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University of Neuchatel

2 ARTICLES PUBLISHED IN JoVE

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Environment

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Tina Wunderlin 1, Thomas Junier 2, Christophe Paul 3, Nicole Jeanneret 3, Pilar Junier 3
1Department of Biological Sciences, Macquarie University, 2Vital-IT group, Swiss Institute of Bioinformatics, 3Laboratory of Microbiology, University of Neuchatel

A method to single out bacterial endospores from complex microbial communities was developed to perform tailored culture or molecular studies of this group of bacteria.

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JoVE Journal

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
Venkatasalam Shanmugabalaji 1, Véronique Douet 1, Birgit Agne 2, Felix Kessler 1
1Laboratory of Plant Physiology, University of Neuchatel, 2Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg

We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.

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