and solid mechanical stresses contribute to cell death and detachment in a microfluidic alveolar model.

Phase-locked signals elucidate circuit architecture of an oscillatory pathway.

Flexible fabrication and applications of polymer nanochannels and nanoslits.

Artificial hematopoietic stem cell niche: bioscaffolds to microfluidics to mathematical simulations.

Epithelium damage and protection during reopening of occluded airways in a physiologic microfluidic pulmonary airway model.

Aqueous biphasic microprinting approach to tissue engineering.

Microprinted feeder cells guide embryonic stem cell fate.

Hi-Fi transmission of periodic signals amid cell-to-cell variability.

Precisely targeted delivery of cells and biomolecules within microchannels using aqueous two-phase systems.

Responses of endothelial cells to extremely slow flows.

Next-generation integrated microfluidic circuits.

Rounded multi-level microchannels with orifices made in one exposure enable aqueous two-phase system droplet microfluidics.

Receptor differential activation and cooperativity better explain cellular preference for different chemoattractant gradient shapes in an EGFR system.

Rethinking in vitro embryo culture: new developments in culture platforms and potential to improve assisted reproductive technologies.

Micro-ring structures stabilize microdroplets to enable long term spheroid culture in 384 hanging drop array plates.

Organs-on-a-Chip: A Focus on Compartmentalized Microdevices.

Secreted CXCL12 (SDF-1) forms dimers under physiological conditions.

384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids.

Constant flow-driven microfluidic oscillator for different duty cycles.

Rapid generation of multiplexed cell cocultures using acoustic droplet ejection followed by aqueous two-phase exclusion patterning.

Real time culture and analysis of embryo metabolism using a microfluidic device with deformation based actuation.

Quantitative analysis of molecular absorption into PDMS microfluidic channels.

Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy.

Lab-on-a-chip biophotonics: its application to assisted reproductive technologies.

Microfluidic automation using elastomeric valves and droplets: reducing reliance on external controllers.

Aqueous two-phase system-derived biofilms for bacterial interaction studies.

Microfluidic systems: a new toolbox for pluripotent stem cells.

Nanoscale squeezing in elastomeric nanochannels for single chromatin linearization.

Super-Resolution Imaging of PDMS Nanochannels by Single-Molecule Micelle-Assisted Blink Microscopy.

Analyzing threshold pressure limitations in microfluidic transistors for self-regulated microfluidic circuits.

Productive chemical interaction between a bacterial microcolony couple is enhanced by periodic relocation.

Localized micro-scale disruption of cells using laser-generated focused ultrasound.

Large-Scale, Ultrapliable, and Free-Standing Nanomembranes.

Microfluidic oscillators with widely tunable periods.

Polymeric Aqueous Biphasic System Rehydration Facilitates High Throughput Cell Exclusion Patterning For Cell Migration Studies.

Room Temperature Phosphorescence of Metal-free Organic Materials in Amorphous Polymer Matrices.