로그인

University of Neuchatel

2 ARTICLES PUBLISHED IN JoVE

Environment

Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells

Tina Wunderlin¹, Thomas Junier², Christophe Paul³, Nicole Jeanneret³, Pilar Junier³

¹Department of Biological Sciences, Macquarie University, ²Vital-IT group, Swiss Institute of Bioinformatics, ³Laboratory of Microbiology, University of Neuchatel

A method to single out bacterial endospores from complex microbial communities was developed to perform tailored culture or molecular studies of this group of bacteria.

JoVE Journal

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

Venkatasalam Shanmugabalaji¹, Véronique Douet¹, Birgit Agne², Felix Kessler¹

¹Laboratory of Plant Physiology, University of Neuchatel, ²Institut fur Biochemie und Biotechnologie, Martin-Luther-Universitat Halle-Wittenberg

We here present a proven and tested protocol for the purification of chloroplast protein import complexes (TOC-TIC complex) using the TAP-tag. The one-step affinity-isolation protocol can potentially be applied to any protein and be used to identify new interaction partners by mass spectrometry.