University of Neuchatel
2 ARTICLES PUBLISHED IN JoVE
Environment
Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Tina Wunderlin 1, Thomas Junier 2, Christophe Paul 3, Nicole Jeanneret 3, Pilar Junier 3
1Department of Biological Sciences, Macquarie University, 2Vital-IT group, Swiss Institute of Bioinformatics, 3Laboratory of Microbiology, University of Neuchatel
A method to single out bacterial endospores from complex microbial communities was developed to perform tailored culture or molecular studies of this group of bacteria.
JoVE Journal
Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
Venkatasalam Shanmugabalaji 1, Véronique Douet 1, Birgit Agne 2, Felix Kessler 1
1Laboratory of Plant Physiology, University of Neuchatel, 2Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg
We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.
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